69 results about "Aphis gossypii" patented technology

Nucleic acid sequence encoding Allium fistulosum leaf agglutinin (AFAL) is disclosed. The invention provides Allium fistulosum leaf agglutinin (AFAL) recombinant protein, its encoding nucleotides, primers and the process of preparation thereof, said recombinant protein is useful for insect control and haemagglutination activity. AFAL is found more toxic to sap sucking insect pest Aphis gossypii (cotton aphid) and Bemisia tabaci (whiteflies) as compared to known Allium sativum leaf agglutinin. AFAL can be used in the development of transgenic plants for resistance against sap sucking and chewing pests.

The invention discloses a nicotine-containing botanical synergist compound insecticide which is prepared by using nicotine and turpentine as main components and adding an assistant through compounding. The forms of the nicotine-containing botanical synergist compound insecticide comprise environment-friendly missible oil, microemulsion or emulsion in water; and the nicotine-containing botanical synergist compound insecticide comprises the following components: 1.0-50.0% of nicotine, 5.0-40.0% of turpentine and the balance of the assistant. The nicotine-containing botanical synergist compound insecticide does not contain any chemical pesticide component, has low toxicity to people and livestock, is environment-friendly, and is mainly applied to control of aphids such as Aphis citricola vonder Goot, brevicoryne brassicae, macrosiphoniella sanborni, Aphis gossypii Glover and the like on fruit trees, vegetables and flowers and empoasca flavescens on commercial crops such as mulberry, teaplants, peach trees, pear trees, cotton, tobacco, hemps and the like. The nicotine-containing botanical synergist compound insecticide has great application and popularization values of organic agricultural products in the production, and is a novel ideal pollution-free insecticide.

The invention discloses a device and method for annual propagation of cotton aphid apple. The device is an insect rack, and an angle is formed by intersecting wooden boards I and II, and a cultivation plastic cup is placed on the angle; the cotton aphid is parasitized Apple seedlings and healthy apple seedlings were transplanted into cultivation plastic cups respectively, and the height and angle of the inoculation device were adjusted according to the height of healthy apple seedlings and apple seedlings parasitized with apple aphid, so as to ensure that healthy apple seedlings could reach The apple seedlings parasitic with the apple aphid are then completed the work of inoculation. The invention can realize the annual planting and propagation of apple seedlings, thereby realizing the annual propagation of the apple cotton aphid without being limited by time, and overcomes the method that only 3-4 months can be transplanted to obtain apple seedlings in production; The damage to aphids caused by inoculation with a writing brush greatly improved the success rate of inoculation. At the same time, the apple cotton aphid was easy to colonize on young apple seedlings, and the standardized test insects were bred to speed up the progress of the experiment.

The invention discloses a method and a kit applied to sensitive aphis gossypii glover detection and identification in field cotton planting. The method comprises the following steps: obtaining sensitive strain aphis gossypii glover; diluting a pesticide by 0.05% TritonX-100; obtaining reagent diagnosis concentration of 50 mg / L and 60 mg / L; taking twice dosage of the diagnosis concentration as distinguishing dosage, wherein the distinguishing dosages of acetamiprid and imidacloprid are 100 mg / L and 120 mg / L respectively; selecting a glass test tube of which the length is 7 cm, and the aperture is 1.5 cm; rolling membrane in the glass test tube to prepare a pesticide selecting kit, wherein the inner surface area of the test tube is 27.5 cm<2>, and the pesticide dosage of the tube wall is 0.44 micrograms / cm<2> and 0.53 micrograms / cm<2>; sealing the opening with silica gel to prepare the testing box. Aphis gossypii glover in cotton field is placed in during test; sensitive aphis gossypii glover screening and sensitivity identification links are eliminated; the detection is simple and quick; safety classification is conducted on toxicity and sensitivity; favorable application value is provided.

The invention relates to a pesticide combination for prevention and treatment of aphis gossypii glover and a preparation method thereof, and belongs to the technical field of pesticide. The pesticide combination comprises a spraying agent and a root-irrigation agent which are combined for use. The spraying agent is prepared from aloes, ailanthus altissima leaves, safflower, quisqualis indica, dichroa febrifuga lour and sucrose fatty acid ester. The root-irrigation agent is prepared from diethyl aminoethyl hexanoate, garlic oil, KH2PO4, glucoheptonic acid, phosphatidyl ethanolamine, polyethylene glycol 200 and herb residues obtained after the spraying agent is extracted. According to the pesticide combination, a Chinese traditional medicine extracting solution is used for killing the aphis gossypii glover with poison, the prevention and control effect is good, environmental friendliness is achieved, low toxicity is achieved, the quantity of residues is small, pesticide resistance of the aphis gossypii glover is not prone to be achieved, and thus the pesticide combination is ideal green pesticide; the spraying agent is combined with the root-irrigation agent for use, nutrients needed by crops can be supplemented while the aphis gossypii glover is killed with poison, growth and development of the crops are promoted, and hence the yield is increased; the spraying agent can dissolve honeydew defecated by the aphis gossypii glover, mold breeding is reduced, the influence on the crops by the aphis gossypii glover is eliminated completely, and the growth vigor of the crops is recovered.