A method for tissue culture and rapid propagation of Calamus chinensis

A technique for rapid propagation of iris calamus and tissue culture, which is applied to horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve the problem of planting cost, increased harvesting cost of wild iris calamus, high planting cost of iris calamus, and drug use. issues such as increased costs

Active Publication Date: 2021-11-16
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are very few researches on the artificial seedling cultivation and planting of Acorus calamus, which leads to the cultivation of Acorus calamus according to the traditional planting method, resulting in higher planting costs and a large demand for seedlings. The cost and the harvesting cost of wild calamus calamus have increased significantly, resulting in an increase in the cost of medication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Inoculate the stem tip of Iris calamus in the callus induction medium, and culture it in the dark for 40-50 days to obtain the callus;

[0016] The callus is transferred to the differentiation and proliferation medium, and the differentiation and proliferation is cultured for 30-40 days to obtain adventitious buds;

[0017] The adventitious buds are cut into individual seedlings, and the individual seedlings are inoculated in the medium for rooting and strong seedlings, rooted and cultivated for 25 days, domesticated, and obtained;

[0018] The callus induction medium is MS+6-BA 1.0mg / L+TDZ 0.1mg / L+2,4-D 0.1mg / L+sucrose 30g / L+agar powder 5g / L, the pH is 5.8-6.0;

[0019] The differentiation and proliferation medium is MS+6-BA 0.5mg / L+NAA 0.1mg / L+sucrose 30g / L+agar powder 5g / L, pH5.8-6.0;

[0020] Rooting and strong seedling medium is 1 / 2MS+NAA 0.1mg / L+IBA 0.1mg / L+GA3 0.1mg / L+sucrose 30g / L+agar powder 5g / L, pH5.8-6.0.

Embodiment 2

[0022] On the basis of Example 1, after the callus is formed in the callus culture medium, it is first inoculated in the embryogenic callus induction medium and cultivated for 10-30 days, and then inoculated in the differentiation and proliferation medium for cultivation; The embryogenic callus induction medium is MS+6-BA 0.5mg / L+TDZ 0.1mg / L+NAA 0.1mg / L+sucrose 30g / L+fructose 5g / L+hydrolyzed peptone 300g / L+agar powder 5g / L, pH 5.8-6.0. Others are all the same as in Example 1.

Embodiment 3

[0024] On the basis of Example 2, no sucrose, fructose, and hydrolyzed peptone components were added to the embryogenic callus induction medium, and other components and dosages were unchanged, and other contents were the same as in Example 2.

[0025] To embodiment 1-3 tissue culture process, form the formation rate of callus to shoot tip, callus differentiation forms the formation rate of adventitious bud, and its result is as shown in table 1 below:

[0026] Table 1

[0027] Callus formation rate (%) Adventitious bud formation rate (%) Example 1 87.4 90.4 Example 2 86.9 93.5 Example 3 87.5 79.7

[0028] The data shown in Table 1 shows that for the culture of embryogenic callus induction medium, it helps to promote the formation of adventitious buds, and can improve the adventitious bud formation rate to a certain extent; for the composition of ingredients and ingredients in the medium, it will be The formation rate of adventitious buds...

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PUM

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Abstract

The invention relates to the technical field of seedling raising of Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of calamus calamus. The stem tip of calamus calamus is used as a raw material for tissue culture and rapid propagation, and undergoes callus induction culture, differentiation and proliferation culture, rooting and strong seedlings Cultivation has realized the tissue culture seedling of Iris calamus, accelerated the cultivation of Iris calamus seedlings, met the seedling demand in the planting process of Iris calamus, and provided a feasible plan for preserving the germplasm resources of Iris calamus.

Description

technical field [0001] The invention relates to the technical field of raising seedlings of traditional Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of calamus calamus. Background technique [0002] Nine-section calamus is the dried rhizome of Anemone altaica Fisch.exe C.A.May., a plant of Ranunculaceae (Ranunculaceae), which contains saponins, flavonoids and other ingredients. One of the commonly used traditional Chinese medicine. At present, there are many medicinal and scientific researches on calamus calamus, and the amount of use has increased significantly, resulting in a serious shortage of supply in the existing market. Therefore, it is necessary to increase the artificial seedling cultivation and planting of calamus calamus. But, in the prior art, for the relevant research of calamus calamus, mainly focus on the application of calamus calamus and the extraction of active ingredients in calamus calamus, for example:...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘红美梁大勇石建龙
Owner 遵义市龙驰生物科技有限公司
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