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Sugarcane detoxication tissue culturing and fast propagating method

A technology for tissue culture and rapid propagation and sugar cane, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of pollution, low survival rate, and cannot completely remove viruses, and achieves recovery of yield and quality, and detoxification. Simple and effective solution to the problem of sugarcane germplasm degradation

Inactive Publication Date: 2008-03-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem with this shoot apical meristem detoxification method is that it is difficult to cut out very small shoot apical meristems below 0.2mm, and it needs to be operated under a microscope; The lower the rate, the larger the cut shoot tip tissue, the virus cannot be completely removed, and it is easy to cause pollution; therefore, there is an urgent need for a better method in production to meet the needs of sugarcane virus-free tissue culture and rapid propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 (the terminal bud of sugarcane is explant)

[0029] 1) Selection and sterilization of explants: From June to November, take the terminal buds of sugarcane, soak them in 75% alcohol for 0.5-1.0min, then sterilize them with 0.1% mercuric chloride solution for 10min, and finally wash them with sterile water. Rinse 3 to 5 times, as explants for detoxification tissue culture;

[0030] 2) Basic medium: use MS basic medium, sugar 20g / L, agar 9g / L, pH5.6;

[0031] 3) Callus induction culture: Under aseptic conditions, 2-3mm shoot tip tissue was extracted from the explant after sterilization in step (1), and inoculated in MS+2, 4-D 1mg / L+NAA 0.1 On the callus induction medium of mg / L, until induction forms callus;

[0032] 4) Differentiation culture: transplant the callus induced in step (3) to the differentiation medium of MS+6-BA 2.0mg / L+NAA 0.1mg / L to induce cluster buds;

[0033] 5) Proliferation culture: Cut the cluster buds differentiated in step (4) into i...

Embodiment 2

[0037] Embodiment 2: (with the axillary bud of sugarcane as explant 1)

[0038] 1) Selection and sterilization of explants: In November, axillary buds of sugarcane were taken, soaked in 75% alcohol for 0.5-1.0 min, then sterilized with 0.1% mercuric chloride solution for 15 min, and finally rinsed with sterile water for 3-5 min. Second, as explants for virus-free tissue culture;

[0039] 2) Basic medium: use MS basic medium, sugar 25g / L, agar 8g / L, pH5.7;

[0040] 3) Callus induction culture: Under aseptic conditions, 2-3mm shoot tip tissue was extracted from the explant after sterilization in step (1), and inoculated in MS+2, 4-D 2mg / L+NAA 0.2 On the callus induction medium of mg / L, induce the formation of callus;

[0041] 4) Differentiation culture: transplant the callus induced in step (3) to the differentiation medium of MS+6-BA 3.0mg / L+NAA 0.3mg / L to induce cluster buds;

[0042] 5) Proliferation culture: Cut the cluster buds differentiated in step (4) into individual ...

Embodiment 3

[0046] Embodiment 3: (with the axillary bud of sugarcane as explant 2)

[0047] 1) Selection and sterilization of explants: In November, axillary buds of sugarcane were taken, soaked in 75% alcohol for 0.5-1.0 min, then sterilized with 0.1% mercuric chloride solution for 10 min, and finally rinsed with sterile water for 3-5 min. Second, as explants for virus-free tissue culture;

[0048]2) Basic medium: use MS basic medium, sugar 30g / L, agar 7g / L, pH5.8;

[0049] 3) Callus induction culture: Under aseptic conditions, 2-3 mm of shoot tip tissue was extracted from the explant after sterilization in step (1), and inoculated in MS+2, 4-D 1.5mg / L+NAA 0.15mg / L callus induction medium to induce callus formation;

[0050] 4) Differentiation culture: transplant the callus induced in step (3) to the differentiation medium of MS+6-BA 2.5mg / L+NAA 0.15mg / L to induce cluster buds;

[0051] 5) Proliferation culture: Cut the cluster buds differentiated in step (4) into individual plants an...

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Abstract

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

Description

technical field [0001] The invention relates to the technical field of plant detoxification tissue culture rapid propagation, in particular to a method for sugarcane detoxification tissue culture rapid propagation. Background technique [0002] Sugarcane mosaic disease has been widely occurring in Zhejiang and Fujian provinces for many years. The new leaves show light yellow mottled or slight chlorotic stripes, and the old leaves show long chlorotic stripes or mosaic leaves. The incidence is very high, causing sugar and yield. decline (about 18%), and the caste also declines, affecting the quality. [0003] There are many ways to obtain virus-free plants, such as heat treatment detoxification, shoot tip culture detoxification and shoot tip micrografting detoxification and combination methods. Wherein the better method that generally adopts is shoot tip culture detoxification method, and concrete steps are: cut shoot tip (0.2mm following meristem) through plant top meristem,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 徐刚汪一婷牟豪杰吕永平陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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