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Application of origanum vulgare essential oil in prevention and treatment of botanical pathogens

A technology for plant diseases and Sclerotinia sclerotiorum, applied in the fields of application, plant growth regulators, botanical equipment and methods, etc., can solve problems such as soil pollution, chemical residues, and economic losses of farmers, and achieve high bactericidal activity and strong inhibition The effect of broad-spectrum bactericidal effect

Inactive Publication Date: 2021-01-15
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant diseases have brought huge economic losses to farmers and seriously affected the quality and yield of plants
Chemical agents have always been the main effective means to prevent and control plant diseases, but long-term use of chemical agents will not only produce chemical residues, cause soil pollution, but also cause plant drug resistance, and even destroy the ecological balance

Method used

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  • Application of origanum vulgare essential oil in prevention and treatment of botanical pathogens
  • Application of origanum vulgare essential oil in prevention and treatment of botanical pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: oregano essential oil is extracted by steam distillation. Take 40g of sample powder and place it in a 1000mL round bottom flask, add 400mL of distilled water, add zeolite, connect the volatile oil extractor, heat, keep a slight boil (control the electric heating mantle voltage around 100 V), distill for 6h until the amount of volatile oil does not increase any more , The volatile oil was extracted several times with distilled diethyl ether. Take the upper oil phase and place it in a stoppered test tube, dry it over anhydrous sodium sulfate, filter, transfer the filtrate into a concentrating bottle, and concentrate it by atmospheric distillation until there is no ether smell to obtain a volatile oil, which is set aside.

Embodiment 2

[0012] Embodiment 2: Determination of indoor antibacterial activity of phytopathogenic fungi and results

[0013] The phytopathogenic fungi used in this experiment were strains preserved at 4°C in the laboratory. Mycelia growth method was adopted. The medium used for all phytopathogenic fungi in the laboratory except Aspergillus flavus was potato agar dextrose medium (referred to as PDA). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.

[0014] Preparation method of PDA medium: Wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter with four layers of gauze and fill the water in a beaker To 1000mL, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, stir to make it fully dissolved, then divide into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool down for later use.

[0015...

Embodiment 3

[0027] Embodiment 3: Determination of indoor antibacterial activity of plant pathogenic bacteria and results

[0028] The phytopathogenic bacteria used in this experiment were stored at -80°C in the laboratory, and the MIC value was determined by the double dilution method. The phytopathogenic bacteria used in the laboratory all use nutrient broth medium (referred to as MH). MH medium formula: MH broth powder 21g, distilled water 1000mL, natural pH.

[0029] MH medium preparation formula: Weigh 21g of MH broth powder into 1000mL of distilled water, stir to dissolve it fully, then divide it into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool down for later use.

[0030] 1) Use the double dilution method.

[0031] 1. Add 100 μL of MH broth medium to each well of a 96-well plate.

[0032] 2. Add 100 μL of the prepared drug solution to the first well of the three rows A / B / C, and then dilute the drug twice. That is, after adding the drug solution to the first we...

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Abstract

The invention belongs to the field of natural pharmaceutical chemistry and discloses an application for preventing and treating diseases caused by corn leave penicillium zeae, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, watermelon gummy stem blight, fusarium oxysporum, magnaporthe grisea, phytophthora capsici, colletotrichum gloeosporioides, fusarium solani, alternaria tenuissima, citrus canker bacteria and potato black shank bacteria. Test results show that the origanum vulgare essential oil has wide bactericidal activity, especially has an excellentinhibition effect on rhizoctonia solani, and can be used for preparing a natural source bactericide.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and discloses a new application of oregano essential oil, in particular to its use in preventing and treating diseases caused by point mold corn leaf, rhizoctonia solani, rape sclerotinia, tomato cinerea, wheat scab, watermelon Phytophthora solani, Fusarium wilt of cotton, Magnaporthe oryzae, Phytophthora capsici, Apple anthracnose, Fusarium oxysporum, Fusarium oatum, Fusarium equisetum, Anthracnose oxysporum, Fusarium solani, Alternaria adenaria , Canker sorrel citrus and Blackleg potato diseases. Background technique [0002] Plant diseases have brought huge economic losses to farmers and seriously affected the quality and yield of plants. Chemical agents have always been the main effective means to prevent and control plant diseases, but long-term use of chemical agents will not only produce chemical residues, cause soil pollution, but also cause plant drug resistance, and even dest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/22A01P3/00A01P1/00
CPCA01N65/22
Inventor 刘映前吴天琳张智军贺颖慧杜莎莎赵文斌李虎马醌原
Owner LANZHOU UNIVERSITY
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