105 results about "Rust fungus" patented technology

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention belongs to the field of biological agriculture, and particularly relates to a wheat negative regulatory factor TaEDR2 and application thereof. The amino acid sequence of the wheat negative regulatory factor encoding protein provided by the invention is as shown in SEQ ID NO: 1, and the nucleotide sequence of the wheat negative regulatory factor TaEDR2 is as shown in SEQ ID NO: 2. According to the invention, gene editing is carried out in a Fielder wild type material, and a TaEDR2 gene editing plant which shows resistance to stripe rust CYR32 is obtained. It is determined that the TaEDR2 gene plays a negative regulation role in wheat stripe rust resistance, a stripe rust resistant wheat material is created by using the negative regulation factor, and new gene and germplasm resources are provided for wheat stripe rust resistant breeding.

The invention provides a wheat stripe rust early warning method, system and device, a storage medium and a program product, and relates to the technical field of agriculture, and the method comprises the steps: obtaining the spore capture amount, meteorological data and field morbidity of a to-be-monitored farmland; the to-be-monitored farmland is used for planting to-be-monitored wheat, the spore capture amount is the capture number of puccinia striiformis wheat specialized spores, and the field morbidity is the probability that the to-be-monitored wheat is infected with wheat stripe rust; based on the meteorological data, the spore capture amount and the field morbidity, constructing a wheat stripe rust prediction model; and based on the wheat stripe rust prediction model, performing wheat stripe rust monitoring and early warning on the to-be-monitored farmland. By means of the mode, efficient, accurate and real-time monitoring and early warning can be conducted on the wheat stripe rust without depending on manual investigation, the accuracy rate is high, the efficiency is high, and outbreak of the wheat stripe rust can be effectively prevented.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention provides wheat receptor cytoplasm kinase TaRIPK8 and application thereof in improvement of stripe rust resistant varieties of cereal crops, and belongs to the technical field of biological agriculture. The invention provides application of wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of stripe rust resistance of cereal crops and / or improvement or cultivation of stripe rust resistance varieties of the cereal crops. The wheat receptor cytoplasm kinase TaRIPK8 comprises protein with an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The invention further provides application of the wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of the stripe rust resistance character of the cereal crops and / or improvement or cultivation of the stripe rust resistance varieties of the cereal crops. The receptor-like cytoplasm kinase TaRIPK8 participates in negative regulation of wheat on stripe rust, so that a high stripe rust resistance strain can be created on the basis, and an excellent wheat material is provided for cultivation of stripe rust resistance varieties.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The invention discloses a wheat stripe rust effect protein gene Pst4593 and application thereof, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the wheat stripe rust effect protein gene Pst4593 is shown as SEQ ID NO: 1, the gene positively regulates pathogenicity of stripe rust to wheat, silence of the wheat stripe rust effect protein gene Pst4593 can improve resistance of wheat to stripe rust, and relative expression quantities of wheat related defense genes TaPR1, TaPR2 and TaPR5 are remarkably improved. The wheat stripe rust effect protein gene Pst4593 is related to induced expression of stripe rust aspirator, and the protein coded by the wheat stripe rust effect protein gene Pst4593 also has lipase activity and can influence pathogenicity of stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention belongs to the technical field of bioengineering, and relates to a wheat secretion carrier related membrane protein TaSCAMP6 and application thereof. The amino acid sequence of the wheat secretion vector related membrane protein TaSCAMP6 provided by the invention is as shown in SEQ ID NO: 2; the nucleotide sequence for coding the wheat secretion vector related membrane protein TaSCAMP6 gene is as shown in SEQ ID NO: 1; according to the invention, the function of the TaSCAMP6 gene is researched through a genetic means, transgenosis is carried out in a Fielder wild type material, a TaSCAMP6 overexpression plant is obtained, and verification shows that the resistance of TaSCAMP6 overexpression to stripe rust is enhanced, the gene is determined to play a positive regulation role in stripe rust resistance of wheat, and a new gene resource is provided for stripe rust resistance breeding of wheat.

