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A root-knot nematode and knot-nematode technology is applied to the field of the three-layer diseased soil inoculation method for potted plant root-knot nematode nematodes, which can solve the problems of early onset of potted seedlings, death of seedlings, and large amount of inoculated diseased soil, and achieves high inoculation success rate, The effect of increased population density and reduced workload
Inactive Publication Date: 2018-10-19
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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Problems solved by technology
However, the existing root-knot nematode single disease soil inoculation technology still has the following problems to be solved: the amount of inoculation disease soil is large, the inoculated potted seedlings have early onset, and some tests have not been completed, and the seedlings have died of disease
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Embodiment 1
[0037] Tomato root-knot nematode potted 3-layer diseased soil inoculation test, including the following steps:
[0038] (1) Collection and treatment of disease-free soil
[0039] Collect the root-knot nematode-free pond soil, add decomposed pig manure organic fertilizer after crushing, and mix well to obtain disease-free soil for later use. The volume ratio of decomposed pig manure organic fertilizer and root-knot nematode-free pond soil is 1:10 ..
[0040] (2) Sick soil collection and treatment
[0041] Collect the diseased roots and rhizosphere diseased soil with root-knot symptoms in tomato roots, chop the diseased roots into fragments of about 1 cm, add the diseased root fragments into the rhizosphere diseased soil and mix well to obtain the diseased soil for later use.
[0042] (3) Propagation and cultivation of diseased soil
[0043] A. Seedling cultivation: Put the commercially available special seedling substrate into a 50-hole seedling pot, sow the commercially ava...
Embodiment 2
[0064] Melon root-knot nematode potted 3-layer disease soil inoculation test, including the following steps:
[0065] (1) Collection and treatment of disease-free soil
[0066] The root-knot nematode-free wasteland soil was collected and crushed, then added with decomposed chicken manure organic fertilizer, and mixed well to obtain disease-free soil for later use. The volume ratio of decomposed chicken manure organic fertilizer and root-knot nematode-free wasteland soil was 1:10.
[0067] (2) Sick soil collection and treatment
[0068] Collect diseased roots and rhizosphere diseased soil with root-knot symptoms at the roots of melons, chop the diseased roots into 1-2 cm fragments, add the diseased root fragments into the rhizosphere diseased soil and mix well to obtain root-knot nematode diseased soil for later use.
[0069] (3) Propagation and cultivation of diseased soil
[0070] A. Seedling cultivation: Put the commercially available special seedling substrate into a 50-h...
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Abstract
The invention belongs to the technical field of plant root-knot nematode inoculation methods, and particularly provides a plant root-knot nematode potting three-layer sick soil inoculation method. Themethod comprises the steps of 1, disease-free soil collection and treatment; 2, sick soil collection and treatment; 3, sick soil expanding propagation cultivation; 4, expanded propagation sick soil pretreatment; and 5, three-layer sick soil inoculation. The plant root-knot nematode potting three-layer sick soil inoculation method adopts a three-layer sick soil inoculation technology that disease-free soil is inoculated on upper and lower layers, and nematode sick soil is inoculated on a middle layer. Compared with an existing root-knot nematode egg and larvae inoculation technology, the technology approaches natural inoculation, the inoculation survival rate is high, a nematode selecting link is omitted, and the workload is greatly reduced. Compared with an existing root-knot nematode single sick soil inoculation technology, the technology has the advantages that the usage amount of the sick soil is reduced by 1 / 2-2 / 3, and the workload and cost of the collection, transportation, expanding propagation cultivation and the like of the sick soil are greatly reduced; and newly transplanted seedlings can be protected from being infected by nematodes, not only can the success of the inoculation be ensured, but also too early disease death of the seedlings can be avoided.
Description
technical field [0001] The invention belongs to the technical field of plant root-knot nematode inoculation methods, and specifically provides a plant root-knot nematode inoculation method with three layers of diseased soil in potted plants. Background technique [0002] Root-knot nematodes are soil-borne diseases of plants. In recent years, with the contiguous planting of susceptible hosts, the transfer of diseased seedlings, the expansion of planting area in protected areas, and the increase of multiple cropping index, root-knot nematode disease has generally occurred and aggravated year by year. More than 50%, or even no harvest, has become an important disease in production. [0003] In the study of root-knot nematode control, pot experiments such as biological control of root-knot nematode, chemical control, resistance identification, and pesticide screening are carried out, and whether these pot experiments can be carried out smoothly depends on whether the root-knot ...
Claims
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