Streptomyces having inhibitory effect on phytophthorasojae root rot fungi, biocontrol preparation and preparation method of biocontrol preparation

A technology of soybean Phytophthora and biocontrol preparations, applied in the field of agricultural biology, can solve the problems of few oomycete diseases and few reports, and achieve the effects of protecting soil and environmental safety, improving soybean quality, and saving costs

Active Publication Date: 2017-02-15
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, there are not many reports on the use of streptomyces agents to prevent and control oomycete diseases, especially the effective Streptomyces agents against Phytophthora sojae are rarely reported

Method used

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  • Streptomyces having inhibitory effect on phytophthorasojae root rot fungi, biocontrol preparation and preparation method of biocontrol preparation
  • Streptomyces having inhibitory effect on phytophthorasojae root rot fungi, biocontrol preparation and preparation method of biocontrol preparation
  • Streptomyces having inhibitory effect on phytophthorasojae root rot fungi, biocontrol preparation and preparation method of biocontrol preparation

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Embodiment Construction

[0025] Below in conjunction with embodiment and experiment the present invention will be further described:

[0026] Streptomyces dorenlacusensis (Streptomyces dorenlacusensis) M1-4 is Streptomyces dorenlacusensis M1-4 which has inhibitory effect on soybean Phytophthora root rot pathogen of the present invention. No. 3, No. 1 Yard, Beichen West Road, District, the preservation number is CGMCC NO.11789.

[0027] The screening method of Streptomyces dorenlacusensis M1-4 comprises the steps of:

[0028] (1) Collect sediment samples from lakes, dry them in a dark and ventilated place, and grind them into powder with a mortar;

[0029] (2) Utilize dilution method to separate, soil sample is put into the bottle that glass beads and sterile water are housed, shake, after standing still, dilute, preparation mass concentration is 10 -3 、10 -4 、10 -5 Dilution solution, draw the dilution solution and add it dropwise to the Gaoshi No. 1 medium plate with cycloheximide (50 μg / ml) and n...

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Abstract

The invention discloses streptomyces having an inhibitory effect on phytophthorasojae root rot fungi, a biocontrol preparation and a preparation method of the biocontrol preparation, and belongs to the field of agricultural biotechnology. The strain (streptomyces) is streptomyces dorenlacusensis M1-4. The biocontrol preparation is obtained by inoculating a fermentation medium, which mainly takes soybean cake meal and wheat bran as main raw materials, with the streptomyces dorenlacusensis M1-4 which is subject to enlarged cultivation and by conducting fermentation culture, wherein the total number of spores of the biocontrol preparation is greater than or equal to 10<10> spores/g. From the key problem of preventing and controlling soybean diseases by virtue of bio-pesticide antagonistic microbe, the streptomyces dorenlacusensis M1-4 biocontrol preparation, which is capable of significantly relieving the occurrence of phytophthorasojae root rot, is researched and prepared, and the field preventing and controlling rate of the biocontrol preparation reaches 60% or above. With the application of the biocontrol preparation, the growth of soybean rhizosphere pathogenic microorganisms can be inhibited. Meanwhile, the biocontrol preparation can greatly reduce cost and can achieve a relatively good preventing effect.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a streptomycete, a biocontrol agent and a preparation method thereof. Background technique [0002] Soybean (Glycine max) is an important economic crop, rich in plant protein, used to make various soybean products, extract soybean oil, brew soy sauce and extract protein. Okara, or soybeans ground into a meal, are also commonly used in livestock feed. Soybean production is affected by many diseases. Soybean blight, also known as soybean Phytophthora root rot, is an oomycete disease caused by Phytophthorasojae infection. It is a multi-cycle soil-borne disease that spreads quickly, damages a large area, and is difficult to control. It is a dangerous disease in soybean production and is recognized as the second most serious soybean disease after soybean cyst nematode disease. The rate of dead plants at the seedling stage caused by Phytophthora sojae root rot r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 高亚梅韩毅强王彦杰王伟东张蕾
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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