Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease

A technology for root-knot nematode disease and rice sheath blight, which is applied in the directions of nematicides, bacteria, biocides, etc. to increase income and save expenses

Active Publication Date: 2010-03-17
NANJING AGRICULTURAL UNIVERSITY
View PDF1 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Technical problem: In view of the current situation that there is no biocontrol agent with high control effect on vegetable root-knot nematode disease and rice sheath blight in the prior art, provide and develop a single biological control agent for controlling vegetable root-knot nematode disease and rice sheath blight. Antibacterial strains and their agents, which have a control effect of more than 50% on these two important soil-borne diseases, and have the function of increasing production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease
  • Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease
  • Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Preparation of AT31 biocontrol agent

[0019] The invention relates to a biocontrol bacterial strain AT31 for preventing and treating root-knot nematode, bacterial wilt, blight and rice sheath blight of greenhouse vegetables, which belongs to the genus Bacillus cereus and was deposited in China Microbiology on June 18, 2009. General Microbiology Center of the Culture Collection Management Committee, the culture preservation number is CGMCC NO.3128. It was isolated from the soil of Zijinshan forest in Nanjing.

[0020] The above-mentioned biocontrol strain AT31 was shaken and cultivated in PDA culture medium at 30°C and 180rpm for 20-24h, then centrifuged at 6000rpm for 10min, and the wet bacteria and bacteria preservation solution of AT31 (patent No. ZL02112559.7, published patent No. 1:40 to prepare bacterial agent, the total concentration of viable bacteria in the finished bacterial agent is 1×10 9 ~1×10 10 cfu / ml, save for future use. The bacterial cell preser...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease, belonging to the technical field of plant protection. The bacterial strain AT31 is shaking cultured in PDA culture solution at 37 DEG C and 180rpm for 20-24 hours, then is subject to centrifugation at 6000rpm for 10min, a finished bactericide product thereof has a total viable bacteria concentration of 1*10<9>-1*10<10>cfu / ml, can be used by pouring at roots when transplanting vegetable seedlings after being diluted by 100 times, or be manufactured into microbial fertilizer to be used a week before the vegetable seedlings are transplanted, and has a prevention effect of 58-82% for root knot nematode disease of tomato, loofah, cucumber and other vegetables. The bactericide is diluted by 100 times to be used in spray treatment on rice stem base, has a prevention effect of 64.7% for rice sheath blight disease, and can increase the yield by 22.33%. And the bactericide can also cure vegetable bacterial wilt and epidemic diseases, and can improve soil hardening phenomenon.

Description

1. Technical field [0001] The invention relates to a biocontrol bacterial strain AT31 for preventing and treating vegetable root-knot nematode and rice sheath blight, which belongs to the technical field of plant protection and is specially used for pollution-free prevention and control of vegetable root-knot nematode and rice sheath blight. 2. Background technology [0002] Vegetable root-knot nematode disease caused by root-knot nematode (Meloidogyne spp.) infection is one of the most serious plant diseases at home and abroad, which can damage cucumber, wax gourd, watermelon, loofah, tomato, pepper, eggplant, potato, lettuce, spinach , carrots, cabbage, leguminous vegetables and most other vegetables. Plant diseases caused by plant parasitic nematodes attacking and parasitizing, the damaged plants can affect the normal growth and development due to the invading nematodes absorbing nutrients in the body; the secretions of the nematodes in the metabolic process can also stim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00A01P3/00C12R1/085
Inventor 郭坚华刘红霞魏利辉陈云
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap