8 results about "Aphis gossypii" patented technology

The invention discloses a dsRNA nano nucleic acid insecticide for preventing and treating piercing-sucking pests, the dsRNA nano nucleic acid insecticide is composed of dsRNA of a specific targeting piercing-sucking pest Chs gene and a carrier, the piercing-sucking pests are Panonychus citri, Diaphorina citri or Aphis gossypii, the nucleotide sequences of the Chs gene are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and the carrier is MgAl or MgFe layered double-metal hydroxide nanoparticles. The dsRNA nano nucleic acid insecticide is applied to host plants of the piercing-sucking pests, after the plants induce the piercing-sucking pests to eat the dsRNA nano nucleic acid insecticide, RNA synthesis and silent Chs genes are interfered in vivo, death is promoted, and the dsRNA nano nucleic acid insecticide has good application prospects in treatment of the piercing-sucking pests.

The invention discloses a method for breeding aphidius gifuensis, which adopts a specific breeding process, adopts specific male parent and female parent domestication with staged humidity and temperature and adopts different subculture schemes to construct a specific aphidius gifuensis breeding method, and aims to solve the problems that the existing aphidius gifuensis is easy to cause hyperparasitism when the number of cotton aphids is low, and the breeding efficiency is low. The cotton aphid is massively propagated, and the prevention and treatment effect is poor. According to the technical scheme, the breeding mode that the female parents and the male parents are alternately subjected to low-temperature domestication has a remarkable influence on the parasitism rate of F5-generation individuals obtained through breeding, compared with a control group, the parasitism rate difference of the aphidius gifuensis is remarkable, and the survival rate in low-temperature feeding and high-temperature feeding is also remarkably influenced; the stress resistance of the aphidius gifuensis can be improved, and the green and sustainable development of the cotton industry can be realized.

The invention belongs to the technical field of artificial reproduction of natural enemy insects and biological control of agricultural pests, and discloses a method for mixed feeding of two kinds of aphid parasitic wasps. The method is mainly technically characterized by comprising the following steps: breeding host plants, inoculating and breeding aphids, obtaining and inoculating two kinds of aphid parasitic wasps, collecting the parasitic wasps, removing impurities, and separating the two kinds of parasitic wasps. According to the method for mixed feeding of the two kinds of aphid parasitic wasps, the two kinds of parasitic natural enemies of the aphidius gifuensis and the aphis gossypii are inoculated in different time periods, the parasitic efficiency of the single species of wasps is improved, interspecific competition between adult wasps is avoided, the two kinds of parasitic wasps are collected at the same time, and manpower and material resources are saved.

The invention relates to the technical field of insect genetic engineering, in particular to a cotton aphid salivary protein gene C002 and application thereof. The nucleotide sequence of the separated cotton aphid C002 gene is as shown in SEQ ID NO: 1, and the sequence of the protein coded by the cotton aphid C002 gene is as shown in SEQ ID NO: 2. The invention further provides application of the cotton aphid C002 gene RNA interference sequence. The sequence has a good silencing effect on the cotton aphid C002 gene. According to the C002 interference sequence-transfected VIGS cotton plant, the number and weight of offspring of cotton aphid are remarkably reduced, and the population development can be effectively controlled. The gene and the protein provided by the invention can be used for development of transgenic insect-resistant plants and biological prevention and control of aphis gossypii glover.

The invention relates to an AgDoa gene and application thereof in reduction of insecticide resistance of cotton aphid, and belongs to the technical field of biological genetic engineering, the AgDoa gene has a DNA sequence as shown in SEQ ID No: 1, and consists of 1782 nucleotides; the amino acid sequence of the protein coded by the AgDoa gene is shown as SEQ ID No: 2, and the protein consists of 593 amino acids; the AgDoa gene can be used as a target to develop a novel insecticide; the AgDoa gene is knocked down, so that the tolerance of the aphis gossypii glover to the insecticide is reduced, and the aphis gossypii glover can be applied to drug resistance treatment and novel pesticide development.

A method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii is provided, relating to the technical field of cotton safety evaluation. The method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii includes planting the gene-edited low-gossypol cotton indoors, collecting young leaves and inoculating nymphal Aphis gossypii on the leaves, rearing the nymphal Aphis gossypii, recording a number of adult Aphis gossypii deaths and a number of newly hatched nymphal Aphis gossypii, calculating an Aphis gossypii mortality rate and an average Aphis gossypii productivity, and evaluating the influence.

A new and distinct melon variety NUN 71560 MEM is disclosed as well as seeds and plants and fruits thereof. NUN 71560 MEM is a Honey Dew melon variety, comprising resistance to Fusarium oxysporum f. sp. melonis Race 0 and Race 2, Podosphaera xanthii Race 5, and Aphis gossypii.

This invention belongs to the field of fruit tree resistance gene application technology, and relates to an apple transcription factor MYB79 gene and its application in improving apple resistance to the woolly aphid. The amino acid sequence of the protein encoded by the MYB79 gene is shown in SEQ ID NO:1, and the nucleotide sequence is shown in SEQ ID NO:2. This invention constructs a plant expression vector carrying the MYB79 gene and recombinant Agrobacterium, and obtains MYB79-overexpressing apple plants through Agrobacterium-mediated transformation. Functional verification results show that MYB79 gene overexpression can significantly improve apple resistance to the woolly aphid, reduce the feeding efficiency and survival rate of the woolly aphid, and significantly shorten the feeding time of the woolly aphid in the phloem of the transgenic plants, resulting in a significant decrease in survival rate, while having no significant impact on the normal growth and development of the apple plants. This invention provides new gene resources and technical support for molecular breeding of apples resistant to the woolly aphid and innovation of insect-resistant germplasm.