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Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete

A technology of strawberry root rot and biological control preparations, applied in the field of agricultural intensification, can solve the problems of high production costs, restrictions on the popularization and application of live actinomycete preparations, etc., and achieve the effect of improving quality, improving the quality of agricultural products, and reducing costs

Inactive Publication Date: 2015-03-11
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] At present, the conventional production of live actinomycete preparations mostly uses high-priced agricultural and sideline products such as soybean flour, corn flour, and yeast powder as raw materials, and the production cost is relatively high, which limits the further popularization and application of live actinomycete preparations. According to reports Raw materials for the production of live actinomycete preparations account for 30-40% of the entire production cost. Therefore, the selection of low-cost raw materials is the key to the commercial production and promotion of live actinomycete preparations

Method used

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  • Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete
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  • Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete

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Embodiment Construction

[0028] 1. Isolation and identification of strains

[0029] The actinomycetes were isolated from the rhizosphere soil of healthy strawberry plants in Houcun, Qilin Town, Nanjing, where continuous cropping obstacles were serious, and the pathogens of strawberry soil-borne root diseases, Fusarium (F. Antagonistic actinomycetes such as V.dahliae, R.solani, and P.fragariae have strong inhibitory effects. After re-screening in the pot test, the highly effective antagonistic actinomycete B04 strain was finally obtained, and the biological identification of the strain was carried out, and the strain was stored in a 4 refrigerator.

[0030] The biocontrol strain B04 belongs to S. hygroscopicus, and its main biological characteristics are: the best growth temperature on PDA medium is 28°C, and it can be cultured for 5-7 days to form colonies with a diameter of 2-4mm, and the colonies are in the early stage White, gray in the later stage, with water absorption phenomenon, spore filament...

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Abstract

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

Description

technical field [0001] The invention relates to an antagonistic actinomycete for preventing and controlling continuous-cropping strawberry root rot and a method for producing bio-control live bacteria preparations by solid fermentation of vermicompost and wheat bran, belonging to the technical field of agricultural intensification. Controlling soil-borne diseases of strawberry in continuous cropping. Background technique [0002] Strawberry (Fragaria ananassa) is a perennial herbaceous plant. The fruit is soft and juicy, rich in nutrition and multivitamins. It is known as the "Queen of Fruits". It is popular because of its strong adaptability, short growth period, early results, and high economic benefits. Strawberry is cultivated in facility continuous cropping. It only takes 2 to 3 years for strawberry continuous cropping to develop from sporadic occurrence to large-scale disease, and the incidence rate of continuous cropping and heavy cropping is as high as 80%, which se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/55
CPCA01N63/00C12N1/205C12R2001/55
Inventor 王世梅沈婷王辰徐阳春沈标韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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