51 results about "Rust fungus" patented technology

This invention relates to the field of genetic engineering technology, and provides a ZmPHYLL gene, the full-length nucleotide sequence of which is shown in SEQ ID NO: 1, and the coding region nucleotide sequence of which is shown in SEQ ID NO: 2. This invention also provides the application of the above-mentioned ZmPHYLL gene in inhibiting the germination of *Russula multifiliis* spores and improving the resistance of maize plants to southern maize rust. Simultaneously, this invention provides a method for improving the resistance of maize plants to southern maize rust. In this study, a ZmPHYLL gene was screened, and the expressed protein of this gene can effectively inhibit the germination of *Russula multifiliis* spores. Therefore, by regulating the overexpression of this gene, the resistance of maize to southern maize rust can be improved. This invention provides new gene resources for the creation of breeding materials and provides important scientific basis for the breeding of maize varieties highly resistant to southern maize rust.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The invention belongs to the technical field of plant genetic engineering and crop breeding, and particularly relates to application of wheat lectin receptor-like kinase TaSIT2 and a coding gene TaSIT2 thereof in improving stripe rust resistance of crops. A wheat lectin receptor-like kinase gene TaSIT2 is analyzed and screened by using a reverse genetics method, a TaSIT2 gene editing wheat plant is obtained by using an agrobacterium-mediated genetic transformation method through a gene editing technology, the resistance of the transgenic plant to wheat stripe rust physiological races is remarkably enhanced, and the wheat stripe rust physiological races can be obtained. It is shown that knockout of the TaSIT2 gene enhances the resistance of wheat to stripe rust, it is determined that the wheat lectin receptor-like kinase TaSIT2 gene plays a negative regulation role in wheat stripe rust resistance, and a new technical thought and gene resources are provided for wheat stripe rust resistance variety cultivation from the perspective of molecular biology. And more paths are provided for cultivating lasting broad-spectrum disease-resistant varieties.

The application relates to application of a wheat stripe rust genome SNP site combination in wheat stripe rust gene typing, wherein the wheat stripe rust genome SNP site combination comprises 9365 SNP sites, the physical positions of the 9365 SNP sites are determined based on wheat stripe rust reference genome Pst_104E_v13_all_ctg alignment; and the physical position information of the 9365 SNP sites is shown in Table 1. The application can efficiently, conveniently, accurately, economically and rapidly perform wheat stripe rust gene typing detection with high coverage.

The invention discloses a double-stranded RNA-nano material composite carrier and application thereof in prevention and treatment of soybean rust. A fungus extracellular membrane protein PpAFF gene of the soybean rust pathogen is taken as a target, a transcript of the fungus extracellular membrane protein PpAFF gene is taken as a template to synthesize double-stranded RNA, and the double-stranded RNA is sprayed on the surfaces of soybean leaves, so that the PpAFF gene of the soybean rust pathogen can be silenced, and the symptom of the soybean rust disease can be effectively relieved. The base sequence of the gene is as shown in SEQ ID NO: 1. The synthesized nano material MNB can inhibit germination of soybean rust uredospores, stimulate immunoreactions of various plants such as soybeans, wheat, rice and hot peppers, and improve the resistance of crops to pathogenic bacteria. The prevention and control method of spraying the MNB-dsPpAFF compound on the plant surface is green and safe, the prevention and control effect is remarkable, and a very potential solution is provided for prevention and control of important crop fungal diseases such as soybean rust and rice blast.

The invention belongs to the technical field of genetic engineering breeding, and discloses a wheat disease-resistant factor gene TaPsaF and application thereof. A TaPsaF gene sequence is analyzed, and a TaPsaF-GFP fusion protein is constructed, so that the localization of the TaPsaF-GFP fusion protein in cell chloroplast is determined. An overexpression and silence vector of the TaPsaF gene is successfully constructed by using a gene engineering technology, and wheat is transformed by using an agrobacterium-mediated method to obtain a transgenic wheat material. Functional verification shows that over-expression of the TaPsaF gene can significantly enhance the resistance of wheat to stripe rust, and silencing of the gene leads to reduction of the resistance. Gene resources and a technical path are provided for wheat stripe rust resistance molecular breeding, the blank of the TaPsaF protein in biological stress response mechanism research is filled up, and the TaPsaF protein has important significance for guaranteeing food safety.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat VOZ transcription factor or a coding gene thereof in regulation and control of stripe rust resistance of crops. The invention systematically reveals that wheat transcription factors TaVOZ1 and TaVOZ2 play a positive regulation role in immune response of wheat to stripe rust for the first time, and enriches a functional spectrum of a VOZ family in plant immune regulation; meanwhile, the broad-spectrum resistance of an overexpression material to wheat stripe rust (Pst) is remarkably enhanced by overexpressing the encoding gene of the wheat VOZ transcription factor in wheat, and the wheat VOZ transcription factor has practical breeding application potential, so that the wheat VOZ transcription factor encoding gene provided by the invention can be used as an important candidate for breeding disease-resistant molecules; a new way and a new strategy are provided for green prevention and control of wheat diseases.

