Method for establishing griffinia liboniana bulblet regeneration system by taking stamen as explant

A technology of small bulbs and explants, which is applied in the field of establishment of blue crystal flower small bulb regeneration system, to achieve the effects of improving induction rate and proliferation efficiency, shortening propagation cycle, and high callus induction rate

Inactive Publication Date: 2017-01-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is no report on the establishment of a blue crystal flower propagation system

Method used

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  • Method for establishing griffinia liboniana bulblet regeneration system by taking stamen as explant
  • Method for establishing griffinia liboniana bulblet regeneration system by taking stamen as explant
  • Method for establishing griffinia liboniana bulblet regeneration system by taking stamen as explant

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Extraction and processing of explants

[0040] During the flowering period of the blue crystal flower in June, young flower buds (buds not opened) with a length of 1.5-2.5 cm were picked, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0041] Transfer the refrigerated flower bud material to a beaker, soak in water with a little Tween and detergent added dropwise for 15 minutes, then cover the mouth of the beaker with gauze, and rinse the gauze with running water for 1 hour. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 8min. Reagents are fully exposed.

[0042] Finally, rinse 3 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0043] (2) Explant inoculation and callus induction

...

Embodiment 2

[0056] (1) Extraction and processing of explants

[0057] In the flowering period of the blue crystal flower in June, young flower buds with a length of 1.5-2.5 cm (buds are not opened) are collected, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0058] The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 15 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.

[0059] Finally, rinse 4 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0060] (2) Explant inoculati...

Embodiment 3

[0073] (1) Extraction and processing of explants

[0074] In the flowering period of the blue crystal flower in June, young flower buds with a length of 1.5-2.5 cm (the buds are not opened) are collected, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0075]The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 20 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.

[0076] Finally, rinse with sterile water 5 times, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0077] (2) Explant inocul...

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Abstract

The invention discloses a method for establishing a griffinia liboniana bulblet regeneration system by taking stamen as an explant. The method comprises the steps of taking a flower bud of griffinia liboniana, carrying out disinfection treatment, then cutting out the stamen, inoculating the stamen onto a callus induction medium, and culturing to obtain bulblet-containing callus; stripping a bulblet from the callus, transferring the stripped bulblet into a multiplication medium, and culturing to obtain a bulblet proliferation body; inoculating the bulblet proliferation body into a rooting medium, and carrying out enlargement and root induction on the bulblet to obtain a griffinia bulblet. According to the method, the griffinia bulblet regeneration system is obtained by taking the stamen as the explant, and carrying out disinfection treatment, callus induction, bulblet regeneration and proliferation, bulblet enlargement and rooting culture; after the method is adopted, the contamination rate is remarkably reduced, the induction rate and the multiplication efficiency are improved, and the propagation cycle is shortened.

Description

technical field [0001] The invention relates to the technical field of rapid plant propagation, in particular to a method for establishing a blue crystal bulblet regeneration system with stamens as explants. Background technique [0002] Griffinia (Griffinia) belongs to Amaryllidaceae (Amarylllidaceae), a tropical bulbous flower native to Brazil; it mostly grows in dense vegetation and humid tropical rainforest, with a diameter of 2-3cm, and is a miniature bulbous plant. Under natural growth conditions, aquamarine flowers generally form new plants by dividing the bulbs into lateral bulblets. Plants of this genus are not self-fertile, and reproduction by seed requires two different individuals, and it takes up to 8 months for the seeds to mature. Therefore, the long vegetative growth period and low natural reproduction coefficient have become two important factors restricting the proliferation of plants of the genus Azurite. [0003] In addition, due to the shrinking of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 夏宜平任梓铭李岳张栋吴昀孙敏译
Owner ZHEJIANG UNIV
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