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A method for constructing a mouse model of neural tube defects

A neural tube deformity and mouse model technology, applied in the field of applied basic medical research, can solve the problems of inability to simulate low folic acid state, low neural tube deformity rate, and high embryonic lethality, and achieve simple methods, small toxic side effects, and embryonic lethality. low rate effect

Active Publication Date: 2021-11-26
首都儿科研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects that the antifolate drug-induced mice in the prior art cannot simulate the low folic acid state in the human physiological environment, and the rate of neural tube defects is low, the embryo lethality rate is high, and the fetal absorption rate is high. , so as to provide a method for constructing a neural tube defect mouse model. The constructed neural tube defect mouse model can simulate the low folic acid state in the human physiological environment, and has a high rate of neural tube defects, a low embryonic lethality rate, and a low fetal absorption rate.

Method used

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  • A method for constructing a mouse model of neural tube defects
  • A method for constructing a mouse model of neural tube defects
  • A method for constructing a mouse model of neural tube defects

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Experimental animals and materials

[0033] SPF grade adult C57BL / 6J mice, male and female, 7-8 weeks old, weighing 17-19 g. Purchased from Beijing Jinmuyang Experimental Animal Breeding Co., Ltd. Methotrexate (MTX) was purchased from Pfizer (Perth) Pty Limited, and the concentration of the stock solution was 500mg / 20mL. The low-folate feed formula is shown in Table 1.

[0034] Table 1 Low folic acid feed formula

[0035]

[0036]

[0037] 2. Experimental equipment

[0038]Surgical instruments, AL140 electronic balance, ES electronic balance, Milli-Q Academic ultrapure water instrument, SW-OJ-1FD medical ultra-clean workbench, low-temperature refrigerator, ultra-low temperature refrigerator, 752-type ultraviolet grating spectrophotometer, centrifuge, Thermo Scientific Vasioskan Flash Spectral Scanning Multimode Reader, Leica RM2255 Automatic Microtome, Leica HI1210 Spreader, Leica HI1220 Dryer, Leica BOND-Max Automatic Immunohistochemical Staining Machine, ...

Embodiment 2

[0048] 1. Experimental animals and materials

[0049] SPF grade adult C57BL / 6J mice, male and female, 7-8 weeks old, weighing 17-19 g. Purchased from Beijing Jinmuyang Experimental Animal Breeding Co., Ltd. Itatresa was purchased from Pfizer (Perth) Pty Limited. The low-folate feed formula is shown in Table 1.

[0050] Table 3 Low folic acid feed formula

[0051]

[0052]

[0053] 2. Experimental equipment

[0054] With embodiment 1.

[0055] 3. Model preparation

[0056] The experimental group was given intraperitoneal injection of itatrexate on day 7.5, and the others were the same as in Example 1.

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Abstract

The invention relates to the field of applied basic medical research, in particular to a method for constructing a neural tube defect mouse model. The present invention uses physical or chemical teratogenic factors to intervene pregnant mice fed with a low folic acid diet, uses low folic acid as a sensitizing factor, imitates the human physiological environment, induces neural tube defects in mice, and greatly increases the success rate of model construction; The method adopted in the invention is simple, has good repeatability, high deformity rate, little toxic and side effects in pregnant mice, and low embryo lethality rate.

Description

technical field [0001] The invention relates to the field of applied basic medical research, in particular to a method for constructing a neural tube defect mouse model. Background technique [0002] Neural tube defects (NTDs) is a high-incidence birth defect disease. It is the type with the highest morbidity and mortality among birth defects. It is also the most common and serious birth defect disease that affects the quality of the population in my country and the world. , NTDs malformation rate in newborns is as high as 0.1-0.5%. NTDs are deformities from the head to the spine due to neural tube failure or insufficiency, ranging from anencephaly to mild spina bifida: including anencephaly, deencephaly, microcephaly, macrocephaly, and hydrocephalus , meningocele, craniospina bifida, and spina bifida. NTDs usually occur in the early stages of pregnancy, and once irreversible, most of them die during pregnancy or within a short period of time after birth. The occurrence an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/519A61K31/505A61P25/00
CPCA61K31/505A61K31/519A61P25/00
Inventor 裴培王珊张霆崔小岱
Owner 首都儿科研究所
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