This invention relates to the field of genetic engineering technology, and provides a ZmPHYLL gene, the full-length nucleotide sequence of which is shown in SEQ ID NO: 1, and the coding region nucleotide sequence of which is shown in SEQ ID NO: 2. This invention also provides the application of the above-mentioned ZmPHYLL gene in inhibiting the germination of *Russula multifiliis* spores and improving the resistance of maize plants to southern maize rust. Simultaneously, this invention provides a method for improving the resistance of maize plants to southern maize rust. In this study, a ZmPHYLL gene was screened, and the expressed protein of this gene can effectively inhibit the germination of *Russula multifiliis* spores. Therefore, by regulating the overexpression of this gene, the resistance of maize to southern maize rust can be improved. This invention provides new gene resources for the creation of breeding materials and provides important scientific basis for the breeding of maize varieties highly resistant to southern maize rust.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention belongs to but not limited to the technical field of plant protection, and particularly relates to application and method for improving wheat stripe rust resistance through allogenic material treatment, 250 micromoles of luteolin is used for culturing stripe rust fungus spores, germination of the stripe rust fungus spores is remarkably inhibited, 500 micromoles of luteolin is used for pretreating wheat leaves, spraying is carried out once a day, spraying is carried out three times in total, and the yield of the wheat stripe rust resistance is increased. After the leaves absorb the luteolin, the puccinia striiformis is inoculated, and the result shows that the endogenous luteolin content of the wheat plants treated by the luteolin is increased, the infection area of the puccinia striiformis is remarkably reduced, the number of puccinia striiformis spores on the leaves is remarkably reduced, and the biomass of the puccinia striiformis is remarkably reduced. The invention proves that the occurrence of wheat stripe rust can be inhibited by exogenous luteolin treatment, and a certain reference is provided for stripe rust disease-resistant mechanism research and prevention and control measures.

The invention discloses application of a wheat gene TaALD1 in improving wheat stripe rust resistance, relates to the technical field of biology, and has the technical key points that the nucleotide sequence of the TaALD1 is shown as SEQ ID No.1. According to the invention, TaALD1 is subjected to up-regulation expression after being infected by stripe rust, and virus-induced gene silencing is adopted to determine that TaALD1 plays a positive regulation role in wheat stripe rust resistance; and meanwhile, the transgenic ALD1 plant can obviously improve the stripe rust resistance of the wheat, has a better application prospect in the cultivation of disease-resistant wheat varieties, and lays an important foundation for the disease-resistant breeding of the wheat.

The invention discloses a terahertz time-domain spectroscopy-based wheat stripe rust uredospore optical parameter extraction method. The method comprises the following steps: mixing wheat stripe rust uredospore powder and polyethylene powder, and tabletting; a transmission module of the terahertz time-domain spectrometer is used for collecting time-domain spectral data of the mixed tablets in a nitrogen environment; carrying out filtering processing on the collected time-domain spectral data to eliminate high-frequency noise; main waves of the time domain signals are reserved, the echo position is estimated, sampling points are randomly generated through normal distribution to dynamically correct the echo signals, and time domain interference is reduced; and performing fast Fourier transform on the corrected time domain signal, and calculating the refractive index, the absorption coefficient and the dielectric constant of the wheat stripe rust uredospores through frequency domain amplitude and phase information on the basis of an improved Dorne-Duvillaret model. Through sample preparation optimization, echo correction and model improvement, the measurement precision can be remarkably improved.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor kinase TaSRK1 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSRK1 is analyzed and screened by using a reverse genetics method, it is proved that TaSRK1 is induced to express by stripe rust germs, a CRISPR-Cas9 gene editing technology is adopted to knock out TaSRK1 to create a function deletion mutant, the resistance to main epidemic microspecies of wheat stripe rust is improved, and the resistance to the main epidemic microspecies of wheat stripe rust is improved. It is determined that the wheat receptor kinase gene TaSRK1 plays a negative regulation role in wheat stripe rust resistance, and the wheat stripe rust resistance gene TaSRK1 can be used for wheat stripe rust resistance genetic improvement.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The utility model discloses wheat stripe rust collecting and storing equipment which comprises a handle, a collector is fixedly connected to the top of the handle, a collecting tank is detachably connected to the bottom of the collector, a material suction opening is fixedly connected to one end of the collector, and a collecting assembly is arranged on the outer wall of the material suction opening. According to the wheat stripe rust fungus collecting and storing equipment, materials can be sucked in through a material suction opening, in the suction process, wheat leaves can be held by the other hand to be straightened, a brush is attached to the wheat leaves, rust fungi on the surfaces of the wheat leaves can be treated through back-and-forth pulling, in addition, when the brush conducts brushing, the wheat leaves can be conveniently cleaned, and the wheat leaves can be conveniently stored. Under the supporting of the supporting spring, the connecting rod can move back and forth through the connecting plate, through the arrangement of the supporting spring, the flexibility, adaptability and durability of the brush can be enhanced, the spring can ensure that the brush and the surface keep proper contact force for uneven wheat leaves, and therefore the material suction effect is improved.

