Method for obtaining cucumber regenerated plantlet by inducing gynogenesis by virtue of culture of unfertilized ovules
A technology for gynogenesis and regeneration of plants, applied in the field of plant tissue culture, can solve problems such as limiting haploid research and development, high deformity rate, and inability to apply breeding and research
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[0044] Materials and Methods
[0045] 1.1 Test material
[0046] The experimental materials used in this study are the F1 generation prepared from the European greenhouse type cucumber 65G as the female parent and the North China Mici type cucumber 228 as the male parent, provided by the cucumber research group of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences.
[0047] 1.2 Induction culture
[0048] Select female flowers 1-2 days before flowering, wash with running water and remove thorn tumors on the surface of the ovary, disinfect with 75% ethanol on the ultra-clean workbench for 30 seconds, then disinfect in 6.5% sodium hypochlorite solution for 15 minutes, rinse with sterile water repeatedly for 4 —5 times, blot the surface moisture with sterile filter paper, do not cut the ovary, and inoculate the complete ovary on the basis of Murashige and Skoog's medium (Murashige T. and Skoog F., 1962) plus TDZ pre-culture Preliminary in...
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