The invention discloses a stripe rust resistance gene YrRC1 derived from roegneria ciliata as well as an encoding protein and application thereof. The stripe rust resistance gene YrRc1 provided by the invention is derived from a roegneria ciliata 2Sc chromosome, the nucleotide sequence of the gene is SEQ ID NO.1, and the amino acid sequence of the gene is SEQ ID NO.2. The invention further discloses a preparation method of the stripe rust resistance gene YrRc1. The invention also protects the application of the YrRC1 in improving the disease resistance of plants. The YrRc1 gene is induced by puccinia striiformis to realize rapid up-regulation expression; a VIGS system is utilized to silence YrRc1 in a disease-resistant material DA2Sc to enhance the plant susceptibility; the YrRc1 gene is introduced into a stripe rust susceptible variety Fielder through a transgenic technology, so that the stripe rust resistance can be remarkably improved. Therefore, the YrRc1 can positively regulate and control the stripe rust resistance and has important significance on wheat disease-resistant breeding.

This invention discloses TaALDH7 Application of genes in regulating crop resistance to stripe rust and breeding disease-resistant varieties. This invention screened key genes regulating wheat resistance to stripe rust through combined transcriptomic and metabolomic analysis. TaALDH7 Through transient overexpression and gene silencing technology, TaALDH7 The function of the gene in wheat resistance to stripe rust was analyzed; the experimental results showed that, compared with the control group, the gene was overexpressed in wheat. TaALDH7 The disease index and fungal biomass of the gene were significantly reduced, and the colony area and hyphal length of stripe rust fungus were significantly decreased; conversely, in silent wheat... TaALDH7 The gene significantly reduces the resistance response of wheat to stripe rust, indicating that... TaALDH7 Genes act as positive regulators in the wheat's resistance to stripe rust. This invention shows promise for applications in breeding stripe rust-resistant wheat varieties and improving wheat's resistance to stripe rust fungi.

The present application relates to the application of wheat AvrLr15 gene, primer set and kit in detecting and / or identifying the virulence of wheat leaf rust fungus, and belongs to the technical field of pathogenic microorganism detection.The present application provides the application of wheat AvrLr15 gene in detecting and / or identifying the virulence of wheat leaf rust fungus, wherein the CDS region nucleotide sequence of the AvrLr15 gene is shown as SEQ ID NO.1.The primer set of the present application can directly detect and / or identify the virulence of wheat leaf rust fungus to the wheat leaf rust resistant material TcLr15, and has good specificity, reliability and stability, and can be used as a primer set of specific molecular marker for detecting and / or identifying the virulence of wheat leaf rust fungus to TcLr15.

The invention provides application of a TaTN3 gene in regulation and control of wheat stripe rust resistance, and belongs to the technical field of gene engineering. An amino acid sequence coded by the TaTN3 gene is shown as SEQ ID No.2, and the TaTN3 gene is positioned on a cell membrane. The invention also constructs a gene silencing strain and an overexpression strain, and results show that the TaTN3 gene is silenced in a wheat material, and the resistance of wheat to stripe rust is reduced; through overexpression of the TaTN3 gene, the resistance of wheat to puccinia striiformis is improved. It is proved that the TaTN3 protein and the TaTN3 gene have the effect of regulating and controlling the wheat stripe rust resistance. Gene resources and technical thoughts are provided for cultivation of stripe rust resistant wheat varieties, and the application value is high.

This invention belongs to the field of bioengineering technology and relates to a wheat receptor protein kinase TaFER-NW and its applications. The amino acid sequence of the wheat receptor protein kinase TaFER-NW provided by this invention is shown in SEQ ID NO:1, and the encoding ORF sequence of the wheat receptor protein kinase TaFER-NW is shown in SEQ ID NO:2. This invention involves gene editing in Fielder wild-type materials to obtain gene-edited mutant plants of TaFER-NW that exhibit resistance to the stripe rust fungus CYR31. This invention provides a new technical approach for breeding stripe rust-resistant varieties, confirming that this gene plays a negative regulatory role in wheat stripe rust resistance, and providing new gene resources for wheat stripe rust resistance breeding.

