Corn gene ZmRAVL1 and functional site and application thereof

Abstract

The invention relates to a corn gene ZmRAVL1 and a functional site and application thereof. According to the method, the functional site controlling angle between leaf and stem phenotype is positionedto 240 bp through fine mapping, and different angle between leaf and stem phenotypes are generated through insertion or deletion of the region. The invention proves that the corn yield can be increased under high planting density by utilizing the excellent natural variation improved inbred line from Teosinte, and the sources of available excellent alleles in plant breeding are broadened. The invention proves that the reduction of the expression of ZmRAVL1 can influence plant type, for example, the reduction of angle between leaf and stem by a genetic engineering technology (RNAi), and provides an excellent gene resource for genetic engineering breeding. More excellent alleles are generated by the invention through a gene editing technology, so that the selection process of the excellent alleles is greatly shortened, and a new thought for obtaining available excellent alleles in breeding practice is provided. By combining a molecular assisted selection technology, an excellent inbred line can be rapidly and accurately improved or generated, and the invention provide the possibility for wide application of excellent alleles.

Description

technical field [0001] The invention belongs to the field of plant gene map-based cloning and molecular breeding, and specifically relates to a corn gene ZmRAVL1 and related functional sites and corresponding applications. Background technique [0002] Corn is the world's largest food crop, and now the world's total corn production has exceeded 1 billion tons. In China, as in the United States, corn is mainly used as feed. However, China's corn planting area is similar to that of the United States, but its per unit yield and total production are about 60% of that of the United States. The growth space of my country's corn planting area is close to the limit, and what is more serious is that the harsh natural environment such as water shortage and frequent extreme weather is irreversible. There is very limited room for increasing maize production in my country simply by increasing the sown area. The fundamental way out is to fully tap the huge potential of maize genetic res...

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Problems solved by technology

[0008] In summary, although some progress has been made in the genetic mapping of maize leaf angle, and breakthroughs have been made in the cloning of individual leaf angle genes, the research on the entire maize leaf angle is still mainly at the preliminary location. Horizontally, the genetic and molecular regulatory network established by maize leaf angle is far from elucidated

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