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The present invention belongs to the field of bio-agriculture, and particularly relates to a wheat negative regulator TaEDR2 and its application. The amino acid sequence of the protein encoded by the wheat negative regulator provided by the present invention is shown in SEQ ID NO: 1, and the nucleotide sequence of the wheat negative regulator TaEDR2 is shown in SEQ ID NO: 2. The present invention performs gene editing in the Fielder wild-type material to obtain TaEDR2 gene-edited plants that show resistance to the stripe rust fungus CYR32. The present invention determines that the TaEDR2 gene plays a negative regulatory role in wheat resistance to stripe rust, and uses this negative regulator to create wheat materials resistant to stripe rust, providing new genes and germplasm resources for wheat breeding for resistance to stripe rust.

The invention belongs to the technical field of plant genetic engineering and crop breeding, and particularly relates to application of wheat lectin receptor-like kinase TaSIT2 and a coding gene TaSIT2 thereof in improving stripe rust resistance of crops. A wheat lectin receptor-like kinase gene TaSIT2 is analyzed and screened by using a reverse genetics method, a TaSIT2 gene editing wheat plant is obtained by using an agrobacterium-mediated genetic transformation method through a gene editing technology, the resistance of the transgenic plant to wheat stripe rust physiological races is remarkably enhanced, and the wheat stripe rust physiological races can be obtained. It is shown that knockout of the TaSIT2 gene enhances the resistance of wheat to stripe rust, it is determined that the wheat lectin receptor-like kinase TaSIT2 gene plays a negative regulation role in wheat stripe rust resistance, and a new technical thought and gene resources are provided for wheat stripe rust resistance variety cultivation from the perspective of molecular biology. And more paths are provided for cultivating lasting broad-spectrum disease-resistant varieties.

The application relates to application of a wheat stripe rust genome SNP site combination in wheat stripe rust gene typing, wherein the wheat stripe rust genome SNP site combination comprises 9365 SNP sites, the physical positions of the 9365 SNP sites are determined based on wheat stripe rust reference genome Pst_104E_v13_all_ctg alignment; and the physical position information of the 9365 SNP sites is shown in Table 1. The application can efficiently, conveniently, accurately, economically and rapidly perform wheat stripe rust gene typing detection with high coverage.

The invention discloses a fungicide composition for preventing and controlling sunflower white rust and its application. The active ingredients of the fungicide composition are composed of Bacillus amyloliquefaciens and Rhodomyces thulophilus. The two active ingredients in the fungicide composition have a significant synergistic effect, and have an excellent control effect on sunflower white rust caused by infection of sharp herb white rust fungus. In addition, the amount of the fungicide composition used is small, which can reduce the amount of chemical pesticides used and reduce pollution to the environment.

