Method for extracting bacterium genomic DNA by using magnetic nanoparticles

A magnetic nanoparticle and genome technology, applied in the field of nanomaterials and molecular biology, can solve the problems of long time consumption, low extraction efficiency, and use of toxic reagents, and achieve the effect of short time consumption, high extraction efficiency and simple operation
CN102121002AInactive Publication Date: 2011-07-13JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2011-07-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for extracting bacterium genomic DNA by using magnetic nanoparticles, belonging to the technical field of nanomaterials and molecular biology. The method comprises the steps of: preparing a magnetic nanoparticle aggregate, preparing a bacterium lysing solution, preparing and adding an absorption buffer solution, absorbing with a magnet, rinsing with 70 percent alcohol, and eluting with a TE buffer solution to obtain a bacterium genomic DNA solution. Compared with the traditional phenol / chloroform DNA extracting method, the method for extracting the bacterium genomic DNA by using magnetic nanoparticles has the advantages of short experiment time, simpleness in operation, high extraction efficiency, rapidness, convenience, sensitivity, high yield and the like; and the extracted bacterium genomic DNA can be completely used for subsequent experiments, such as DNA hybridization, PCR (Polymerase Chain Reaction), and the like, and provides the basis for the subsequent research and analysis.
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Description

technical field

[0001] The invention relates to a method for extracting bacterial genome DNA by using magnetic nanoparticles, belonging to the technical fields of nanomaterials and molecular biology. Background technique

[0002] Bacteria are widely distributed in nature, and some pathogenic microorganisms can invade human body and animals, cause infection or even infectious disease, and pose great harm to human and animal health. Eating food contaminated with these bacteria and drinking water contaminated with bacteria are the main routes of transmission of foodborne diseases. According to statistics, at least 200,000 to 400,000 people actually suffer from food poisoning every year in my country. In recent years, food poisoning has increased year by year. There are many pathogenic microorganisms that cause food poisoning, the most common being Vibrio enteritidis, Salmonella, Campylobacter and pathogenic coliform bacteria.

[0003] The traditional method of bacterial dete...

Claims

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