The present application belongs to the field of agricultural microorganism technology, and particularly relates to a Paenibacillus polymyxa, a bacterial agent thereof and application. Paenibacillus polymyxa GC-13, the strain preservation number of which is CGMCC No.32824, and the 16S rDNA sequence of which is shown as SEQ ID NO:1. It is verified that the Paenibacillus polymyxa Paenibacillus polymyxa GC-13 has strong antagonistic effect on the uredospores of Puccinia striiformis, and on the pathogenic bacteria of wheat take-all disease and wheat scab. It is found through pot experiment that the Paenibacillus polymyxa Paenibacillus polymyxa GC-13 has remarkable prevention and treatment effect on the plant fungal diseases of wheat stripe rust, wheat take-all disease and wheat scab. The present application provides a new microbial resource for prevention and treatment of plant fungal diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of wheat receptor-like cytoplasm kinase TaRLCK118 or a coding gene thereof in the aspect of improving stripe rust resistance of crops. A reverse genetics method verifies that the TaRLCK118 gene plays a positive regulation role in wheat stripe rust resistance, so that the expression quantity of wheat receptor cytoplasm kinase TaRLCK118 can be improved by improving the expression of the TaRLCK118 gene in a receptor crop, and the resistance of the crop to stripe rust is improved; and gene resources and biological materials are provided for cultivation of stripe rust resistant wheat varieties.

The invention belongs to the technical field of gene engineering, and relates to application of a transcription factor TaPHR2 in wheat stripe rust resistance. According to the invention, a reverse genetics method is adopted to verify the function of the TaPHR2 gene in the interaction of wheat and stripe rust, the TaPHR2 gene is induced to express by a non-affinity race CYR23 of wheat stripe rust, and a virus-mediated gene silencing technology is adopted to instantly silence the TaPHR2 gene to verify that the TaPHR2 gene plays a positive regulation role in the defense reaction of wheat stripe rust resistance. An overexpression vector is constructed in the full length of the TaPHR2 gene, a transgenic plant for overexpressing the TaPHR2 gene is obtained through agrobacterium-mediated genetic transformation, the resistance of the transgenic plant to wheat stripe rust physiological race CYR32 is remarkably enhanced, and more thoughts are provided for cultivating disease-resistant varieties.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of wheat VOZ transcription factors or their encoding genes in regulating crop stripe rust resistance. This invention systematically reveals for the first time the positive regulatory role of wheat transcription factors TaVOZ1 and TaVOZ2 in the immune response of wheat to stripe rust, enriching the functional spectrum of the VOZ family in plant immune regulation; simultaneously, this invention significantly enhances the resistance of overexpressed materials to wheat stripe rust fungus by overexpressing the encoding gene of wheat VOZ transcription factors. Pst The broad-spectrum resistance of wheat VOZ transcription factor has practical breeding application potential, thus proving that the wheat VOZ transcription factor encoding gene provided by this invention can be an important candidate for disease-resistant molecular breeding, providing a new approach and strategy for green prevention and control of wheat diseases.

This invention discloses the disease resistance function and application of the CC domain of CNL protein 2541 involved in wheat stripe rust resistance, belonging to the field of functional gene technology. This invention provides the application of the 2541-CC gene domain in regulating wheat stripe rust resistance, and provides a recombinant expression vector overexpressing the 2541-CC gene domain, as well as a method for constructing plant germplasm overexpressing the 2541-CC gene domain using the recombinant expression vector. This invention found that plants overexpressing the 2541-CC gene domain exhibited significant resistance, increased leaf hypersensitive necrosis, reduced stripe rust urediniospores, increased reactive oxygen species, increased cell necrosis, and inhibited stripe rust growth and development, thus confirming that the 2541-CC gene domain is a key domain in the 2541 gene's disease resistance signal transduction.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor-like kinase TaSPR12 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSPR12 is analyzed and screened by using a reverse genetics method, and the gene is induced to express by stripe rust germs. According to the present invention, the CRISPR-Cas9 gene editing technology is adopted to knock out the wheat receptor kinase gene TaSPR12 to create the function deletion mutant, and the resistance of the wheat stripe rust main epidemic race is improved; the invention determines that the wheat receptor kinase gene TaSPR12 plays a negative regulation role in wheat stripe rust resistance, and can be used for genetic improvement of wheat rust resistance.

