Bacterial strain B211 for preventing cotton verticillium wilt

Abstract

The invention relates to biocontrol bacterial strain B221 which can prevent the verticillum wilt of cottons, and the biocontrol bacterial strain B221 belongs to the technical field of the agricultural crop. The biocontrol bacterial strain B221 is identified as the chaetomium globosum. The preservation number of the strain is CGMCC NO.2493. The strain is shaken and cultured in PDA culture solution with 25 DEG C 200rpm for 7 days and is filtered through eight-layer of sterilization gauze to obtain the spore suspending liquid, the total concentration of the viable bacteria in the finished product is 10 <9> spores / milliliters, therefore to obtain the biocontrol bacterial agent. The bacterial strain is preserved in the PDA inclined surface at 4 DEG C, the bacterial strain B221 represents better flat plate antagonism effect, greenhouse prevention effect and growth facilitation effect. The experiment shows that the greenhouse prevention effect of the bacterial strain B221 achieves 58.96 percent, the biomass increases by 51.20 percent; the biocontrol bacterial strain B221 has greater potentiality to be developed into the commercialized viable bacteria biocontrol agent.

Description

1. Technical field [0001] The invention relates to a single bacterial strain for preventing and treating cotton verticillium wilt, belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and treatment of cotton verticillium wilt. 2. Background technology [0002] Verticillium dahliae Kleb., the cotton verticillium dahliae caused by the fungus Verticillium dahliae Kleb., is a worldwide and devastating disease, known as the "cancer" of cotton. Since it was first reported in the United States in 1914, it has now spread to all major cotton regions in the world. Our country has been imported from the United States since 1935 when the Sri Lankan cotton was introduced, and it has continued to expand with the cotton seed allocation and transportation. In recent years, cotton verticillium wilt has been continuously prevalent in various cotton areas in China, especially in 2002-2003, it was prevalent in a large area...

AI Technical Summary

Problems solved by technology

However, the disease resistance of cotton varieties in my country can only reach the level of disease resistance at present, resulting in the continuous prevalence of the disease in the case of suitable environmental conditions.

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