Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases
A technology of turfgrass and bacterial strains, applied in the direction of application, fungicides, fungi, etc.
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Embodiment 1
[0030] Example 1 Screening and identification of Trichoderma harzianum (Trichoderma harzianum) SQ-18 bacterial strain
[0031] 1. Experimental method
[0032] The numbers of the 4 Trichoderma strains tested are: SQ-18 (Hainan, Sanya, Shenquan International Golf Course), SQ-1Q-15 (Hainan, Sanya, Shenquan International Golf Course), BD-1F-1 (Hainan, Wanning , Shenzhou Peninsula Golf Course), BD-2Q-12 (Hainan, Wanning, Shenzhou Peninsula Golf Course), the turfgrass pathogen information for testing is shown in Table 1.
[0033] Table 1 Information on pathogenic bacteria of tested turfgrass
[0034]
[0035]
[0036] (1) Activation of bacteria
[0037] Four strains of Trichoderma and 14 strains of turfgrass pathogens stored on slopes at 4°C were inoculated into PDA medium for activation, and placed in a constant temperature incubator at 25°C for 5 days before use.
[0038] (2) Determination of antibacterial activity by confrontation culture method
[0039]Use a marker pen...
Embodiment 2
[0050] The antibacterial effect measurement of embodiment 2 Trichoderma harzianum SQ-18 bacterial strain
[0051] 1. Experimental method
[0052] (1) Antagonism level determination
[0053] The Trichoderma colony completely occupies the entire Petri dish, that is, the occupied area = 100%, and the grade is Ⅰ; 3 / 4<Trichoder colony occupies the area of the Petri dish<1, and the grade is II; 2 / 3<The Trichoderma colony occupies the Petri dish area< 3 / 4, the grade is III; 1 / 3< Trichoderma colony occupies the area of the petri dish <2 / 3, the grade is IV; 0< Trichoderma colony occupies the area of the petri dish <1 / 3, the grade is V; pathogenic bacteria colony occupies the culture The dish area is 100%, and the grade is VI.
[0054] (2) Microscopic observation of antibacterial mechanism
[0055] Under sterile conditions, use a pipette to draw 1 mL of sterile PDA medium and spread it evenly on the glass slide to make a layer of PDA medium film. After the culture medium on th...
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