A femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification

Abstract

The present invention provides a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification, including: a laser light source and a beam splitter; a sample excitation light generation part, which is used for frequency conversion of the fundamental frequency light output by the laser light source; The focusing device of the sample excitation light and the sample cell where the sample is fixed; the collection and convergence system of the sample fluorescence; the generation part of the pump light required for the fluorescence light parametric amplification; the optical parametric crystal, used for the non-collinear light generation of the pump light and the fluorescence Parametric process; time-resolved fluorescence spectrum data acquisition system; optical path delay system, used to change the time delay between optical parametric pump light and sample excitation light; data acquisition system is a data acquisition system based on a multi-channel lock-in amplifier. The invention can realize a single measurement to obtain a time-resolved fluorescence spectrum without hyperfluorescence background interference.

Description

technical field [0001] The invention belongs to the technical field of femtosecond time-resolved fluorescence spectrum measurement. Specifically, the invention relates to a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification. Background technique [0002] Time-resolved fluorescence spectroscopy is an important technical method in the fields of photophysics, photochemistry, and biology. It is used to obtain information such as excited state lifetime, energy or electron transfer, and structural changes in the system. The current methods for realizing femtosecond time-resolved fluorescence spectroscopy include fluorescence up-conversion technology, optical Kerman technology, and femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy technology. Compared with the former two, femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectrosc...

AI Technical Summary

Problems solved by technology

However, there are differences in the shape of the hyperfluorescence spectrum between adjacent optical parametric pump light pulses, and only when the energy amplification of the fluorescence photon satisfies the small signal approximation, the energy amplification of the fluorescence photon will not cause the change of the hyperfluorescence spectrum shape

Second, the intensity of the hyperfluorescence spectrum recorded alone differs from that recorded in the presence of fluorescence photon energy amplification

Therefore, the above method of step-by-step measurement cannot accurately subtract the interference of the hyperfluorescent background on the time-resolved fluorescence spectrum

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