A strain of Trichoderma and its application in agricultural field

A trichoderma and agricultural technology, applied in the field of bioengineering, can solve the problems of large fluctuations in effect, limited application range, single source, etc., to reduce risks and human maintenance costs, reduce the use of chemical fertilizers and pesticides, and reduce the application of chemical fertilizers Effect

Active Publication Date: 2021-03-16
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the biocontrol strains of plant diseases are fungi and bacteria isolated from the soil and the rhizosphere or body surface of plants. Their sources are relatively single and they are easily affected by the external natural environment.
If the environment changes, it is easy to directly affect its colonization ability, and the effect fluctuates greatly when facing different harmful bacteria. It is difficult to prevent and control most diseases, which seriously limits its application range.

Method used

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  • A strain of Trichoderma and its application in agricultural field
  • A strain of Trichoderma and its application in agricultural field
  • A strain of Trichoderma and its application in agricultural field

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0036] 1. Isolation and screening of Trichoderma harzianum strains

[0037] The samples come from soil samples collected in Wuzhi Mountain, Hainan Province.

[0038] Weigh 5g of Hainan sample soil, add it to 45mL of sterilized Tween water with a concentration of 0.1%, oscillate for 1 minute, mix well and let it stand for 25 minutes, take the soil liquid and dilute it to 10 -3 、10 -4 Concentration, and then coated in the PDA solid inorganic salt medium, and cultured upside down in a 28°C constant temperature incubator for 4 days. Inverted culture in medium for 5 days, then observe the colony type, and observe the cell type with an optical microscope (1000X). The schematic diagram of the colony type is as figure 1 shown. The colony is round, radial, and the aerial hyphae are unevenly distributed, and the surface is white to yellow-green flocculent.

[0039] Subsequently, the ITS sequence of the strain was identified as species, and the determination result was shown in the ...

specific Embodiment 2

[0122] Roughly the same as specific embodiment 1, the difference only lies in:

[0123] Sodium alginate was used as a water-soluble film-forming agent and xanthan gum was used as an adhesive in the preparation process of the microbial seed coating agent.

specific Embodiment 3

[0125] Roughly the same as specific embodiment 1, the difference only lies in:

[0126] In the preparation process of the microbial seed coating agent, polyvinylamide is used as a water-soluble film-forming agent, and carboxy cellulose or aluminum magnesium silicate is used as an adhesive.

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Abstract

The invention relates to a Trichoderma strain and its application in the agricultural field. A strain of Trichoderma, named Trichoderma harzianum, was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.15679 and the preservation date was May 21, 2018. The above-mentioned Trichoderma is used to inhibit the growth of Rhizoctonia solani, Anthracnose and Fusarium. The above-mentioned Trichoderma is used for soil phosphorus solubilization. The above-mentioned Trichoderma is used for preparing microbial fertilizer. A strain of Trichoderma disclosed by the present invention and its application in the agricultural field have the following beneficial effects: 1. Provide a bacterial strain for inhibiting Rhizoctonia solani, anthracnose, and Fusarium, which has a good effect on the above-mentioned plant harmful pathogens 2. The bacterial strain can also be used to prepare microbial seed coating agents, which can play a beneficial role in promoting the growth of plant roots without losing activity.

Description

technical field [0001] The invention belongs to the field of biological engineering, and in particular relates to a Trichoderma strain and its application in the agricultural field. Background technique [0002] Worldwide, plant diseases commonly cause severe losses to agricultural production. The control of plant diseases and insect pests in production is often based on traditional chemical control, which has high requirements for the long-term stability of the chemical agents, and the chemical agents will also cause serious pollution to the environment. After pesticides are applied to crops, once they remain, it will greatly affect the quality of agricultural products and seriously endanger human health. With the strengthening of human awareness of environmental protection, the development of green ecological agriculture has gradually become the mainstream, especially in the use of biological methods instead of traditional chemical methods for the prevention and control o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/885
CPCC05F11/08C12N1/145C12R2001/885
Inventor 王科晶邹秋霞金漫蒋先芝
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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