Bacterial strain B211 for preventing cotton verticillium wilt

A cotton verticillium wilt and bacterial strain technology, applied in the direction of fungicides, fungi, and methods based on microorganisms, can solve problems such as hazards, achieve the effects of increasing income, saving expenses, and improving the control effect

Active Publication Date: 2008-10-08
南京农大生物源农药创制有限公司
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disease resistance of cotton varieties in my country can only reach the level of disease resistance at present, resulting in the continuous prevalence of the disease in the case of suitable environmental conditions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain B211 for preventing cotton verticillium wilt
  • Bacterial strain B211 for preventing cotton verticillium wilt
  • Bacterial strain B211 for preventing cotton verticillium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] 1 Identification of strains

[0021] The biocontrol strain B221 used to control cotton Verticillium dahliae Kleb. was produced from a cotton field in Dingzi'an Village, Juegang Town, Rudong County, Nantong City. The roots of the strain are isolated, and the inventor has studied the flat plate antagonistic activity, cellulase, protease, chitinase activity and utilization of phosphorus and nitrogen elements of the bacterial strain B221, and found that the bacterial strain B221 is effective against cotton Verticillium dahliae ( Verticilliumdahliae) has strong plate antagonistic activity, but does not have protease, cellulase and chitinase activity, and cannot utilize phosphorus and nitrogen elements. The bacterial strain is cultivated in PDA medium and stored at 4 ° C on the PDA slant. The inventor also carried out greenhouse control effect and growth-promoting experimental research on it, and found that the B221 strain preparation has a good effect of disease prevention a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to biocontrol bacterial strain B221 which can prevent the verticillum wilt of cottons, and the biocontrol bacterial strain B221 belongs to the technical field of the agricultural crop. The biocontrol bacterial strain B221 is identified as the chaetomium globosum. The preservation number of the strain is CGMCC NO.2493. The strain is shaken and cultured in PDA culture solution with 25 DEG C 200rpm for 7 days and is filtered through eight-layer of sterilization gauze to obtain the spore suspending liquid, the total concentration of the viable bacteria in the finished product is 10 <9> spores/ milliliters, therefore to obtain the biocontrol bacterial agent. The bacterial strain is preserved in the PDA inclined surface at 4 DEG C, the bacterial strain B221 represents better flat plate antagonism effect, greenhouse prevention effect and growth facilitation effect. The experiment shows that the greenhouse prevention effect of the bacterial strain B221 achieves 58.96 percent, the biomass increases by 51.20 percent; the biocontrol bacterial strain B221 has greater potentiality to be developed into the commercialized viable bacteria biocontrol agent.

Description

1. Technical field [0001] The invention relates to a single bacterial strain for preventing and treating cotton verticillium wilt, belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and treatment of cotton verticillium wilt. 2. Background technology [0002] Verticillium dahliae Kleb., the cotton verticillium dahliae caused by the fungus Verticillium dahliae Kleb., is a worldwide and devastating disease, known as the "cancer" of cotton. Since it was first reported in the United States in 1914, it has now spread to all major cotton regions in the world. Our country has been imported from the United States since 1935 when the Sri Lankan cotton was introduced, and it has continued to expand with the cotton seed allocation and transportation. In recent years, cotton verticillium wilt has been continuously prevalent in various cotton areas in China, especially in 2002-2003, it was prevalent in a large area...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 郭坚华徐莉莉鲁莎
Owner 南京农大生物源农药创制有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap