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Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof

A technology of pepper bacterial wilt and organic fertilizer, applied in the field of intensive agricultural production, can solve the problem of low control effect, achieve the effect of saving expenses and increasing income

Active Publication Date: 2010-08-18
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, there are mainly different methods for the prevention and treatment of pepper bacterial wilt, such as crop rotation, chemical control and selection of disease-resistant varieties, but the control effect is low.

Method used

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  • Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof
  • Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof

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Experimental program
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Embodiment Construction

[0019] (1) Isolation and identification of strains

[0020] The pathogenic bacteria Ralstonia solanacearum (Ralstonia solanacearum) of pepper bacterial wilt (Germany Culture Collection Center (DSMZ), Ralstonia solanacearum (DSM No.9544), the screening of antagonistic strains of pepper bacterial wilt and the control effect in the field Determination, "Chinese Biological Control" 2001, the 17th volume, the 03rd phase, Guo Jianhua etc.) is indicator bacteria from isolated antagonistic bacteria from the rhizosphere soil of healthy pepper plants in the capsicum bacterial wilt diseased field. Finally, the highly effective antagonistic strain a45 was screened out through pot experiments, the genus was identified, and the strain was stored at -70°C in 30% glycerol. Antagonistic bacteria a45, belonging to Bacillus subtilis, the main biological characteristics are: Gram stain positive G + , the shape of the cells is rod-shaped, the spores are mesozoic or submesozoic, oval, the cysts ar...

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Abstract

The invention relates to an antagonistic bacterium for controlling the bacterial wilt of capsicum and a microorganism organic fertilizer thereof, belonging to an agricultural intensified production technology. An antagonistic bacterium (bacillus subtilis a45) which plays an obvious antagonism role in pathogenic bacteria of the bacterial wilt of the capsicum is obtained by separation, and the disease-resistant microorganism organic fertilizer is prepared from the antagonistic bacterium and organic compost; and the fertilizer contains more than 2.0*109 a45 per gram, the total nitrogen content is 33.1g kg<-1>, the total phosphorus content is 13.3g kg<-1> and the total potassium content is 21.3g kg<-1>. Tests prove that the antagonistic bacterium can be rapidly bred after the microorganism organic fertilizer is applied to soil and forms a preponderant population in soil; and the preventing and treating rate on the bacterial wilt of the capsicum is as high as 70 percent.

Description

1. Technical field [0001] The invention relates to an antagonistic bacteria capable of preventing and treating pepper bacterial wilt and a microbial organic fertilizer made by using the antagonistic bacteria, which belongs to the technical field of agricultural intensive production and is specially used for overcoming and eliminating the problem of pepper bacterial wilt. 2. Background technology [0002] Pepper bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum. Once it occurs, it usually causes 20%-30% of peppers to die, and in severe cases, it can reach more than 50%, or even crop failure. At present, there are mainly different methods for the prevention and treatment of pepper bacterial wilt, such as crop rotation, chemical control and selection of disease-resistant varieties, but the control effect is low. With the development of green agriculture, the focus of the prevention and control of pepper bacterial wilt has gradually shifted to the comprehen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C05G3/00C12R1/125
CPCY02P60/21
Inventor 沈其荣江欢欢沈标杨兴明冉炜
Owner NANJING AGRICULTURAL UNIVERSITY
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