Combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos

A technology of volatile compounds and anthracnose, which is applied in the field of combined volatile compounds and its application in the prevention and treatment of mango postharvest anthracnose, can solve the problems of pesticide residues, human health hazards, easy to produce drug resistance, etc., and achieve good biological control value, good antifungal activity

Pending Publication Date: 2018-07-06
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anthracnose (Colletotrichum Corda) fungi are a class of globally distributed plant pathogens, and the mango anthracnose caused by it is one of the most important diseases on mangoes. It forms a latent infection on immature mangoes. The disease in the storage and transportation links caused huge economic losses
At present, mango anthracnose mainly relies on chemical agents for prevention and control, but the large-scale use of chemical agents can easily cause pesticide residues and cause harm to human health. At the same time, long-term use of a single chemical pesticide is likely to produce drug resistance

Method used

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  • Combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos
  • Combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos
  • Combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is used to illustrate the inhibitory effect of different combinations of volatile substances on the mycelium of anthracnose.

[0023] The method for determining the inhibitory effect of different combinations of volatile substances on the mycelium of anthracnose: Place 4 small petri dishes (diameter 6cm, height 1.5) on the bottom of a large petri dish with a diameter of 15cm and a height of 3cm (total volume is about 500mL) cm), and place a small container with a capacity of 1 mL in the center of the large petri dish, and place a 15mm×15mm square filter paper inside the small container. 4 small petri dishes contain about 5 mL of PDA medium, and inoculate the anthracnose mycelium block with a diameter of 6mm in the center of the petri dish, and then add the combined volatile substances corresponding to the concentration and volume content to the small container in the center of the large petri dish. The combined concentration setting and the amount to be added ...

Embodiment 2

[0032] This example is used to illustrate the inhibitory effects of different combinations of volatile substances on the germination of anthracnose conidia.

[0033] The method of determining the inhibitory effect of different combinations of volatile substances on the germination of anthracis spores: Place 4 small petri dishes (diameter 6cm, diameter 6cm, diameter 6cm, 1.5cm high), and place a small container with a capacity of 1 mL in the center of the large petri dish, and place a 15mm×15mm square filter paper inside the small container. 4 small petri dishes hold about 5mL PDA medium, and add 20μL 5×10 to PDA medium 5 Spore suspension per mL, spread it evenly and blow dry the water. Then add the combined volatile substances corresponding to the concentration and volume content to the small container in the center of the large petri dish, and immediately seal the large petri dish with a parafilm to prevent leakage of the volatile substances. Set 3 replicates for each concentra...

Embodiment 3

[0038] This example is used to illustrate the inhibitory effects of different combinations of volatile substances on mango anthracnose lesions.

[0039] Determination of the inhibitory effects of different combinations of volatile substances on anthracnose lesions on mangoes: Put 10 Tainong No. 1 small mangoes in a glassware (4L), stab and inoculate anthracnose conidia suspension, concentration 1×10 6 Pcs / mL, each mango inoculates 1 point, each point is inoculated with 10μL of spore suspension, 24h after inoculation, the concentration of each combination is 1μL·L -1 , 10μL·L -1 And 100μL·L -1 Fumigation treatment under the condition of concentration, 3 repetitions for each concentration, namely 3 glassware, after 48 hours of fumigation, take the mango out and put it into a cardboard box padded with wet paper. Observe the development of anthracnose lesions every day. It is about 8th from the inoculation. Observe the results every day, count the incidence rate, measure the diameter ...

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Abstract

The invention provides a combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos. According to the invention, the application of the combined volatile compound in preventing and treating the anthracnose of the postharvest mangos is found for the first time. The combined volatile compound can act on colletotrichum mycelium and conidium by volatilization in a gas mode without contacting the fruits in a sealed environment to inhibit mycelia growth, conidia germination and disease speckle expansion; thus, an effect of preventing and treatingthe mango anthracnose is achieved. As the combined volatile compound is extremely easy to volatilize, the compound left on the surfaces of the mangos after steaming treatment is easy to volatilize; thus, an effect on mango quality and harm to human body health are reduced. The invention determines matching and use concentrations of 7 groups of different combined volatile compounds with an effect of preventing and treating the mango anthracnose. Compared with existing preservation chemicals, the combined volatile compound has the advantages of good preventing effect, low residue and very good development and application prospects.

Description

Technical field [0001] The invention belongs to the field of plant disease prevention and control, and specifically relates to a combined volatile compound and its application in the prevention and treatment of mango postharvest anthracnose. Background technique [0002] Mango is one of the most important tropical fruits. It is widely planted in tropical and subtropical regions. China is the world's second largest mango producer, mainly in Hainan, Guangxi, Yunnan, Sichuan, Guangdong, Fujian and other provinces. The fungus Colletotrichum Corda is a kind of plant pathogens distributed globally. The mango anthracnose caused by it is one of the most important diseases on mangoes. It forms a latent infection on the immature mangoes and causes The disease in storage and transportation causes huge economic losses. At present, mango anthracnose mainly depends on chemical agents for prevention and control, but the extensive use of chemical agents can easily cause pesticide residues and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N41/12A01N35/04A01N31/04A01N27/00A01N25/18A01P3/00
CPCA01N25/18A01N27/00A01N31/04A01N35/04A01N41/12
Inventor 李其利唐利华莫贱友郭堂勋黄穗萍
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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