Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases

A technology of turfgrass and bacterial strains, applied in the direction of application, fungicides, fungi, etc.

Active Publication Date: 2021-01-26
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lawn, as an important green space, plays an irreplaceable role in beautifying the environment, purifying the air, maintaining water and soil, and providing outdoor activities and sports, but turfgrass diseases are important factors that affect the functions and sustainable development of turf in all aspects. At present, the management and control measures of turfgrass diseases, which are still dominated by chemical control methods, are one of the main factors limiting their healthy development.
The biological control of turfgrass diseases is a research hotspot in today's lawn management technology. The advantages of using bacteria to treat diseases lie in rich resources, strong selectivity, no residue, no pollution, low cost, and both prevention and treatment, which play a long-term role in maintaining ecological balance. However, domestic and foreign studies on Trichoderma are relatively few in the prevention and treatment of turfgrass diseases, and there are only reports that Trichoderma harzianum is preventing and treating Fusarium wilt of turf (publication number is CN103952320A, and the publication date is July 30, 2014 Chinese invention patent) and lawn brown spot disease (publication number is CN103952321A, publication date is the application in the Chinese invention patent on July 30, 2014)

Method used

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  • Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases
  • Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases
  • Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening and identification of Trichoderma harzianum (Trichoderma harzianum) SQ-18 bacterial strain

[0031] 1. Experimental method

[0032] The numbers of the 4 Trichoderma strains tested are: SQ-18 (Hainan, Sanya, Shenquan International Golf Course), SQ-1Q-15 (Hainan, Sanya, Shenquan International Golf Course), BD-1F-1 (Hainan, Wanning , Shenzhou Peninsula Golf Course), BD-2Q-12 (Hainan, Wanning, Shenzhou Peninsula Golf Course), the turfgrass pathogen information for testing is shown in Table 1.

[0033] Table 1 Information on pathogenic bacteria of tested turfgrass

[0034]

[0035]

[0036] (1) Activation of bacteria

[0037] Four strains of Trichoderma and 14 strains of turfgrass pathogens stored on slopes at 4°C were inoculated into PDA medium for activation, and placed in a constant temperature incubator at 25°C for 5 days before use.

[0038] (2) Determination of antibacterial activity by confrontation culture method

[0039]Use a marker pen...

Embodiment 2

[0050] The antibacterial effect measurement of embodiment 2 Trichoderma harzianum SQ-18 bacterial strain

[0051] 1. Experimental method

[0052] (1) Antagonism level determination

[0053] The Trichoderma colony completely occupies the entire Petri dish, that is, the occupied area = 100%, and the grade is Ⅰ; 3 / 4<Trichoder colony occupies the area of ​​the Petri dish<1, and the grade is II; 2 / 3<The Trichoderma colony occupies the Petri dish area< 3 / 4, the grade is III; 1 / 3< Trichoderma colony occupies the area of ​​the petri dish <2 / 3, the grade is IV; 0< Trichoderma colony occupies the area of ​​the petri dish <1 / 3, the grade is V; pathogenic bacteria colony occupies the culture The dish area is 100%, and the grade is VI.

[0054] (2) Microscopic observation of antibacterial mechanism

[0055] Under sterile conditions, use a pipette to draw 1 mL of sterile PDA medium and spread it evenly on the glass slide to make a layer of PDA medium film. After the culture medium on th...

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Abstract

The invention discloses a Trichoderma harzianum SQ-18 strain and application thereof to prevention and treatment of turfgrass diseases. The invention provides the Trichoderma harzianum SQ-18 strain which is preserved in Guangdong Microbial Culture Collection Center on July 26, 2018, and the preservation number of the Trichoderma harzianum SQ-18 strain is GDMCC No: 60422. The Trichoderma harzianumSQ-18 strain achieves an obvious inhibition effect on Rhizoctonia zeae, Laetisaria fuciformis, Botrytis cinerea, Colletotrichum hainanese and Rhizoctonia solani; secondary metabolites of the Trichoderma harzianum SQ-18 strain achieve a relatively strong antagonistic effect on Sclerotinia homoeocarpa, Nigrospora sphaerica and Laetisaria fuciformis; and the inhibition rate of a bacterium solution crude extract of the Trichoderma harzianum SQ-18 strain on the Sclerotinia homoeocarpa and the Bipolaris peregianensis is high. Therefore, the Trichoderma harzianum SQ-18 strain or the secondary metabolites or the bacterium solution crude extract of the Trichoderma harzianum SQ-18 strain has wide application prospects in prevention and treatment of diseases caused by turfgrass pathogenic bacteria orpreparation of turfgrass disease prevention and treatment preparations.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a Trichoderma harzianum SQ-18 strain and its application in preventing and treating turfgrass diseases. Background technique [0002] In recent years, the diversity and pathogenicity of plant pathogenic bacteria have shown an increasing trend. The reason is not only related to the extreme weather caused by climate change, but also related to the long-term and extensive use of a large number of herbicides, fungicides and other pesticides, which seriously affects the Plant growth, yield, and environmental and ecological safety. Therefore, the use of antagonistic microorganisms to control the occurrence and damage of plant diseases has become one of the focus and hot spots of current research. [0003] Trichoderma (Trichoderma spp.) is a fungus belonging to the kingdom Fungi, phylum Deuteromycota, class Hyphomycetes, order Hyphospora, family Pleurophycea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCA01N63/38C12R2001/885C12N1/145
Inventor 章武杨锦玉卢翔林金梅牛学礼
Owner LINGNAN NORMAL UNIV
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