Corn gene ZmRAVL1 and functional site and application thereof

A kind of application and gene technology, applied in the field of maize gene ZmRAVL1 and related functional sites, can solve the problems that the research of maize leaf angle is still at the level of preliminary positioning, and the genetic and molecular regulation network of maize leaf angle is far from elucidated.

Active Publication Date: 2020-12-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In summary, although some progress has been made in the genetic mapping of maize leaf angle, and breakthroughs have been made in the cloning of individual leaf angle genes, the researc

Method used

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  • Corn gene ZmRAVL1 and functional site and application thereof
  • Corn gene ZmRAVL1 and functional site and application thereof
  • Corn gene ZmRAVL1 and functional site and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Initial and fine positioning of the ZmRAVL1 gene

[0092] Using a set of RIL populations constructed by backcrossing and selfing the inbred line W22 of maize and the ancestral species - teosinte CIMMYT 8759 (abbreviated as 8759 in this paper), the QTL of leaf angle was initially mapped. The phenotypic value of the angle between the angle and the flag leaf was blurred and then used as the phenotype input value for QTL mapping, combined with 19378 high-density and high-quality molecular markers, using the multi-QTL model in R / qtl, using R software (Version 3.1. 0) Carry out QTL mapping. After 1000 times of permutation test (Permutation test) (significance P=0.01), it was determined that the QTL significance thresholds of the angle between the upper leaf on the panicle and the angle between the flag leaf were both LOD=5.

[0093] For corn ear upper leaf angle, a total of 10 QTLs were mapped, among which UPA2 (Compact Plant Architecture 2) located on chromosome 2...

Embodiment 2

[0124] Example 2 Nucleic acid molecular information of ZmRAVL1

[0125] The nucleic acid molecular sequence of ZmRAVL1 was obtained on maizeGDB (https: / / www.maizegdb.org / ) (because the genome sequence of the maize inbred line B73 and the genome sequences of several other maize inbred lines have been determined, only the nucleic acid sequence As far as most genes are concerned, the sequences of most genes are known, and the sequences of all genes including this gene can be obtained from multiple websites such as maizeGDB; although the function of this gene can be predicted on websites such as maizeGDB, but The function of this gene regulating the angle of corn leaves was discovered by the inventor for the first time. The materials used in the present invention are corn inbred line W22 and corn wild species Teosinte 8759, W22 has completed sequencing, and the genome sequence has also been released; The sequence of the gene in Teosinte 8759 was obtained by referring to the genome...

Embodiment 3

[0129] Example 3 Protein molecular information of ZmRAVL1

[0130] (1) Sequence encoding protein

[0131] ( https: / / www.maizegdb.org / gene_center / gene / GRMZM2G102059 )

[0132] MEFASSSSRFSREEDEEEEQEEEEEEEEASPREIPFMTAAATADTGAAASSSSPSAAASSGPAAAPRSSDGAGASGSGGGGSDDVQVIEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDAAANEKGQLLSFEDRAGKLWRFRYSYWNSSQSYVMTKGWSRFVKEKRLDAGDTVSFCRGAGDTARDRLFIDWKRRADSRDPHRMPRLPLPMAPVASPYGPWGGGGGGGAGGFFMPPAPPATLYEHHRFRQALDFRNINAAAAPARQLLFFGSAGMPPRASMPQQQQPPPPPHPPLHSIMLVQPSPAPPTASVPMLLDSVPLVNSPTAASKRVRLFGVNLDNPQPGTSAESSQDANALSLRTPGWQRPGPLRFFESPQRGAESSAASSPSSSSSSKREAHSSLDLDL

[0133] (SEQ ID No: 27) (see image 3 )

[0134] (2) Domains of encoded proteins

[0135] (http: / / smart.embl-heidelberg.de / smart / show_motifs.pl)

[0136] The structural domain prediction of the amino acid sequence encoded by the gene in the Smart website shows that the amino acid sequence encoded by the gene contains a B3 domain ( image 3 Amino acid sequences marked in red). In the gramene web...

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Abstract

The invention relates to a corn gene ZmRAVL1 and a functional site and application thereof. According to the method, the functional site controlling angle between leaf and stem phenotype is positionedto 240 bp through fine mapping, and different angle between leaf and stem phenotypes are generated through insertion or deletion of the region. The invention proves that the corn yield can be increased under high planting density by utilizing the excellent natural variation improved inbred line from Teosinte, and the sources of available excellent alleles in plant breeding are broadened. The invention proves that the reduction of the expression of ZmRAVL1 can influence plant type, for example, the reduction of angle between leaf and stem by a genetic engineering technology (RNAi), and provides an excellent gene resource for genetic engineering breeding. More excellent alleles are generated by the invention through a gene editing technology, so that the selection process of the excellent alleles is greatly shortened, and a new thought for obtaining available excellent alleles in breeding practice is provided. By combining a molecular assisted selection technology, an excellent inbred line can be rapidly and accurately improved or generated, and the invention provide the possibility for wide application of excellent alleles.

Description

technical field [0001] The invention belongs to the field of plant gene map-based cloning and molecular breeding, and specifically relates to a corn gene ZmRAVL1 and related functional sites and corresponding applications. Background technique [0002] Corn is the world's largest food crop, and now the world's total corn production has exceeded 1 billion tons. In China, as in the United States, corn is mainly used as feed. However, China's corn planting area is similar to that of the United States, but its per unit yield and total production are about 60% of that of the United States. The growth space of my country's corn planting area is close to the limit, and what is more serious is that the harsh natural environment such as water shortage and frequent extreme weather is irreversible. There is very limited room for increasing maize production in my country simply by increasing the sown area. The fundamental way out is to fully tap the huge potential of maize genetic res...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/113A01H5/00A01H6/46
CPCC07K14/415C12N15/8218C12N15/8213C12N15/8261C12N15/113C12N2310/10C12N2310/20C12Q1/6895C12Q2600/13C12N15/8262Y02A40/146
Inventor 田丰田金歌王成龙
Owner CHINA AGRI UNIV
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