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Antagonistic bacterium capable of increasing pH value of acid soil and preparation and application of microbial agent of antagonistic bacterium

A technology of inoculum and bacillus is applied in the field of preparation of antagonistic bacteria and inoculants, which can solve problems such as affecting the normal growth and development of plant roots, improve plant rhizosphere microbial community structure, promote plant growth, and achieve low cost of raw materials. Effect

Active Publication Date: 2020-05-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although different crops have different tolerances to soil pH, soil acidification still significantly affects the normal growth and development of plant roots

Method used

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  • Antagonistic bacterium capable of increasing pH value of acid soil and preparation and application of microbial agent of antagonistic bacterium
  • Antagonistic bacterium capable of increasing pH value of acid soil and preparation and application of microbial agent of antagonistic bacterium
  • Antagonistic bacterium capable of increasing pH value of acid soil and preparation and application of microbial agent of antagonistic bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: Isolation and screening of bacterial strain

[0054] Collect soil samples from Taishan forest land in Tai'an City, Shandong Province, take 10g of soil samples under aseptic conditions, dissolve in 90mL sterile distilled water, 220r / min, vibrate for 30min, absorb 1mL and mix with 9mL sterile water to obtain 10 -1 diluent, then take 1mL 10 -1 The diluted solution was mixed with 9mL sterile water to obtain 10 -2 Dilutions, indistinctly, to prepare 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 Wait for a series of dilutions. The chosen dilution is 10 -5 、10 -6 、10 -7 100uL of the soil dilution solution was drawn and spread on the PDA medium, and cultured at a constant temperature of 30-32°C for 1-3 days. Single colonies were picked and streaked into pure cultures. Under sterile conditions, scrape two loops of pure culture and inoculate them into LB liquid medium, culture with shaking at 200 r / min at 30°C for 8-10 hours, and inoculate into LB liquid medium w...

Embodiment 2

[0056] Example 2: Identification of IV-4 strain

[0057] (1) Cell morphology and colony characteristics

[0058] The colony of strain IV-4 on the PDA plate is milky white, round and translucent, with neat edges, dry surface, slightly raised in the middle of the colony, and the thalline morphology observed under a microscope is rod-shaped, Gram-positive bacteria.

[0059] (2) Physiological and biochemical characteristics

[0060] The physiological and biochemical characteristics of strain IV-4 are shown in Table 1. The aerobic nature of the strain is facultative anaerobic, Gram reaction, glucose oxidation fermentation test, contact enzyme reaction, starch hydrolysis test, methyl red test, salt tolerance test, phenylalanine amino acid deaminase test, nitrate reduction and citrate utilization etc. are positive; V-P test is negative; it can grow in LB liquid medium with NaCl concentration of 2% and 5%.

[0061] Table 1: Physiological and biochemical characteristics of strain IV...

Embodiment 3

[0077] Embodiment 3: the bacteriostatic spectrum determination of IV-4 bacterial strain

[0078] Use an inoculation loop to pick a ring of purified IV-4 thallus, and point it at the two ends of the PDA plate at a distance of 3 cm from the center of the PDA plate. Alternaria and poplar canker bacterium were used as targets, and bacteria cakes with a diameter of 5 mm were cut out with a puncher and inoculated in the center of a PDA plate, and cultured at 28°C for 2 to 3 days. For the antibacterial spectrum of strain IV-4, see image 3 .

[0079] The test results showed that the IV-4 strain had good antagonistic effects on Fusarium oxysporum, Helminthromyces oxysporum, Fusarium graminearum, Colloidal anthracnose, Alternaria and Poplar canker.

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Abstract

The invention discloses an antagonistic bacterium capable of increasing the pH value of acid soil and preparation and application of a microbial agent of the antagonistic bacterium. The antagonistic bacterium is bacillus amyloliquefaciens and is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO. 18619. The antagonistic bacterium has astrong antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium graminearum, colletotrichum gloeosporioides, alternaria alternata and botryosphaeria dothidea, can be stably colonized at rhizosphere of plants such as wheat, corn, pepper and poplar, and can remarkably increase the pH value of the acid soil and promote crop growth. The microbial agent prepared from the antagonistic bacterium can be applied to improvement of growth of plants such as wheat, corn, pepper and poplar in the acid soil and prevention and control of fungal diseases, and has effects of increasing the pH value of the acid soil, preventing diseases and promoting growth; and the preparation process of the microbial agent is simple, the fermentation period is short, the cost islow, and industrial production and transportation are facilitated.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to the preparation and application of an antagonistic bacterium capable of increasing the pH value of acidic soil and its bacterial agent. Background technique [0002] Due to the H in the soil solution + and OH - Soils that have an ion imbalance such that the soil pH is less than 6.5 are called acidic soils. Acidic soil is mainly distributed in most areas outside the cold zone of the world, and its natural causes are closely related to climate. Because protons in soil participate in almost all chemical reaction cycles, soil acidification will cause the balance of protons to be broken, thereby affecting the transformation of soil element forms. Generally speaking, the pH of the soil suitable for most crops is between slightly acidic and neutral. Although different crops have different tolerances to soil pH, soil acidification still significantly affects the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/22A01P3/00C05F11/08C12R1/07
CPCC12N1/20C09K17/14A01N63/00C05F11/08C12R2001/07C12N1/205
Inventor 刘训理宋鑫纪超王长栋刘玥郝立平杜建峰周艳艳徐睿娉李虎滢
Owner SHANDONG AGRICULTURAL UNIVERSITY
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