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A femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification

A technology of optical parameter amplification and fluorescence spectrometer, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of hyperfluorescence spectrum difference, hyperfluorescence spectrum intensity difference, and inability to accurately deduct hyperfluorescence background, etc., to achieve compact structure, The effect of reducing sample photodamage, improving dynamic range and detection sensitivity

Active Publication Date: 2016-08-17
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

However, there are differences in the shape of the hyperfluorescence spectrum between adjacent optical parametric pump light pulses, and only when the energy amplification of the fluorescence photon satisfies the small signal approximation, the energy amplification of the fluorescence photon will not cause the change of the hyperfluorescence spectrum shape
Second, the intensity of the hyperfluorescence spectrum recorded alone differs from that recorded in the presence of fluorescence photon energy amplification
Therefore, the above method of step-by-step measurement cannot accurately subtract the interference of the hyperfluorescent background on the time-resolved fluorescence spectrum

Method used

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  • A femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification
  • A femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification
  • A femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification

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Embodiment 1

[0032] The present invention provides as attached figure 2 The shown femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification mainly includes the following parts:

[0033] 1) The laser light source 201 is a titanium-doped sapphire femtosecond regeneration amplification system (central wavelength around 800nm, pulse repetition frequency 1kHz, single pulse energy greater than 300uJ, pulse width about 100fs).

[0034] 2) The light beam splitter 202 has a transmission and reflection ratio of 1:1 for the 800nm ​​beam, and is used to divide the laser output from the laser light source 1 into a transmitted beam and a reflected beam.

[0035] 3) The sample excitation light part is located in the optical path of the above-mentioned transmitted light beam, including a non-collinear optical parametric amplifier (NOPA) 203, a frequency doubler (SHG) 204 and an optical filter 205, wherein the non-collinear optical parametric ...

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Abstract

The present invention provides a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification, including: a laser light source and a beam splitter; a sample excitation light generation part, which is used for frequency conversion of the fundamental frequency light output by the laser light source; The focusing device of the sample excitation light and the sample cell where the sample is fixed; the collection and convergence system of the sample fluorescence; the generation part of the pump light required for the fluorescence light parametric amplification; the optical parametric crystal, used for the non-collinear light generation of the pump light and the fluorescence Parametric process; time-resolved fluorescence spectrum data acquisition system; optical path delay system, used to change the time delay between optical parametric pump light and sample excitation light; data acquisition system is a data acquisition system based on a multi-channel lock-in amplifier. The invention can realize a single measurement to obtain a time-resolved fluorescence spectrum without hyperfluorescence background interference.

Description

technical field [0001] The invention belongs to the technical field of femtosecond time-resolved fluorescence spectrum measurement. Specifically, the invention relates to a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification. Background technique [0002] Time-resolved fluorescence spectroscopy is an important technical method in the fields of photophysics, photochemistry, and biology. It is used to obtain information such as excited state lifetime, energy or electron transfer, and structural changes in the system. The current methods for realizing femtosecond time-resolved fluorescence spectroscopy include fluorescence up-conversion technology, optical Kerman technology, and femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy technology. Compared with the former two, femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectrosc...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 翁羽翔党伟王专于清旭陈珂毛鹏程王云鹏
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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