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Classical swine fever virus auxiliary fluid for inoculating rabbit and preparation method thereof

A technology of swine fever virus and auxiliary liquid, which is applied in the direction of antiviral agents, medical preparations containing active ingredients, and pharmaceutical formulas, which can solve problems such as unsatisfactory immune effects, improper use methods, and uneven quality, and achieve improved Inoculation success rate, reduction of inoculation loss, and the effect of ensuring stability

Inactive Publication Date: 2015-09-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the market believes that the immunization effect of the live vaccine of classical swine fever spleen and lymph is not ideal, and there are still misunderstandings about the live vaccine of classical swine fever and spleen. Excessive stress and uneven quality

Method used

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  • Classical swine fever virus auxiliary fluid for inoculating rabbit and preparation method thereof
  • Classical swine fever virus auxiliary fluid for inoculating rabbit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A kind of auxiliary solution of swine fever virus for inoculating rabbits, the virus dilution solution contains 3g of amino acid combination (0.3g of methionine, 0.6g of lysine, 0.5g of tryptophan, 0.6g of glutamic acid in every 1000ml of phosphate buffer solution) , glycine 0.6g and alanine 0.4g), vitamin combination 6g (including vitamin A 1.2g, vitamin D 1.6g, vitamin E 1.4g, vitamin K 1.8), compound Chinese medicine polysaccharide 10g, sodium selenite 3mg, the auxiliary The liquid pH was 7.4.

[0045] A preparation method for swine fever virus auxiliary liquid for inoculating rabbits, the steps are:

[0046] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml ...

Embodiment 2

[0056] A kind of auxiliary solution of swine fever virus for inoculating rabbits, the virus dilution solution per 1000ml phosphate buffer (made of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05g, 0.05g of magnesium chloride containing 6 crystal waters dissolved in 1000ml of double distilled water) contains: 4g of amino acid combination (including 0.5g of methionine, 0.8g of lysine, 0.4g of tryptophan, 0.8g of glutamic acid, Glycine 0.9g and alanine 0.6g), vitamin combination 8g (including vitamin A 1.4g, vitamin D 2.4g, vitamin E 1.6g, vitamin K 2.6g), compound Chinese medicine polysaccharide 15g (composed of 20 parts of Yunzhi, carrageenan Algae 10 parts, gastrodia elata 10 parts, peony 5 parts and peach kernel 5 parts), sodium selenite 5mg, the pH of the auxiliary solution is 7.5.

[0057] A kind of preparation method of swine fever virus auxiliary solution for inoculating rabbits, its...

Embodiment 3

[0059] A kind of auxiliary solution of swine fever virus for inoculating rabbits, the virus dilution solution per 1000ml phosphate buffer (made of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g, chloride Calcium 0.2g, magnesium chloride 0.2g containing 6 crystal waters dissolved in 1000ml double distilled water) contains: Amino acid combination 5g (including methionine 0.6g, lysine 1.2g, tryptophan 0.8g, glutamic acid 0.6g , glycine 0.6g and alanine 1.2g), vitamin combination 10g (including vitamin A 1.0g, vitamin D 3.0g, vitamin E 2.8g, vitamin K 3.2g), compound Chinese medicine polysaccharide 30g, sodium selenite 10mg, the The pH of the auxiliary solution is 7.6.

[0060] A kind of preparation method of swine fever virus auxiliary solution for inoculating rabbits, its steps are the same as embodiment 1.

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Abstract

The invention discloses a classical swine fever virus auxiliary fluid for inoculating a rabbit and a preparation method thereof, and belongs to the technical field of biological products for livestock. Every 1000ml of phosphate buffer solutions in the auxiliary fluid comprises the following components in proportions by weight: 6g to 10g of vitamin compositions, 3g to 5g of amino acid compositions, 10g to 30g of compound Chinese medicinal herbal polysaccharides, and 0 to 10mg of sodium selenite. The classical swine fever virus auxiliary fluid for inoculating the rabbit has the positive and beneficial effects that a virus can be delivered to a susceptible organ when the rabbit is inoculated; the inoculation loss is reduced; the inoculation success rate is improved; the infection stability of the rabbit can be improved; batch-to-batch differences are reduced; the batch-to-batch stability of finished-product vaccines is ensured; the quality of the vaccines is improved; the multiplication density of a classical swine fever virus in the body of the rabbit can be specifically improved; in comparison with the prior art, the titer of the virus is increased by 5 to 10 times; the weight of a gained splenic organ is discovered to be slightly increased after the rabbit is inoculated; in comparison with the prior art, the weight of the gained splenic organ is increased by 15 percent to 40 percent.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a swine fever virus auxiliary solution for inoculating rabbits and a preparation method thereof. Background technique [0002] Classical swine fever (CSF) is a Class A legal infectious disease of the International Organization for Animal Health (OIE) and China. It is one of the major animal diseases that are subject to compulsory immunization in my country, and is the "first killer" that endangers my country's pig industry. Swine fever is caused by swine fever virus and cannot be controlled with drugs. Vaccination is the most effective way. [0003] At present, the CSF vaccines on the market at home and abroad mainly include CSF splenic vaccine, CSF cell vaccine and CSF subunit vaccine. As a classical-refined live vaccine of classical swine fever and splenic gonorrhea in vaccines, it still has an irreplaceable role. [0004] The Chinese swine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8988A61K31/715A61K39/39A61P31/14A61P37/04A61K31/198A61K31/405A61K31/07A61K31/59A61K31/05A61K31/355
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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