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Method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems

A pathogenic, basidiomycete technology, applied in the field of plant protection, can solve the problems of troublesome in vitro operation and low success rate

Inactive Publication Date: 2018-08-17
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a method for measuring the pathogenicity of pathogenic wood-rot fungi in basidiomycetes by using the stems of living trees, which solves the problems of troublesome in vitro operation and low success rate of existing inoculation methods

Method used

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  • Method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems
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  • Method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment one: a kind of method utilizing living standing tree stem to measure the pathogenicity of areca stalk rot pathogen (Ganoderma boninensePat.) comprises the following steps:

[0025] 1. Making culture medium

[0026] Sawdust culture medium: mix 82% wood chips, 15% wheat bran, 2% glucose and 1% gypsum according to weight percentage, add water to moisten, mix evenly and pack into small plastic bags with a diameter of 3cm and a height of about 5cm, and seal them , sterilized by moist heat at 121°C for 1h.

[0027] 2. Make the inoculation rod

[0028] Pick 2 mycelium pieces of 1cm × 1cm size from the colony of the betel nut stem rot pathogen that was purified and cultured, inoculate them into the sterilized sawdust medium, and place them at 26°C for 10 days to make the inoculum, to be inoculated The hyphae in the body are covered with bacteria bags and used as inoculation rods for subsequent use.

[0029] 3. Vaccination

[0030] In the season (May) that is suit...

Embodiment 2

[0033] Embodiment two: a kind of method utilizing living standing tree stem to measure willow white rot pathogen [Funalia trogii (Berk.) Bondartsev & Singer] pathogenicity, comprises the following steps:

[0034] 1. Making culture medium

[0035] Sawdust culture medium: mix 82% wood chips, 15% wheat bran, 2% glucose and 1% gypsum according to weight percentage, add water to moisten, mix evenly and pack into small plastic bags with a diameter of 6cm and a height of about 10cm, and seal them , sterilized by moist heat at 121°C for 2h.

[0036] 2. Make the inoculation rod

[0037] Inoculate 5mL of the purified and cultured willow white rot pathogen liquid into the sterilized sawdust medium, and culture it at 28°C for 40 days to make the inoculum. Stick spare.

[0038] 3. Vaccination

[0039] In the season suitable for germ infection (September), choose a 3-year-old healthy willow tree, first use 70% alcohol to spray the surface of the stem within 30cm of the young tree on the...

Embodiment 3

[0042] Embodiment three: a kind of method utilizing living standing tree stem to measure the pathogenicity of rubber tree red root pathogen, comprises the following steps:

[0043] 1. Making culture medium

[0044] Sawdust culture medium: mix 82% wood chips, 15% wheat bran, 2% glucose and 1% gypsum according to weight percentage, add water to moisten, mix evenly and pack into small plastic bags with a diameter of 6cm and a height of about 10cm, and seal them , sterilized by moist heat at 121°C for 2h.

[0045] 2. Make the inoculation rod

[0046] Inoculate 5mL of the purified and cultured liquid for Hevea rubra root pathogen into the sterilized sawdust medium, and culture it at 28°C for 40 days to make the inoculum. spare.

[0047] 3. Vaccination

[0048] In the season suitable for germ infection (September), choose a healthy rubber tree that has grown for 3 years, first use 70% alcohol to spray the surface of the stem within 30cm above the ground of the young tree, and af...

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Abstract

The invention discloses a method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems. The method includes the steps: (1) manufacturing a wood dust culture medium containing 82% of wood dust, 15% of wheat bran, 2% of glucose and 1% of gypsum; (2) introducing bacterial strains into the wood dust culture medium and manufacturing an inoculant stick; (3) selecting a young tree, disinfecting the young tree, cutting a stem 10-30cm away from the ground to form a 1-5cm long and 1-5mm deep notch serving as an inoculation position matched with a shear mouth on one side of the inoculant stick, attaching wet cotton to the lower end of the inoculation position to preserve moisture, fixedly wrapping the inoculation position with a plastic strip, and wrapping the stem about 5-10cm away from the upper portion of the inoculation position with thick paper to form an umbrella to cover the inoculant stick; (4) performing inoculation and infection for 1-2 months, unfastening the thick paper and the plastic strip wrapping an inoculant and verifying the pathogenicity. By the method, the young standing timber stems are inoculated for the first time, the pathogenicity of the pathogen wood-rotting fungi causing tree stem rot and root rot is measured, success rate is high, and operation is simple.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a method for inoculating pathogenic wood rot fungi in basidiomycetes by using the stems of living standing trees and its pathogenicity verification application. Background technique [0002] Wood-decaying fungi can degrade rotten wood and fallen wood, most of which are Basidiomycota fungi, some of which can infect the stem or root system of standing trees in forests, causing stem rot or root rot of trees , affect the growth of trees, and in severe cases, the whole plant will die. It is an important pathogenic wood rot fungus. For tree stem rot or root rot caused by pathogenic wood-rotting fungi, the determination of pathogenicity is a big problem in the process of completing Koch's law to verify its pathogenicity. At present, most of them use artificial inoculation of isolated materials to complete the determination of the pathogenicity of tree stem rot or root rot caused by wood...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18G01N2333/375
Inventor 李增平张宇丁婧钰吴如慧
Owner HAINAN UNIVERSITY
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