Method for extracting bacterium genomic DNA by using magnetic nanoparticles
A magnetic nanoparticle and genome technology, applied in the field of nanomaterials and molecular biology, can solve the problems of long time consumption, low extraction efficiency, and use of toxic reagents, and achieve the effect of short time consumption, high extraction efficiency and simple operation
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[0030] (1) Preparation of magnetic nanoparticle aggregates:
[0031] ①
[0032] Weigh 0.65g of anhydrous ferric trichloride and 0.4g of trisodium citrate, measure 20ml of ethylene glycol, and stir for 20min to dissolve them completely;
[0033] ② Weigh 1.2g of anhydrous sodium acetate and add it to the above reaction system, stir for 10min, put it in a tetrafluoroethylene reactor and heat it to 230°C in an oil bath, and at the same time carry out magnetic stirring, stop heating after 10h of reaction, continue magnetic stirring to cool to room temperature ;
[0034] ③Add 20ml of absolute ethanol to the reacted solution, ultrasonically clean it for 10min, centrifuge at 5000rpm for 10min, and discard the supernatant;
[0035] ④ Add 20ml of absolute ethanol to the lower layer, ultrasonically clean for 10min, centrifuge at 5000rpm for 10min, and discard the supernatant;
[0036] ⑤ Add 20ml of deionized water to the lower sediment, ultrasonically clean for 10min, centrifuge at 50...
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