Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete
A technology of strawberry root rot and biological control preparations, applied in the field of agricultural intensification, can solve the problems of high production costs, restrictions on the popularization and application of live actinomycete preparations, etc., and achieve the effect of improving quality, improving the quality of agricultural products, and reducing costs
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[0028] 1. Isolation and identification of strains
[0029] The actinomycetes were isolated from the rhizosphere soil of healthy strawberry plants in Houcun, Qilin Town, Nanjing, where continuous cropping obstacles were serious, and the pathogens of strawberry soil-borne root diseases, Fusarium (F. Antagonistic actinomycetes such as V.dahliae, R.solani, and P.fragariae have strong inhibitory effects. After re-screening in the pot test, the highly effective antagonistic actinomycete B04 strain was finally obtained, and the biological identification of the strain was carried out, and the strain was stored in a 4 refrigerator.
[0030] The biocontrol strain B04 belongs to S. hygroscopicus, and its main biological characteristics are: the best growth temperature on PDA medium is 28°C, and it can be cultured for 5-7 days to form colonies with a diameter of 2-4mm, and the colonies are in the early stage White, gray in the later stage, with water absorption phenomenon, spore filament...
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