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Tetraploid chemical induction method applicable to short-necked clams

A Philippine clam, chemical induction technology, applied in the fields of application, climate change adaptation, fish farming, etc., can solve the problems of failure to form mature tetraploid induction technology of Philippine clam, improper control of conditions, low induction rate, etc. Achieve the effects of increasing the tetraploid induction rate, prolonging the treatment time, and improving synchronization

Inactive Publication Date: 2016-10-12
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been researches on tetraploid induction of Philippine clam abroad, but the above conditions are not properly grasped, resulting in a low and unstable induction rate, and a mature tetraploid induction technology of Philippine clam has not been formed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) At the beginning of June 2013, at a base of the Hebei Academy of Ocean and Fishery Sciences, 2-year-old clams caught in a tidal flat were used as parent shells. The shell length of the parent shells was 41.1±3.2mm, and the ripening was carried out indoors for 30 days , the gonads are fully mature;

[0033] (2) The water body in the spawning tank is 50L, and the seawater is sand-filtered seawater that has been irradiated with ultraviolet rays for 30 minutes. The parent shellfish is dried in the shade for 4 hours and placed in the bucket, and intermittently inflated. After being selected and washed repeatedly with fresh water, place them individually in a beaker filled with 500mL of seawater. After about 10 minutes, the gametes are basically discharged, and 11 cups of egg fluid and 12 cups of semen are obtained;

[0034] (3) Microscopically inspect each cup of egg liquid, select 3 cups of egg liquid with complete egg membrane, consistent specifications, regular spheri...

Embodiment 2

[0042] (1) At the end of May 2015, in a shellfish seed breeding farm in Qingdao, 2-year-old clams collected in a local pond were used as broods. After about a week of strengthening, the gonads are fully mature;

[0043] (2) The water body in the oxytocin bucket is 30L, and the seawater is sand-filtered seawater irradiated with ultraviolet rays for 50 minutes. The brockfish is dried in the shade for 4 hours and then put into the bucket. The air is intermittently inflated for 1 minute every 1 minute. After 6 minutes, the brockfish starts to discharge gametes. The broodstock that started to discharge gametes was selected, washed repeatedly with fresh water, and placed in a beaker filled with 500mL sand-filtered seawater. After about 8 minutes, the gametes were basically discharged, and 8 cups of egg liquid and 5 cups of semen were obtained;

[0044](3) Microscopically inspect each cup of egg liquid, select 2 cups of egg liquid with complete egg membrane, consistent specifications...

Embodiment 3

[0052] (1) At the beginning of June 2015, in a key laboratory of aquatic ecology in Tianjin, 3-year-old clams collected in the shallow sea of ​​Bohai Bay were used as broods. The shell length of the broods was 43.2±3.2mm, and the gonads matured naturally;

[0053] (2) The water body in the spawning bucket is 30L, and the seawater is sand-filtered seawater irradiated with ultraviolet rays for 30 minutes. The broccoli are dried in the shade for 4 hours and then put into the bucket. The air is intermittently inflated for 1 minute every 1 minute. After 8 minutes, the brockfish starts to discharge gametes. Select the parent shellfish that started to discharge gametes, rinse them repeatedly with fresh water, and place them individually in a beaker filled with 500mL of sand-filtered seawater. After about 8 minutes, the gametes are basically discharged, and 12 cups of egg fluid and 4 cups of semen are obtained;

[0054] (3) Microscopically inspect each cup of egg liquid, select 4 cups ...

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Abstract

The invention relates to a tetraploid chemical induction method suitable for clam philippines. The main content of the method includes: fully promoting the ripening of clams of philippines; Fertilized eggs with high concentration were obtained by artificial insemination; 13 minutes after fertilization, cytochalasin CB was added to treat at a concentration of 0.5 mg / L to inhibit the release of the first polar body; 19 minutes after fertilization, the eggs were washed to remove CB; The fertilized eggs were developed under suitable conditions, and tetraploid Philippine clam embryos were obtained; the seawater used in the processing and development process was sand-filtered seawater, with a salinity of 24-26 and a temperature of 24.8-25°C. The invention utilizes the best sperm and eggs, effectively improves the developmental synchronization of fertilized eggs, significantly improves the tetraploid induction rate, and the induction rate detected by flow cytometry is stable at over 50%.

Description

technical field [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine biotechnology, relates to cell biology, developmental biology and chromosome engineering, in particular to a tetraploid chemical induction method suitable for Philippine clams. Background technique [0002] Philippine clam Ruditapes philippenarum, commonly known as flower clam and clam, belongs to bivalves, clam family, clam genus, is a wide-temperature and wide-half species, and is an important economic shellfish in my country. In 2014, clam production accounted for about 1 / 3 of seawater shellfish production, and it is the shellfish with the highest monoculture production in my country, and its industrial status is very important. At present, wild seedlings are mainly used in breeding, resulting in miniaturization of individuals, poor stress resistance, high mortality in the breeding season, and reduced unit yield. [0003] Triploid shellfish have excellent econ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李永仁梁健郭永军邢克智
Owner TIANJIN AGRICULTURE COLLEGE
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