Method for constructing a neural tube deformity mouse model

A neural tube defect and mouse model technology, applied in the field of applied basic medical research, can solve the problems of inability to simulate the low folic acid state, low rate of neural tube defects, high embryonic lethality, etc., with simple methods, less toxic side effects, and embryonic lethality low rate effect

Active Publication Date: 2019-02-15
首都儿科研究所
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  • Claims
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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects that the antifolate drug-induced mice in the prior art cannot simulate the low folic acid state in the human physiological environment, and the rate of neural tube defects is low, the embryo lethality rate is high, and the fetal absorption rate is high. , so as to provide a method for constructing a neural tube defect mouse model. The constructed neural tube defect mouse model can simulate the low folic acid state in the human physiological environment, and has a high rate of neural tube defects, a low embryonic lethality rate, and a low fetal absorption rate.

Method used

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  • Method for constructing a neural tube deformity mouse model
  • Method for constructing a neural tube deformity mouse model
  • Method for constructing a neural tube deformity mouse model

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Experimental animals and materials

[0033] SPF grade adult C57BL / 6J mice, male and female, 7-8 weeks old, weighing 17-19 g. Purchased from Beijing Jinmuyang Experimental Animal Breeding Co., Ltd. Methotrexate (MTX) was purchased from Pfizer (Perth) Pty Limited, and the concentration of the stock solution was 500mg / 20mL. The low-folate feed formula is shown in Table 1.

[0034] Table 1 Low folic acid feed formula

[0035]

[0036]

[0037] 2. Experimental equipment

[0038]Surgical instruments, AL140 electronic balance, ES electronic balance, Milli-Q Academic ultrapure water instrument, SW-OJ-1FD medical ultra-clean workbench, low-temperature refrigerator, ultra-low temperature refrigerator, 752-type ultraviolet grating spectrophotometer, centrifuge, Thermo Scientific Vasioskan Flash Spectral Scanning Multimode Reader, Leica RM2255 Automatic Microtome, Leica HI1210 Spreader, Leica HI1220 Dryer, Leica BOND-Max Automatic Immunohistochemical Staining Machine, ...

Embodiment 2

[0048] 1. Experimental animals and materials

[0049] SPF grade adult C57BL / 6J mice, male and female, 7-8 weeks old, weighing 17-19 g. Purchased from Beijing Jinmuyang Experimental Animal Breeding Co., Ltd. Itatresa was purchased from Pfizer (Perth) Pty Limited. The low-folate feed formula is shown in Table 1.

[0050] Table 3 Low folic acid feed formula

[0051]

[0052]

[0053] 2. Experimental equipment

[0054] With embodiment 1.

[0055] 3. Model preparation

[0056] The experimental group was given intraperitoneal injection of itatrexate on day 7.5, and the others were the same as in Example 1.

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Abstract

The invention relates to the field of applied basic medical research, in particular to a method for constructing a neural tube deformity mouse model. According to the method, a pregnant mouse fed witha low-folic acid diet is interfered by physical or chemical teratogenic factors, low folic acid is used as a sensitizing factor, a human physiological environment is simulated, and the neural tube deformity of the mouse is induced, so that the success rate of constructing the model is greatly increased. The method provided by the invention is simple, good in repeatability, high in deformity rate,small in toxic and side effects on the pregnant mouse and low in embryo lethality.

Description

technical field [0001] The invention relates to the field of applied basic medical research, in particular to a method for constructing a neural tube defect mouse model. Background technique [0002] Neural tube defects (NTDs) is a high-incidence birth defect disease. It is the type with the highest morbidity and mortality among birth defects. It is also the most common and serious birth defect disease that affects the quality of the population in my country and the world. , NTDs malformation rate in newborns is as high as 0.1-0.5%. NTDs are deformities from the head to the spine due to neural tube failure or insufficiency, ranging from anencephaly to mild spina bifida: including anencephaly, deencephaly, microcephaly, macrocephaly, and hydrocephalus , meningocele, craniospina bifida, and spina bifida. NTDs usually occur in the early stages of pregnancy, and once irreversible, most of them die during pregnancy or within a short period of time after birth. The occurrence an...

Claims

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Application Information

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IPC IPC(8): A61K31/519A61K31/505A61P25/00
CPCA61K31/505A61K31/519A61P25/00
Inventor 裴培王珊张霆崔小岱
Owner 首都儿科研究所
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