A method for inoculating and propagating Puccinia maize

A technology of Puccinia multicumulus and corn, which is applied in the direction of using spores, methods based on microorganisms, horticultural methods, etc., can solve the limitation of a large number of spores in the propagation speed of Puccinia multicumulus corn, and the propagation efficiency cannot meet the requirements Issues such as high demand for spores

Active Publication Date: 2021-07-06
CHINA AGRI UNIV
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Problems solved by technology

Since Puccinia maize is an obligate parasite, it can only survive and reproduce on live corn plants. Therefore, the expansion of Puccinia maize to obtain a large number of spores can only be carried out by co-cultivation with corn plants. , so that the multiplication rate of Puccinia maize and the source of a large number of spores are limited
[0003] Chinese patent 201510765142.1 discloses a single-spore multiplication method of Puccinia maize, the purpose of which is to obtain a pure strain of Puccinia maize, but the propagation efficiency of this method still cannot meet the requirements of artificial inoculation for spores high demand

Method used

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  • A method for inoculating and propagating Puccinia maize
  • A method for inoculating and propagating Puccinia maize
  • A method for inoculating and propagating Puccinia maize

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Embodiment 1

[0038] The inoculation propagation method (1) of embodiment 1 corn multi-heap Puccinia

[0039] The present embodiment provides a method for the inoculation and propagation of Puccinia maize multiheap, specifically as follows:

[0040] (1) Seedlings: select plump corn seeds, soak the seeds with 0.1% hydrogen peroxide at room temperature for 24h-48h, pour off the liquid or take out the seeds, cover the seeds with wet paper towels or wet cloth, and germinate in a ventilated place; The front end of the corn seed grows 1-2mm radicle before sowing. When sowing, the radicle faces upward and towards the center of the planting container. After cultivation, healthy corn seedlings at the three-leaf one-heart seedling stage are obtained;

[0041] (2) Spore elution on sick leaves and preparation of spore suspension: Spray 0.05% Tween-20 solution on sick leaves, about 2mL / sick leaves, cut sick leaves into 2-3cm 2 Place the small pieces in a large centrifuge tube, add 0.05% Tween-20 solut...

Embodiment 2

[0046] The inoculation propagation method (2) of embodiment 2 corn multi-heap Puccinia

[0047] This embodiment provides a method for inoculating and propagating Puccinia maize. The only difference between it and Embodiment 1 is that in step (5), steps (3) to (4) are repeated once.

[0048] Utilize the method of the present embodiment, after spraying the spore suspension, the spore heap on the blade is evenly distributed (such as figure 2 Shown); The harvested spores can obtain 3 pots of corn seedlings with good morbidity, and each pot harvests 30ng of spores, and obtains 100ng of spores altogether.

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Abstract

The invention relates to a method for multiplying plant pathogenic bacteria, in particular to a method for inoculating and multiplying Puccinia maize. The invention provides a method for inoculating and propagating Puccinia maize, comprising: spraying Tween-20 solution on diseased leaves, eluting spores on the diseased leaves into Tween-20 solution to prepare spore suspension , inoculated onto healthy corn seedlings, harvested and multiplied spores after cultivation, and used healthy corn seedlings for repeated transfer. The method has fast propagation speed and high efficiency of Puccinia multihelix, and can quickly obtain a large number of high-activity Puccinia multihelix spores, which greatly shortens the propagation period of Puccinia multihelix, and provides a great opportunity for the study of southern corn rust. Efficient strain propagation methods and technical support.

Description

technical field [0001] The invention relates to a method for multiplying plant pathogenic bacteria, in particular to a method for inoculating and multiplying Puccinia maize. Background technique [0002] Southern corn rust is an airborne disease caused by Puccinia polysora Underw., which occurs in many regions. In recent years, maize southern rust has become one of the main diseases affecting maize production. Therefore, people pay more and more attention to the research and control of Puccinia maize. The research on Puccinia multicumulus and southern corn rust is inseparable from artificial inoculation, and artificial inoculation usually requires a large number of highly active spores. Since Puccinia maize is an obligate parasite, it can only survive and reproduce on live corn plants. Therefore, the expansion of Puccinia maize to obtain a large number of spores can only be carried out by co-cultivation with corn plants. , so that the propagation speed of Puccinia maize an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14A01G7/06C12R1/645
CPCA01G7/06C12N1/14C12N3/00
Inventor 马占鸿黄莉群张克瑜李磊福孙秋玉王海光
Owner CHINA AGRI UNIV
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