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Pollution-free comprehensive prevention and control method for oil camellia anthracnose

A comprehensive technology for camellia oleifera anthracnose, applied in botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as insecurity

Active Publication Date: 2016-09-21
安徽德昌苗木有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, about 60% of vegetable edible oil in our country depends on imports or is controlled by foreign-funded enterprises, which is very unsafe

Method used

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  • Pollution-free comprehensive prevention and control method for oil camellia anthracnose
  • Pollution-free comprehensive prevention and control method for oil camellia anthracnose
  • Pollution-free comprehensive prevention and control method for oil camellia anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A pollution-free comprehensive control method for camellia oleifera anthracnose, characterized in that the steps include:

[0024] (1) Weigh 20g of beef extract and 10g of peptone and dissolve them in 1L of water, and dissolve them by shaking at a constant temperature. The temperature of the water bath is 80°C. High temperature sterilization at ℃, after sterilization, take out the culture solution and transfer it to a petri dish, the volume of the culture solution should account for 0.3 of the volume of the petri dish;

[0025] (2) Cool the above-mentioned culture solution to 20° C., then inoculate the anthrax parent species under aseptic conditions, and the inoculation method is the plate marking method;

[0026] (3) putting the petri dish inoculated with the anthrax parent species into a constant temperature incubator with a set temperature of 25°C for cultivation;

[0027] (4) Configure 12 kinds of medicaments with different concentrations and pour 5ml into the cult...

Embodiment 2

[0039] A pollution-free comprehensive control method for camellia oleifera anthracnose, characterized in that the steps include:

[0040] (1) Weigh 25g of beef extract and 12g of peptone and dissolve them in 1L of water, and dissolve them by shaking at a constant temperature. The temperature of the water bath is 80°C. High temperature sterilization at ℃, after sterilization, take out the culture solution and transfer it to a petri dish, the volume of the culture solution should account for 0.4 of the volume of the petri dish;

[0041] (2) Cool the above-mentioned culture solution to 25° C., then inoculate the anthrax parent species under aseptic conditions, and the inoculation method is the plate marking method;

[0042] (3) putting the petri dish inoculated with the anthrax parent species into a constant temperature incubator with a set temperature of 25°C for cultivation;

[0043] (4) Configure 12 kinds of medicaments with different concentrations and pour 5ml into the cult...

Embodiment 3

[0055] A method for researching anthracnose pollution-free prevention and control technology, is characterized in that, its step comprises:

[0056] (1) Weigh 30g of beef extract and 15g of peptone and dissolve them in 1L of water, and dissolve them by shaking at a constant temperature. The temperature of the water bath is 80°C. High temperature sterilization at ℃, after sterilization, take out the culture solution and transfer it to a petri dish, the volume of the culture solution should account for 0.5 of the volume of the petri dish;

[0057] (2) Cool the above-mentioned culture solution to 30° C., and then inoculate the anthrax parent species under aseptic conditions, and the inoculation method is the plate marking method;

[0058](3) putting the petri dish inoculated with the anthrax parent species into a constant temperature incubator with a set temperature of 25°C for cultivation;

[0059] (4) Configure 12 kinds of medicaments with different concentrations and pour 5ml...

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Abstract

The invention discloses a pollution-free comprehensive prevention and control method for oil camellia anthracnose. The method includes the steps that 1, beef extract peptone is dissolved in a constant-temperature oscillation mode, wherein the water bath temperature is 80 DEG C, and due to the fact that the two substances are hard to dissolve, preparation of a culture solution can be accelerated in a heating oscillation mode; 2, the pH of the culture solution obtained in the step 1 is adjusted, the pH of the culture solution is adjusted to be 5.6, the pH is most suitable for growth of an anthrax strain, and the strain grows smoothly, wherein the inoculation mode of the strain obtained in the step 2 is a plate streak method, an inoculation method includes the plate streak method and a reverse plate method, due to the fact that the reverse plate method is prone to strain stacking to affect the experimental result, the plate streak method is adopted as a preferred method for strain inoculation, the culture temperature of the strain in the step 3 is 25 DEG C, the amount of agent poured every time in the step 4 is 5 ml, and in order to avoid the problems that the strain is killed at a time when too much agent is poured, and the effect is not obvious when too little agent is poured, and the dosage is adopted for the strain to be fed into medicine.

Description

technical field [0001] The invention relates to the technical field of germ prevention and control, in particular to a pollution-free comprehensive prevention and treatment method of camellia oleifera anthracnose. Background technique [0002] Camellia oleifera is a woody oil tree species, and edible oil safety is another new topic related to national strategy after global oil safety and food safety. At present, about 60% of vegetable edible oil in our country depends on imports or is controlled by foreign-funded enterprises, which is very unsafe. In recent years, the state has begun to pay great attention to the safety of edible oil. The State Council held a special on-site meeting on the development of camellia oleifera industry in Hunan in 2008; from 2009 to 2010, the No. 1 document of the central government mentioned twice that it is necessary to vigorously develop woody oil species such as camellia oleifera. It can be seen that The importance and urgency of developing ...

Claims

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Application Information

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IPC IPC(8): C12Q1/18A01G13/00
CPCA01G13/00C12Q1/18
Inventor 方先红詹文勇詹秀芝周玉环朱小妹詹志彬夏挺王永友杨雪梅方华桥
Owner 安徽德昌苗木有限公司
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