Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof
A technology of pepper bacterial wilt and organic fertilizer, applied in the field of intensive agricultural production, can solve the problem of low control effect, achieve the effect of saving expenses and increasing income
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[0019] (1) Isolation and identification of strains
[0020] Screening of pepper bacterial wilt pathogen Ralstonia solanacearum (DSMZ), Ralstoni a solanacearum (DSM No.9544), the antagonistic strain of pepper bacterial wilt and its field control effect Determination, "China Biological Control" 2001, Vol. 17, No. 03, Guo Jianhua, etc.) is the indicator bacteria isolated from the rhizosphere soil of healthy pepper plants from the field of pepper bacterial wilt disease. Finally, a pot experiment was conducted to screen out the highly effective antagonistic strain a45, and the species was identified. The strains were stored in 30% glycerol at -70°C. Antagonistic antibacterial a45, belonging to Bacillus subtilis (Bacillus subtilis), main biological characteristics: Gram staining positive G + , The cell shape is rod-shaped, spores are mesophytic or nearly mesophytic, oval, cysts are not enlarged, and no accompanying spore crystals; fermented glucose, fructose, arabinose, mannose, mannit...
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