Bacillus subtilis, and breeding method and application thereof

The technology of Bacillus aureus and thuringiensis spore is applied in the field of biopesticide preparation, which can solve the problems of environmental pollution, affecting the quality of life, excessive pesticide residues, etc., and achieve the effects of high genetic stability, good inhibitory effect and broad application prospects.
CN104845897AInactive Publication Date: 2015-08-19CHINA THREE GORGES UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
CHINA THREE GORGES UNIV
Publication Date
2015-08-19
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention provides Bacillus subtilis ML1 which is preserved in China Center for Type Culture Collection on May 5, 2014, with an accession number of CCTCC No: M2014181. A breeding method for the Bacillus subtilis ML1 comprises the following steps: adding sterile water into the tissue fluid of leaves of Myricaria laxiflora and carrying out dilution to obtain 7 concentration gradients; coating medium plates with the diluted tissue fluid, invertedly placing the medium plates in a thermotank and carrying out culture at 30 DEG C; after bacterium colonies have grown on the plates, carrying out shake cultivation at a constant temperature of 30 DEG C so as to obtain bacterium culture solutions, sterilizing the bacterium culture solutions, mixing the bacterium culture solutions with glycerin and preserving the obtained mixture in a refrigerator with a temperature of -86 DEG C; subjecting the preserved Bacillus subtilis strain to streak inoculation in a slant medium and carrying out culture at 30 DEG C for 12 h; and subjecting the strain to streak inoculation in a medium and carrying out culture at 30 DEG C for 18 h so as to obtain the Bacillus subtilis ML1. The Bacillus subtilis ML1 is applied to prevention and treatment of soft rot disease of konjak and provides a novel option for development of a novel biological pesticide variety and novel biological control inocula.
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Description

technical field

[0001] The invention relates to a bacillus thuringiensis and its breeding method, and its application in the field of preparing biological pesticides. Background technique

[0002] Soft rot, commonly known as "half-sided madness", is the most serious disease in konjac production. Konjac soft rot belongs to the genus Erwinia (Erwinia), a Gram-negative bacterium, Erwinia carotovora subsp.carotovora, which can develop diseases during the cultivation and storage periods , affecting yield and quality. The konjac planting industry loses a lot due to soft rot every year.

[0003] For centuries, chemical pesticides have played a huge role in the control of plant diseases and insect pests, and have made important contributions to increasing, stabilizing and improving agricultural production. However, due to excessive application and abuse of pesticides, soil fertility decline and environmental pollution. Especially in recent years, due to excessive pesticide resid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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