2 results about "Aphis gossypii" patented technology

A method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii is provided, relating to the technical field of cotton safety evaluation. The method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii includes planting the gene-edited low-gossypol cotton indoors, collecting young leaves and inoculating nymphal Aphis gossypii on the leaves, rearing the nymphal Aphis gossypii, recording a number of adult Aphis gossypii deaths and a number of newly hatched nymphal Aphis gossypii, calculating an Aphis gossypii mortality rate and an average Aphis gossypii productivity, and evaluating the influence.

This invention belongs to the field of fruit tree resistance gene application technology, and relates to an apple transcription factor MYB79 gene and its application in improving apple resistance to the woolly aphid. The amino acid sequence of the protein encoded by the MYB79 gene is shown in SEQ ID NO:1, and the nucleotide sequence is shown in SEQ ID NO:2. This invention constructs a plant expression vector carrying the MYB79 gene and recombinant Agrobacterium, and obtains MYB79-overexpressing apple plants through Agrobacterium-mediated transformation. Functional verification results show that MYB79 gene overexpression can significantly improve apple resistance to the woolly aphid, reduce the feeding efficiency and survival rate of the woolly aphid, and significantly shorten the feeding time of the woolly aphid in the phloem of the transgenic plants, resulting in a significant decrease in survival rate, while having no significant impact on the normal growth and development of the apple plants. This invention provides new gene resources and technical support for molecular breeding of apples resistant to the woolly aphid and innovation of insect-resistant germplasm.