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A kind of large-scale propagation and cultivation method of nematode stinkii

A cultivation method and a large-scale technology, applied in the directions of nematicides, botanical equipment and methods, insecticides, etc., can solve the problems of low reproduction yield, single insecticidal scope, and high cost, and achieve simple reproduction and cultivation methods and prevention and control. A wide range of pests and the effect of improving the success rate

Active Publication Date: 2017-07-25
北京安和亿泰生物工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a large-scale breeding and cultivating method of sternerne elegans, which solves the problems of long cycle, high cost, low activity and low reproductive output of sternerne elegans in the prior art, and also solves the problems of The problem of low insecticidal rate and single insecticidal range of bred nematode stinkii

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] According to the content provided by the present invention, the inventor bred and bred the entomophagous nematode 1, and the bred and bred method is as follows:

[0023] Inoculate the nematode Steineria elegans into the body of the edible meat worm, the culture temperature is 22°C, the relative humidity of the culture is 70%, and the pH value of the mist water used for humidity control is 4.5; after 220 hours of cultivation, the inoculated Add a formaldehyde solution with a weight concentration of 6‰ to the successful edible meat worms of stinkii nematode, completely soak the edible meat worms successfully inoculated with stigmangi nematode in the formaldehyde solution, and place it for 15 minutes; pour it into a centrifuge tube, The separation temperature was 28° C., and the entomophagous nematode 1 was isolated, and 100,000 entomophilic nematodes 1 were produced from each carnivorous worm.

Embodiment 2

[0025] According to the content provided by the present invention, the inventor bred and bred the entomophagous nematode 2, and the bred and bred method is as follows:

[0026] Inoculate the nematode Steineria elegans into the meat worm, the culture temperature is 24°C, the relative humidity of the culture is 80%, and the pH value of the mist water used for humidity control is 4.6; after 260 hours of cultivation, the inoculated A formaldehyde solution with a weight concentration of 7‰ was added to the successful edible meat worms of stinkii nematodes, and the successful edible meat worms inoculated with the small toritrix nematode stignerii were completely soaked in the formaldehyde solution, and placed for 18 minutes; poured into a centrifuge tube, The separation temperature was 28° C., and the entomophagous nematode 2 was isolated, and 80,000 entomophagous nematodes 2 were produced from each carnivorous worm.

Embodiment 3

[0028] According to the content provided by the present invention, the inventor bred and bred the entomophagous nematode 3, and the bred and bred method is as follows:

[0029] Inoculate the nematode Steineria elegans into the body of the edible meat worm, the culture temperature is 26°C, the relative humidity of the culture is 90%, and the pH value of the fog water used for humidity control is 4.5; after 240 hours of cultivation, the inoculated A formaldehyde solution with a weight concentration of 8‰ was added to the successful edible meat worms of stinkii nematodes, and the successful edible meat worms inoculated with stigmangi nematodes were completely soaked in the formaldehyde solution, and placed for 20 minutes; poured into a centrifuge tube, The separation temperature was 28°C, and the entomophagous nematodes 3 were separated, and 90,000 entomophagous nematodes 3 were produced from each carnivorous worm.

[0030] The inventor used the obtained nematode 1, nematode 2 an...

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Abstract

The invention discloses a steinernema batch breeding and culture method, which comprises the following steps that steinernema is inoculated into edible worms, the culture temperature is 22 to 26 DEG C, the relative humidity in the culture process is 70 to 90 percent, and the pH value of the fog water for humidity control is 4.5 to 4.6; after the culture for 220 to 260 houses, formaldehyde solution with the weight concentration being 0.6 to 0.8 percent is added into the edible worms successfully inoculated with the steinernema, and the edible worms successfully inoculated with the steinernema is completely soaked into the formaldehyde solution to be placed for 15 to 20 minutes; the solution is poured into a centrifugal pipe, the separation temperature is 28 DEG C, and the liposcelis steinernema is separated out. The edible worms are used for breeding and culturing the steinernema, the breeding and the culture method is simple, the steinernema inoculation success ratio is improved through strictly controlling the inoculation quantity of each edible warm, and the solution containing formaldehyde is added into the culture process of the steinernema, so that the yield of the liposcelis steinernema is exponentially increased. The cultured liposcelis steinernema carries bacilli containing biopharmaceutin, and the insecticidal rate is greatly improved and is as high as more than 95 percent.

Description

technical field [0001] The invention relates to the field of reproduction and cultivation of nematode stinkii, in particular to a method for mass reproduction and cultivation of nematode stikrina. Background technique [0002] At present, people mainly use pesticides to prevent and control various diseases. As an ideal biopesticide, Steiner's nematode has the following advantages: it is obligate to parasitize herbivorous insect larvae and has a wide range of hosts; Search ability, can be cultivated in large quantities with artificial medium at low cost; it can be mixed with many chemical pesticides when applied in the field, and it is safe for humans, animals, plants, beneficial organisms and the environment. Since the nematode stikrina is an environmentally friendly biological natural enemy, the cost of reproduction and cultivation is low. [0003] At present, the reproductive yield of the nematode stikrilii is low, and the insecticidal rate of the nematode stikrilii produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01N63/02A01P5/00A01P7/04
Inventor 易闻石长安
Owner 北京安和亿泰生物工程技术有限公司
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