The invention provides a wheat-derived stripe rust effect protein Pst8724, a coding gene and application, and belongs to the technical field of gene engineering. The invention provides a stripe rust effect protein Pst8724 from wheat, the amino acid sequence of the stripe rust effect protein Pst8724 is as shown in SEQ ID No.1, and the nucleotide sequence of the coding gene of the stripe rust effect protein Pst8724 is as shown in SEQ ID No.2. According to the invention, RNA interference (RNAi) and overexpression wheat plants of Pst8724 are created through a genetic transformation technology. Disease resistance identification results show that Pst8724 positively regulates pathogenicity of puccinia striiformis to wheat, histological and cytological observation shows that accumulation of H2O2 and expression of PR genes can be promoted by reduction of expression quantity of Pst8724 in interaction of puccinia striiformis and wheat, and resistance of wheat to puccinia striiformis is enhanced. The invention provides a new material for the cultivation of the stripe rust resistant wheat variety.

The present application belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease resistance factor TaPKS26 in leaf rust prevention and treatment. The CDS sequence of the coding gene of the wheat disease resistance factor TaPKS26 is shown as SEQ ID NO:1, and the present application adopts an agrobacterium-mediated method to obtain a transgenic wheat strain overexpressing the gene TaPKS26. After being infected with the physiological race THT and PHT of leaf rust, the transgenic wheat strain overexpressing the gene TaPKS26 is compared with Fielder wheat, and the results show that the resistance of the transgenic wheat strain to wheat leaf rust is enhanced, and the expression amount of a disease resistance related marker gene is up-regulated, indicating that the wheat disease resistance factor TaPKS26 plays a positive regulation role in the defense response of wheat against leaf rust. The present application provides a new gene resource and technical idea for breeding of a leaf rust disease resistance variety.

This invention belongs to the field of genetic engineering technology and discloses the application of the transcription factor TaNAC67 in regulating wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown in SEQ ID NO: 1. This transcription factor can induce cell necrosis and plays a positive regulatory role in the interaction between wheat and stripe rust fungus. The gene encoding this transcription factor... TaNAC67 This is a disease resistance-related gene that is strongly induced by stripe rust infection in the incompatible interaction between plants and stripe rust fungi. Its nucleotide sequence is shown in SEQ ID NO: 2. Based on these characteristics, this invention further obtains an overexpressing gene. TaNAC67 The transgenic plants were tested and found to exhibit resistance to stripe rust. This invention provides genetic resources and technical support for the subsequent genetic improvement of wheat materials resistant to stripe rust.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of an encoding gene thereof in regulating plant resistance to stripe rust. The application analyzes the function of the wheat magnesium transporter TaNIPA8-6B through gene editing and overexpression, finds that the wheat magnesium transporter TaNIPA8-6B is induced to express in the reaction of wheat and stripe rust in compatible and incompatible systems, and creates gene editing and overexpression TaNIPA8-6B mutant plants of the gene, and identifies that the gene knockout mutant of the gene shows a disease susceptibility to the incompatible race CYR23 of stripe rust, and the overexpression mutant plant shows a disease resistance to the compatible race CYR32 of stripe rust, and determines that TaNIPA8-6B the gene plays a positive regulation role in the disease resistance of wheat to stripe rust, and provides a high-quality germplasm resource for the resistance of wheat to stripe rust.

This invention belongs to the fields of plant genetic engineering and crop breeding technology, specifically relating to wheat lectin receptor kinase TaSIT2 and its encoding gene. TaSIT2 Applications in improving crop resistance to stripe rust. This invention utilizes reverse genetics to analyze and screen wheat lectin receptor kinase genes. TaSIT2 Through gene editing technology and Agrobacterium-mediated genetic transformation, we can obtain... TaSIT2 Gene-edited wheat plants showed significantly enhanced resistance to wheat stripe rust races, indicating that knockout... TaSIT2 Genes enhanced wheat resistance to stripe rust and identified wheat lectin receptor kinases. TaSIT2 Genes play a negative regulatory role in wheat resistance to stripe rust, providing new technical ideas and gene resources for breeding wheat varieties resistant to stripe rust from a molecular biology perspective, and offering more pathways for breeding varieties with long-lasting and broad-spectrum disease resistance.

This invention discloses a double-stranded RNA-nanomaterial composite carrier and its application in the control of soybean rust. Using the PpAFF gene, a fungal extracellular membrane protein of soybean rust fungus, as a target, and its transcript as a template, double-stranded RNA is synthesized and sprayed onto the surface of soybean leaves. This silences the PpAFF gene, effectively alleviating soybean rust symptoms. The base sequence of this gene is shown in SEQ ID NO:1. The synthesized nanomaterial MNB can inhibit the germination of urediniospores of soybean rust fungus and stimulate immune responses in various plants such as soybean, wheat, rice, and pepper, improving crop resistance to the pathogen. The method of spraying the MNB-dsPpAFF complex onto the plant surface is green, safe, and highly effective. This invention provides a highly promising solution for the control of fungal diseases in important crops such as soybean rust and rice blast.