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Compound microorganism bacterium agent for preventing and treating fruit and vegetable downy mildews and preparation method thereof

A technology of compound microbial inoculants and microbial strains is applied in the fields of botanical equipment and methods, biocides, and chemicals for biological control, etc. Excessive chemical pesticide residues and other problems have achieved the effects of strong film-forming ability, reduction of grape downy mildew, and favorable export revenue.

Active Publication Date: 2015-06-03
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a compound microbial bacterial agent for preventing and treating fruit and vegetable downy mildew and a preparation method thereof aiming at solving the problems of difficulty in preventing and controlling grape and cucumber downy mildew in agricultural production, high cost, excessive chemical pesticide residues and the like by using biological control at present. It can prevent grape downy mildew and cucumber downy mildew. The compound bacterial agent is a suspending agent compounded by two biocontrol strains with different action modes. The control effect on grape and cucumber downy mildew is 88.76 %above

Method used

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  • Compound microorganism bacterium agent for preventing and treating fruit and vegetable downy mildews and preparation method thereof
  • Compound microorganism bacterium agent for preventing and treating fruit and vegetable downy mildews and preparation method thereof
  • Compound microorganism bacterium agent for preventing and treating fruit and vegetable downy mildews and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Strain isolation and identification

[0032] 1. The Bacillus cereus BCJB01 and BMJBN02 involved in the present invention are isolated from the cabbage field in Gaotang County, Liaocheng City, Shandong Province. The strain preservation number is CGMCC NO.10477, which has a unique function of decomposing zoospores of downy mildew

[0033] Bacillus megaterium BMJBN02 is isolated from the soil of leek field in Shouguang, Shandong Province. The strain preservation number is CGMCC NO.10478. It has the function of deforming the sporangia of downy mildew.

[0034] 2. Strain identification

[0035] The strains BCJB01 and BMJBN02 were identified as Bacillus cereus and Bacillus megaterium respectively by the combination of physiological and biochemical characteristics determination and 16S rDNA sequence analysis. At the same time, the isolated and purified single colonies were identified by V.P test, bacterial film formation test, lecithinase test, starch hydrolysis test, casein ...

Embodiment 2

[0037] Preparation of compound microbial suspension

[0038] 1. Preparation of seed liquid: Inoculate the above-mentioned biocontrol bacteria Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 into liquid LB medium respectively, shake and culture at 30°C and 220rpm for 12-18 hours, and take samples on the ultra-clean workbench Measure the OD value at 600nm, and adjust to zero with uninoculated liquid LB medium during the determination process;

[0039] 2 Preparation of fermentation broth:

[0040] ① Preparation of Bacillus cereus BCJB01 fermentation broth: When the OD value of the culture broth is 0.5-0.8, add the seed solution into the culture tank with the fermentation medium at a volume ratio of 1:100. The culture temperature is 30°C, the rotation speed is 200r / min, the fermentation time is 24~36h, the pH of the fermentation broth is maintained at 7.0, the dissolved oxygen is 20%, and the ventilation volume is 5~7m 3 / hour, tank pressure 0.05~0.1KPa, sporulation rate ...

Embodiment 3

[0057] Control of Downy Mildew with Composite Microbial Suspension Concentrate

[0058] 1 Field plot test of compound microbial suspension concentrate Ⅰ on grape downy mildew

[0059] The experiment was carried out in the vineyard of the Biological Center of Shandong Academy of Sciences, and the variety was Zeshan No. 1.

[0060] The test was designed according to the field test guidelines of the Institute of Drug Control of the Ministry of Agriculture, and there were 4 treatments in total: the compound microbial suspension concentrate I was diluted with water to 100 and 600 times liquid, 20% propamocarb aqueous solution was 600 times liquid, and water was used as the control. The spray method was used to apply the pesticide after 16:00 in the afternoon, and 10 plants were treated for each treatment, with 3 repetitions and a randomized block design. 10 new vines were randomly surveyed in each plot, 10 leaves were surveyed from top to bottom, and the number of diseased leaves ...

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Abstract

The invention discloses a compound microorganism bacterium agent for preventing and treating the fruit and vegetable downy mildews. The compound microorganism bacterium agent contains two effective bacterium components: Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. The grape downy mildew and the cucumber downy mildew can be prevented and treated. The compound microorganism bacterium agent is a suspending agent formed by compounding two biocontrol strains in different action modes, and the control efficiency for the grape and the cucumber downy mildews is greater than 88.76%.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a composite microbial agent for preventing and treating fruit and vegetable downy mildew and a preparation method thereof. Background technique [0002] Downy mildew is serious damage to Cucurbitaceae melon crops. The causative bacterium of melon downy mildew is Pseudoperonosporacubensis (Berk.et Curt.) Rostov]. The most frequently infected plants are the genus Cucumber and Melon. The cultivated cucumbers in more than 80 countries and regions are related to 50 Melon is widespread in many countries and regions. Under natural conditions, the pathogen not only infects melon and cucumber, but also causes harm to 12 kinds of melon crops such as watermelon, pumpkin, loofah, wax gourd, gourd and snake gourd of Cucurbitaceae (Yang Liuyan, Xu Yongyang, etc. Melon downy mildew Research progress [J], Chinese Melon and Vegetables, 2011, 24(3): 38-43). [0003] Grape downy mildew is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/30A01P3/00
Inventor 李纪顺王贻莲扈进冬杨合同谢雪迎
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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