Rapid propagation method for plateaus of alliums

A technology for allium plants and bulb discs, which is applied in the agricultural field, can solve the problems that it is difficult to meet large-scale production, and the tissue culture fast propagation rate of allium plants is not high, and achieves low price cost, low time cost, and high genetic stability. Effect

Active Publication Date: 2016-01-27
吉林省蔬菜花卉科学研究院
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the rapid multiplication rate of Allium plant tissue culture i

Method used

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  • Rapid propagation method for plateaus of alliums
  • Rapid propagation method for plateaus of alliums
  • Rapid propagation method for plateaus of alliums

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 Tiller onion stem tip multiplication

[0033] The shoot tip of tillering onion was inoculated in different concentrations of 6-BA, NAA combination and 6-BA, IAA combination medium (Table 1). And NAA in the induction of proliferation, there is a big difference. Among them, the proliferating seedlings induced by the combination of 6-BA and NAA hormones grow better, and have a higher differentiation coefficient. After 4 weeks of culture, the average proliferation coefficient can reach 3.87; although the combination of 6-BA and IAA hormones can also achieve shoot tip Proliferation, but the proliferated seedlings obtained are thin and weak, with low differentiation coefficient, and albino seedlings appear (such as figure 1 ). It can be seen that the effect of IAA is not as good as that of NAA. Therefore, MS+6-BA0.3mg / L+NAA0.1mg / L medium was determined to be the best medium for shoot tip proliferation.

[0034]

[0035] Note: MS is the basic medium.

Embodiment 2

[0036] Embodiment 2 Tiller onion bulb disc multiplication

[0037] Take one tillering onion, remove the corky part of the tillering onion bulb disk, keep the bulb disk with a diameter of 0.4-1 cm centered on the central growth point and the bulb wrapped on the upper part, and obtain the center part of the tillering onion. Rinse the center of the tillering onion with running water for 0.5h, soak and disinfect with 75% alcohol for 1min, then soak and disinfect with 1% NaClO solution for 15-20min, rinse with sterile water 3-5 times to remove residual NaClO, and blot dry with filter paper water backup (eg figure 2 ).

[0038]Then remove the upper bulb, keep the bulb disk including the main growth point, cut the bulb disk into 4 parts, remove the main growth point and the main growth point bulb disk, and inoculate them on MS as the basal medium respectively, add 6-BA ( 0.2-0.8mg / L), NAA (0.1-2.0mg / L), PH adjusted to 5.8 value-added culture medium to induce clustered buds. Under...

Embodiment 3

[0043] Example 3 Tiller onion virus-free seedling test tube balling

[0044] Take the tip of the tiller onion, wash it with running water for half an hour, soak it in 75% alcohol for 1 minute, then soak it in 1% NaClO solution for 15-20 minutes, rinse it with sterile water for 3-5 times to remove the residual NaClO, and absorb it with filter paper. Dry the water for backup, peel off the part of the growth point of the tiller onion shoot tip <0.3mm under the microscope, and induce seedlings in the starting medium with MS as the base medium and supplemented with 6-BA and IAA.

[0045] After the stem tips became seedlings, sucrose was used as the basic carbon source to set 5 carbon source sugar concentrations of 30mg / L, 45mg / L, 60mg / L, 75mg / L, and 90mg / L, to explore the effects of different sugar concentrations on the tillering onion test-tube plantlets. For the effect of bulbing, the sugar concentration in the best bulbing medium was screened, and it was found through cultivatio...

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Abstract

The invention discloses a rapid propagation method for plateaus of alliums. The method comprises the following steps: cleaning and disinfecting central parts of the alliums; transversely cutting the central parts 3-5 millimeters away from bottom ends to remove upper scales; longitudinally partitioning into four parts along a vertical tangent line of a main growth point; removing the main growth point and the plateau where the main growth point is positioned; inducing multiple shoots; culturing into seedlings. Through optimization of the culturing process, a rapid propagation system for the plateaus of the alliums is established and a large quantity of virus-free tube seedlings are obtained. The system has the advantages of short proliferation time, large proliferation coefficient and high genetic stability. Through the system, rapid propagation of the alliums can be realized. Industrialized production of allium virus-free tube seedlings can be ensured with a low price cost and a low time cost, and development of the allium industry is promoted.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for rapid propagation of Allium bulb discs. Background technique [0002] Plants of the genus Allium use tiller bulblets for reproduction in production, which belongs to asexual reproduction. Long-term asexual reproduction leads to severe viral diseases caused by a single virus infection or multiple virus co-infection. After the plant is infected, the growth potential is weak, the plant is short, the tillers are reduced, and the plant becomes smaller due to degeneration, resulting in a significant reduction in yield and quality, which has brought serious obstacles to production, consumption and export earnings. Most of the detoxification techniques of Allium plants use stem tip culture, which has achieved good results, but the reproduction rate is not high, so it is difficult to be used in large-scale production. [0003] Tiller onion belongs to the All...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王秀峰张悦马艺荞王学国王健鹂于永辉聂楚楚冯博
Owner 吉林省蔬菜花卉科学研究院
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