A strain of Trichoderma and its application in agricultural field
A trichoderma and agricultural technology, applied in the field of bioengineering, can solve the problems of large fluctuations in effect, limited application range, single source, etc., to reduce risks and human maintenance costs, reduce the use of chemical fertilizers and pesticides, and reduce the application of chemical fertilizers Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment 1
[0036] 1. Isolation and screening of Trichoderma harzianum strains
[0037] The samples come from soil samples collected in Wuzhi Mountain, Hainan Province.
[0038] Weigh 5g of Hainan sample soil, add it to 45mL of sterilized Tween water with a concentration of 0.1%, oscillate for 1 minute, mix well and let it stand for 25 minutes, take the soil liquid and dilute it to 10 -3 、10 -4 Concentration, and then coated in the PDA solid inorganic salt medium, and cultured upside down in a 28°C constant temperature incubator for 4 days. Inverted culture in medium for 5 days, then observe the colony type, and observe the cell type with an optical microscope (1000X). The schematic diagram of the colony type is as figure 1 shown. The colony is round, radial, and the aerial hyphae are unevenly distributed, and the surface is white to yellow-green flocculent.
[0039] Subsequently, the ITS sequence of the strain was identified as species, and the determination result was shown in the ...
specific Embodiment 2
[0122] Roughly the same as specific embodiment 1, the difference only lies in:
[0123] Sodium alginate was used as a water-soluble film-forming agent and xanthan gum was used as an adhesive in the preparation process of the microbial seed coating agent.
specific Embodiment 3
[0125] Roughly the same as specific embodiment 1, the difference only lies in:
[0126] In the preparation process of the microbial seed coating agent, polyvinylamide is used as a water-soluble film-forming agent, and carboxy cellulose or aluminum magnesium silicate is used as an adhesive.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com