Preparation method of anti-atherosclerosis active laminarins

Abstract

The invention relates to a preparation method of anti-atherosclerosis active laminarins,Which comprises the following steps: 1. washing Laminaria digitata; 2. removing water from the Laminaria digitata; 3. preparing Laminaria digitata powder; 4. extracting laminarins; 5. removing alginates; 6. removing proteins; and 7. precipitating the laminarins. The extraction solvent is distilled water instead of acid liquor or alkali liquor in the traditional technique, thereby greatly lowering the equipment corrosion and environmental pollution caused by acid liquor or alkali liquor in the production process; the laminarin component A and the laminarin component B prepared by the method provided by the invention have strong anti-atherosclerosis activity; and compared with the model set, the inhibition rates of the laminarin component A and the laminarin component B for low-density lipoprotein acceptor defect type mouse endarterium lipidosis are respectively up to 75% and 87%, which indicates that water-soluble laminarins are the material basis of Laminaria digitata for displaying anti-atherosclerosis activity.

Description

technical field

[0001] The invention belongs to the technical field of comprehensive processing of marine biological resources, and in particular relates to a preparation method of anti-atherosclerosis active laminarin. Background technique

[0002] kelp( Laminaria japonica ) is an important marine resource that has the functions of "weight loss and detumescence" clearly recorded in the herbal medicines of all dynasties in my country. It has a history of edible and medicinal use for more than 1,500 years. At present, there is a large amount of artificial cultivation of kelp, which is cultivated in various coastal areas of my country, and its output ranks first in the world. Pharmacodynamic studies have shown that kelp is rich in polysaccharides, vitamins, taurine, mannitol and other functional ingredients, among which polysaccharides have been proved to be effective in reducing blood fat, anti-oxidation, blood pressure, blood sugar, radiation and anti-aging. The main activ...

Examples

Embodiment

[0018] Step 1: Washing of kelp: Soak fresh kelp in tap water for 3 days, change the water every 3 hours and wash.

[0019] Step 2: Dehydration of kelp: place the washed kelp in a ventilated drying box, and dry continuously at a temperature of 40° C. for 48 hours.

[0020] Step 3: Preparation of kelp powder: put the above-mentioned dried kelp into a G-125 colloid mill for pulverization to obtain kelp powder with a particle size of ≤200 mesh.

[0021] Step 4: polysaccharide extraction: add distilled water to the dry kelp powder prepared above at a weight-to-volume ratio of 1:50 to obtain a mixture of kelp powder and distilled water, and perform ultrasonication on the mixture of kelp powder and distilled water under the condition of ultrasonic power of 600W After processing for 10 minutes, the mixture A of kelp powder and water was obtained; the mixture A of kelp powder and water was placed on a magnetic stirrer, and stirred and extracted for 3 hours at a rotation speed of 2000 r...

technical field

[0001] The invention belongs to the technical field of comprehensive processing of marine biological resources, and in particular relates to a preparation method of anti-atherosclerosis active laminarin. Background technique

[0002] kelp( Laminaria japonica ) is an important marine resource that has the functions of "weight loss and detumescence" clearly recorded in the herbal medicines of all dynasties in my country. It has a history of edible and medicinal use for more than 1,500 years. At present, there is a large amount of artificial cultivation of kelp, which is cultivated in various coastal areas of my country, and its output ranks first in the world. Pharmacodynamic studies have shown that kelp is rich in polysaccharides, vitamins, taurine, mannitol and other functional ingredients, among which polysaccharides have been proved to be effective in reducing blood fat, anti-oxidation, blood pressure, blood sugar, radiation and anti-aging. The main activ...

Examples

Embodiment

[0018] Step 1: Washing of kelp: Soak fresh kelp in tap water for 3 days, change the water every 3 hours and wash.

[0019] Step 2: Dehydration of kelp: place the washed kelp in a ventilated drying box, and dry continuously at a temperature of 40° C. for 48 hours.

[0020] Step 3: Preparation of kelp powder: put the above-mentioned dried kelp into a G-125 colloid mill for pulverization to obtain kelp powder with a particle size of ≤200 mesh.

[0021] Step 4: polysaccharide extraction: add distilled water to the dry kelp powder prepared above at a weight-to-volume ratio of 1:50 to obtain a mixture of kelp powder and distilled water, and perform ultrasonication on the mixture of kelp powder and distilled water under the condition of ultrasonic power of 600W After processing for 10 minutes, the mixture A of kelp powder and water was obtained; the mixture A of kelp powder and water was placed on a magnetic stirrer, and stirred and extracted for 3 hours at a rotation speed of 2000 r...