Argopecten irradians concentricus and argopecten purpuratus interspecific hybridization seed preparation method

Abstract

The invention relates to the technical field of economic marine shellfish excellent offspring seed preparation, in particular to an Argopecten irradians concentricus and Argopecten purpuratus interspecific hybridization offspring seed preparation method capable of remarkably improving the hermaphrodite scallop interspecific hybridization offspring proportion. Selfing strains of continuously bred Argopecten irradians concentricus and Argopecten purpuratus are used as breeding scallops; the gonads of the two kinds of shellfish tend to be synchronously mature by manually controlling culture conditions; induced spawning of argopecten purpuratus seed shells is carried out to obtain high-concentration semen with the concentration larger than 106 pieces / ml; performing induced spawning induction on the argopecten irradians concentricus seed shells with mature gonad development to obtain a large amount of unfertilized or low-fertilization-rate ova, and performing interspecific hybridization on the ova and the obtained high-concentration semen of the argopecten purpuratus to prepare first-generation seeds of interspecific hybridization on a large scale. According to the method, a large number of unfertilized or low-fertilization-rate eggs of the Argopecten irradians concentricus are collected, and the Argopecten purpuratus high-concentration semen is used for insemination for interspecific hybridization, so that the interspecific hybridization efficiency of the Argopecten irradians concentricus and the Argopecten purpuratus can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of preparation of economical marine shellfish fine seedlings, in particular to a preparation method of interspecific hybrid seedlings of Gulf scallops and purple scallops, which can significantly increase the proportion of interspecific hybridization offspring of hermaphroditic scallops. Background technique [0002] The Gulf scallop (Argopecten irradians concentricus) is a hermaphrodite scallop native to the Atlantic coast of the United States. It was introduced to China in 1991 and successfully broke through artificial breeding. It has the characteristics of high rate and is quickly popularized in the southern coastal areas of my country. However, after more than 20 years of fully artificial cultivation, the germplasm of Gulf scallops has been seriously degraded, manifested as individual miniaturization, decreased stress resistance, decreased survival rate, low meat column rate and decreased yield, etc., wh...

AI Technical Summary

Problems solved by technology

[0004] However, hermaphroditic scallops can lay eggs and ovulate at the same time, and the eggs of the same species of scallops are more likely to be fertilized, that is, they are prone to self-fertilization or intraspecific fertilization, so the proportion of hybrid offspring will be less than 5% when using conventional spawning methods. %, which seriously affects the seed production efficiency of the first generation of Zimo hybrid scallops and the overall yield of cultured scallops. Therefore, it is very necessary to develop technologies to increase the proportion of interspecies hybrid progenies of Gulf of Mexico scallops and purple scallops