Cloned enzyme-donor immunoassay (CEDIA) ImmunoChip drug detecting kit

Abstract

Rapid drug detection is a regular detection item for public security departments, drug control organizations, sports events, enlistment and entry departments. At present, colloidal gold test paper is a basic choice for rapid drug detection, but the sensitivity of the colloidal gold test paper is low, the possibility of fake positive and fake negative results is high, the repeatability is low and the quantification is impossible. The invention discloses a cloned enzyme-donor immunoassay (CEDIA) ImmunoChip drug detecting kit. The kit comprises an immuno chip on which the combination of an enzyme donor and an enzyme receptor is immobilized, wherein the enzyme donor can be coupled with a drug micromolecule by a chemical bond to form an enzyme-donor-labeled exogenous antigen so as to be used for detecting whether a specific anti-drug antibody which can be competitively combined with the enzyme-donor-labeled exogenous antigen exists in a sample to be detected by a CEDIA ImmunoChip drug detecting method and calibration is carried out by using a novel fluorogenic substrate. The kit has the advantages of sensitive reaction, high accuracy, no cross reaction and high repeatability, and is simple and convenient to operate.

Description

technical field [0001] The invention relates to a detection kit, in particular to a CEDIA ImmunoChip drug detection kit. Background technique [0002] Drug rapid detection is a detection item that public security departments, anti-drug agencies, sports events, military enlistment, and entry departments often need to apply. At present, the products on the market are relatively single, and the production units are also very limited. Colloidal gold test paper is the basic choice for rapid drug detection at present, but the sensitivity of colloidal gold test paper is not high, there are high false positive and false negative results, and the repeatability is low. also cannot be quantified. [0003] Drug detection mainly uses chemical (chromatographic chromatography) or biological (antigen-antibody reaction) methods to detect drugs and their metabolites in human urine, hair, and blood. At present, there are mainly the following methods: [0004] Simple test strip detection: th...

AI Technical Summary

Problems solved by technology

[0004] Simple test strip detection: the principle is to use drug colloidal gold and the antibody binding site with limited competition in urine. For reference, positive samples need to be further tested by chemical methods to confirm

[0005] Gas chromatography (GC / MS) detection: the use of gas chromatography to detect drugs in urine has the advantage of being accurate and sensitive, and is the most reliable method so far. The disadvantage is that the operation is complicated, the sample needs to be processed in advance, and the requirements for instrument operation are high. The detection time is long and the cost is high, so it is not suitable for screening

[0006] ELISA detection: The principle is to use the drug in the urine to react with the labeled antibody, and use the corresponding instrument to read the signal. There are many methods, such as Cloned enzyme donor immunoassay (CEDIA), enzymes multiplied immunoassay technique (EMIT) And kinetic interaction of microparticle in solution (KIMS), the advantage is that it is fast, and the sensitivity and specificity are significantly improved compared with the test paper, but the disadvantage is that it still needs to be further tested to determine

[0007] The above methods have their own insurmountable defects, so there is an urgent need for a new on-site detection method that is cheaper, simpler, faster, accurate, reliable and quantitative to replace the existing detection method