The invention discloses a double-stranded RNA-nano material composite carrier and application thereof in prevention and treatment of soybean rust. A fungus extracellular membrane protein PpAFF gene of the soybean rust pathogen is taken as a target, a transcript of the fungus extracellular membrane protein PpAFF gene is taken as a template to synthesize double-stranded RNA, and the double-stranded RNA is sprayed on the surfaces of soybean leaves, so that the PpAFF gene of the soybean rust pathogen can be silenced, and the symptom of the soybean rust disease can be effectively relieved. The base sequence of the gene is as shown in SEQ ID NO: 1. The synthesized nano material MNB can inhibit germination of soybean rust uredospores, stimulate immunoreactions of various plants such as soybeans, wheat, rice and hot peppers, and improve the resistance of crops to pathogenic bacteria. The prevention and control method of spraying the MNB-dsPpAFF compound on the plant surface is green and safe, the prevention and control effect is remarkable, and a very potential solution is provided for prevention and control of important crop fungal diseases such as soybean rust and rice blast.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to paenibacillus polymyxa as well as a fungicide and application thereof. The invention discloses paenibacillus polymyxa GC-13, the preservation number of the paenibacillus polymyxa GC-13 is CGMCC (China General Microbiological Culture Collection Center) No.32824, and the 16S rDNA (Ribosomal Deoxyribose Nucleic Acid) sequence of the paenibacillus polymyxa GC-13 is as shown in SEQ ID NO: 1. Through verification, the paenibacillus polymyxa GC-13 has a relatively strong antagonistic effect on uredospores of wheat stripe rust bacteria, and pathogenic bacteria of wheat scab and wheat basal stem rot, and the paenibacillus polymyxa GC-13 has a relatively strong antagonistic effect on the uredospores of the wheat stripe rust bacteria and the pathogenic bacteria of the wheat scab and the wheat basal stem rot. A pot experiment shows that the paenibacillus polymyxa GC-13 has a remarkable prevention and treatment effect on plant fungal diseases such as wheat stripe rust, wheat stem rot and wheat scab. The paenibacillus polymyxa GC-13 has the advantages that the paenibacillus polymyxa GC-13 can be used for preventing and treating the plant fungal diseases such as the wheat stripe rust, the wheat stem rot and the wheat scab; the invention provides a new microbial resource for preventing and treating fungal diseases of plants.

The present application provides a method, storage medium and device for predicting the disease index of southern corn rust, and the method comprises: predicting the seasonal epidemic progress of multiple piles of rust spores that cause southern rust, obtaining a growth index equation of multiple piles of rust spores, and the growth index equation of multiple piles of rust spores is used to illustrate the influence of temperature and rainfall on the growth index of multiple piles of rust spores; obtaining a corn southern rust fitness index formula according to the growth index equation of multiple piles of rust spores, and the corn southern rust fitness index formula is used to illustrate the adaptability of multiple piles of rust spores to survive in a certain area and the fitness of corn southern rust in the area when the temperature and rainfall are different. Index; from the beginning of the corn inoculation season, assuming that the number of multiple piles of handle rust spores in the air remains unchanged, diffusion events will occur when the wind speed exceeds 1 meter / second. As the cumulative diffusion events increase, the number of multiple piles of handle rust spores in the air changes with the wind speed and follows the negative exponential decrease condition, fitting the data set of wind speed and multiple piles of handle rust spore growth index in the corn inoculation season in historical years, obtaining the diffusion index of the multiple piles of handle rust spore growth index, and expressing the diffusion index with an exponentially decreasing function equation; according to the condition that rainfall events will cause the deposition of multiple piles of handle rust spores, fitting the multiple piles of handle rust spore concentrations and daily average temperatures in historical years to obtain a disease index prediction model. Through the scheme of this application, the potential distribution range of southern corn rust can be predicted, the possible time and outbreak period of southern corn rust can be obtained, and technical support can be provided for precise application of pesticides.