Molecular marker related with wheat thousand grain weight and applications thereof

A thousand-grain weight, wheat technology, applied in the field of molecular markers, can solve the problems of high chance of polymorphism

Inactive Publication Date: 2015-02-11
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

In addition, since many restriction endonucleases can digest the amplified DNA, there is a greater chance of detecting polymorphisms

Method used

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  • Molecular marker related with wheat thousand grain weight and applications thereof
  • Molecular marker related with wheat thousand grain weight and applications thereof
  • Molecular marker related with wheat thousand grain weight and applications thereof

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Embodiment 1

[0030] Example 1, 2 SNPs related to wheat thousand-grain weight and PCR-RFLP polymorphism detection thereof

[0031] 1. Specific primers and sequence analysis for amplifying a genomic DNA fragment containing 2 SNPs in wheat

[0032] Two SNPs were found in the gene 6-SFT (Genbank No. FJ228688) on the wheat genome, corresponding to the 1371th position from the 5' end of the sequence table sequence 1 (named X site, with G and A polymorphisms) sex), and the 2450th position from the 5' end of Sequence Listing Sequence 1 (named Y site, there are G and A polymorphisms); these two sites X and Y have three types in the natural variation population of wheat Monotype:

[0033] Single type A: G, G;

[0034] Haplotype B: G, A;

[0035] Monotype C: A, G;

[0036] According to the sequence differences of different genomes of wheat, specific primers were designed to PCR amplify the DNA fragments including these two SNP sites:

[0037] F: 5'-CTCTCTAGACATAATCAAAAGGGA-3' (SEQ ID NO: 2);

...

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Abstract

The invention discloses a molecular marker related with wheat thousand grain weight and applications thereof. Through the heritable variation analysis on 6-SFT gene of a wheat natural variation group, people find that the 6-SFT gene has two SNPs corresponding to the 1371st and 2450th positions in the tail ends from 1 to 5' in the sequence one. Three haplotypes exist in the two SNP, namely haplotype A (G,G) haplotype B (G,A), and haplotype C (A,G). Through correlation analysis, the wheat thousand grain weights of the three homozygous haplotypes are accord with a relationship as follows: the wheat thousand grain weight of homozygous haplotype C > the wheat thousand grain weight of homozygous haplotype B > the wheat thousand grain weight of homozygous haplotype C. The invention also provided a CAPS label for detecting the two SNPs. Experiments have proved that the wheat with a higher thousand grain weight can be found through detecting the two SNPs. A novel method is provided for the wheat breeding assisted by a molecular labeling technology, and the provided molecular maker has an important meaning for the breeding and researches of wheat species with a high yield.

Description

technical field [0001] The invention relates to a molecular marker related to wheat thousand-grain weight and its application. Background technique [0002] my country is the largest country in wheat production and consumption in the world, and wheat production is closely related to national food security. Drought has always been the most important abiotic stress factor affecting wheat production. Fructan is not only an important storage soluble carbohydrate, an important carbon source for grain filling, but also a major osmotic adjustment substance. The accumulation of fructan in plants can improve their ability to resist drought and cold. Therefore, the research and utilization of wheat fructan synthase (Sucrose:fructan6-fructosyltransferase, 6-SFT) gene is of great significance for the genetic improvement of high yield and drought resistance. Wheat 6-SFT is one of the key enzymes in the process of fructan synthesis. It has two functions. One is to directly use sucrose a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 景蕊莲岳爱琴李昂毛新国昌小平
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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