A method for inducing anthrax bacteria to produce conidia

A technology for conidia and anthrax, which is applied in the methods of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of ineffective methods of inducing spores by anthrax, and achieves a large amount of spores and a Stable, convenient and fast drying, and the effect of huge spore production

Active Publication Date: 2022-02-25
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Aiming at the problem that the existing anthrax bacteria has no effective method for inducing the production of spores, the present invention provides a method for inducing the production of conidia in the anthrax bacteria, which can effectively induce the mass production of anthrax spores

Method used

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  • A method for inducing anthrax bacteria to produce conidia
  • A method for inducing anthrax bacteria to produce conidia
  • A method for inducing anthrax bacteria to produce conidia

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Embodiment 1

[0044] A method for inducing anthrax bacteria to produce conidia, comprising the steps of:

[0045] (1) Activation of G. anthracnose: On a clean bench, inoculate PDA plate culture medium with G. anthracis (strain purchased from China Agricultural Microorganism Culture Collection Management Center, strain number: ACCC 31200) preserved at 4°C The activated culture is carried out on the above-mentioned activated culture, and the condition of the described activated culture is to cultivate in the dark at a temperature of 27° C. for 5 days to obtain the activated G. anthracnose;

[0046] (2) Naturally induce spore production (natural sporulation method): get the activated glyosporium anthracis in step (1) and put it into a constant temperature incubator for constant temperature cultivation. The specific steps are as follows: on the ultra-clean workbench, use a diameter of A 0.5cm hole puncher is punched at the colony edge of the PDA plate culture medium, and the mycelia block (i.e....

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Abstract

The invention discloses a method for inducing the production of conidia of anthrax bacteria, which belongs to the technical field of plant protection; it comprises the following steps: (1) activation of anthrax bacteria: inoculating anthrax bacteria on a culture medium for activation and culture, and obtaining the activated (2) Inducing spore production: take the activated anthrax bacteria in step (1) and put them into an oven for high-temperature culture, then take them out and place them in a constant temperature incubator at 27°C for constant temperature culture for 7 days, then the conidia can be induced Produce; the present invention carries out high-temperature culture first and then carries out constant temperature culture to anthrax bacteria first, realizes simple and large amount of induction anthrax bacteria to produce a large number of spores, for the pathogenicity determination of anthrax bacteria, its bactericide bioactivity determination and the identification of disease resistance of varieties etc. research is of great significance.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for inducing anthracnose bacteria to produce conidia. Background technique [0002] Crop anthracnose is a fungal disease caused by anthracnose bacteria (Colletotrichum sp.). In addition to infecting field crops, it can also damage various fruit trees and flowers such as figs, peaches, pears, grapes and citrus. With climate change, the disease is becoming more and more serious, becoming the main disease affecting crop yield and quality. In order to be used in studies such as the determination of the pathogenicity of anthrax bacteria, the determination of the biological activity of fungicides, and the identification of disease resistance of varieties, the conventional methods for inducing spore production of anthrax bacteria are the natural sporulation method and the yeast PDA induction method: [0003] Natural sporulation method: pick mycelium preser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N1/14C12N3/00
Inventor 檀根甲李丹丹汪章勋叶磊刘灿
Owner ANHUI AGRICULTURAL UNIVERSITY
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