Drug detection kit

Abstract

The invention provides a drug detection kit and its preparation process, which combines highly specific antibody antigen reaction with immune colloidal gold chromatography technology, improves on the basis of this prior art, and detects antibody in colloidal gold labeling At the same time, mark the control line antibody, and correspondingly point the second antibody on the membrane at the quality control area (C line) for color reaction with the colloidal gold-labeled control line antibody, so as to ensure that the C line will always develop color, and the color will develop The degree will not change, so it can be judged whether one or more drugs are contained by observing whether the detection area (T line) is colored, and one or more drugs can be semi-quantitatively compared by observing the degree of color development of the T line . The drug detection kit has the characteristics of qualitative and semi-quantitative comparison of drugs, and also has the characteristics of rapidity, sensitivity, easy operation, low cost, and no need for professional detection.

Description

technical field [0001] The invention relates to the technical field of biology and new medicine, in particular to a drug joint detection kit, which is suitable for screening in drug rehabilitation centers, hospitals, military conscription, high-risk population censuses, special industries and physical examinations for recruitment, and food inspections by health and epidemic prevention departments. Wait. Background technique [0002] At present, the gold standard method is mainly used for urine testing in drug testing, which is characterized by fast, convenient, portable, and high accuracy. The commonly used methods for the determination of single drugs in urine are: monoclonal antibody monolithic qualitative method, thin-layer chromatography, gas chromatography, and gas-mass spectrometry detection method, but these methods exist to varying degrees. Cross-reaction, many interference factors and complex pre-treatment process are insufficient. Gas-mass spectrometry (GC-MS) de...

AI Technical Summary

Problems solved by technology

However, the existing drug detection test cards can only detect the presence of drugs qualitatively, and cannot achieve quantitative or semi-quantitative detection, or can achieve quantitative detection of drugs, but the process is extremely complicated and the cost is high

[0005] In the prior art, the multi-drug compound detection method is to combine highly specific antibody-antigen reaction with immunocolloidal gold chromatography technology, but the defect is that the detection test paper is affected by the color intensity of the C-line during the compound detection. The influence of the color intensity makes it impossible to semi-quantitatively compare the detection results of different antigens although the presence of multiple drugs in the same urine sample can be detected

In addition, the defect of the prior art drug detection test paper is that the color intensity of the C-line is affected by the color intensity of the T-line, which must be one dark and one shallow, and the function of the C-line is only to judge whether the chromatography reaches the control area. However, if the C line does not develop color, there may be other situations besides the invalid test. For example, when the drug content in the test sample exceeds the detection threshold, the colloidal gold-labeled antibody is completely reacted, resulting in the detection being effective but the C line is still ineffective. Color rendering