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Application of fructus caryophylli volatile oil

A technology of volatile oil and female clove, applied in the direction of application, chemicals for biological control, fungicides, etc., to achieve high efficiency, long-lasting control effect, and quality improvement effect

Active Publication Date: 2019-03-12
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no document disclosure related to the technical solution of the present invention

Method used

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  • Application of fructus caryophylli volatile oil
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  • Application of fructus caryophylli volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of female clove volatile oil

[0021] (1) Add 8 times the mass of water to 1 kg of crushed mother clove powder, soak for 2 hours, use steam to extract the volatile oil in mother cloves by means of heating and distillation, the extraction time is 6 hours, and condense to collect the effluent;

[0022] (2) Dry the collected volatile oil with anhydrous sodium sulfate to remove the contained water, put it into a brown sample bottle, seal it, and store it at -20°C until use.

Embodiment 2

[0023] Embodiment 2: the activation of pathogenic bacteria

[0024] (1) The pathogenic bacteria isolated from the roots and shoots of Panax notoginseng were purified and identified as Fusarium oxysporum ( Fusarium oxysporum ), Fusarium solani ( Fusarium solani ), Pythium ( Pythium aphanidermatum ), Cylindrosporium destroya ( Cylindrocarpon destructans ), Botrytis ( Botrytis cinerea ), Glosporum anthracnose ( Colletotrichum gloeosporioides ) and Rhizoctonia solani ( Rhizoctonia solani ) strains were inoculated on PDA medium for activation, and after inoculation 4 times, the vigorously growing strains were taken for use;

[0025] (2) Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water.

Embodiment 3

[0026] Embodiment 3: GC-MS measures the chemical composition of mother clove volatile oil

[0027] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS 30m × 250μm × 0.25μm;

[0028] (2) EI source Ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30~500m / z; inlet temperature: 285°C; split ratio 10:1; injection volume: 1 μl; Electron energy: 70eV; column oven temperature rise program: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the continuous carbon (C9-C25) of alkanes is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;

[0029]

[0030] In the table: a: retention time; b: relative peak area; ± relative deviation; c: retention index.

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PUM

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Abstract

The invention discloses application of fructus caryophylli volatile oil, namely, application of fructus caryophylli volatile oil to prevention of traditional Chinese medicinal herbe or crop root rot in the agriculture field or application to preventing and treating pathogenic bacteria which cause disease of the traditional Chinese medicinal herbse or the crops above the ground. The experimental result shows that fructus caryophylli volatile oil can inhibit and damage growth of hyphaeropes of 4 kinds of main pathogenic bacteria including fusarium oxysporum, fusarium solani, pythium and cylindrocarpon which cause occurrence of root rot of radix notoginseng, and can completely inhibit growth of hyphaeropes of 3 kinds of pathogenic bacteria including botrytis cinerea, colletotrichum dematium and rhizoctonia solani which cause occurrence of diseases of radix notoginseng above the ground. The antibacterial effect of the fructus caryophylli volatile oil is comparable to that of a chemical pesticide hymexazol, and fructus caryophylli and hymexazol have the synergistic effect on a plurality of bacterial strains when being compounded. The volatile oil has the features of being fragrant and capable of avoiding dirtiness because of the effumability of the volatile oil, therefore the fertilizer or the pesticide which isare developed based on the fructus caryophylli volatile oil will have the advantages of being safe, and effective and low residue.

Description

technical field [0001] The invention relates to the application of the volatile oil of mother clove, in particular to the control effect of the volatile oil of mother clove on root rot of Chinese medicinal materials or crops and various diseases of the aboveground parts of Chinese medicinal materials or crops, and belongs to the technical field of biopesticides. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, is a perennial herb of Araliaceae ginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain. It is a rare and precious cultivated medicinal plant unique to my country. , which has a cultivation history of more than 400 years in Wenshan Prefecture, Yunnan Province. The roots, stems, leaves, and flowers of Panax notoginseng can be used as medicine. The range of medicines is very wide, and the social demand is constantly increasing. With the rapid increase of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/28A01P3/00
CPCA01N65/00A01N65/28
Inventor 董鲜程永现徐福荣马玉楠
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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