Mammal oocyte quality evaluation method based on optical property detection

Abstract

The invention provides a mammal oocyte quality evaluation method based on optical property detection. The method comprises the following steps: oocytes are collected from a mammal, and the oocytes donot need any biochemical treatment; a Mueller matrix type full-field OCT system is combined with a Mueller matrix decomposition and transformation technology to represent the polarization characteristics of each biological structure of oocytes; a full-field OCT system is combined with a Fourier analysis technology to characterize backscattering spectrum characteristics of all biological structures of oocytes; and the backscattering intensity characteristics of each biological structure of the oocyte are characterized by analyzing the gray scale distribution characteristics and the frequency spectrum characteristics of the OCT image of the oocyte. The method has the characteristics of no damage, no labeling, high resolution, three-dimensional chromatography, simplicity in operation, shortexperimental time, easiness in implementation and the like, and can be used for effectively evaluating the development quality and development potential of the mammal oocytes.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for evaluating the quality of mammalian oocytes based on optical property detection. Background technique [0002] In recent years, due to factors such as environmental pollution, delayed childbearing age, and increased life pressure, the number of infertile families has increased year by year. Infertility has become a global reproductive health problem, seriously affecting patients' physical and mental health, family stability and social function. The most commonly used and most recognized method for treating infertility is in vitro fertilization-embryo transfer and its derivatization (IVF-ET-D). During the IVF-ET-D process, two cell screenings are involved, namely the screening of high-quality oocytes during in vitro fertilization and the screening of high-quality embryos during embryo transfer. Among them, the quality of oocytes is an important factor to determi...

AI Technical Summary

Problems solved by technology

However, the traditional morphology-based detection method has the problem that the reliability of the results depends on the experience of technicians, and the results of morphology screening are too subjective, which seriously affects the success rate of assisted reproduction

Biotechnology detection takes a long time (ranging from several hours to tens of hours), which is very unfavorable for oocyte quality evaluation

Because the obtained quality evaluation results can only represent the developmental state of the oocyte several hours or tens of hours ago, and after a long biological test, there is a very likely risk that the quality of the oocyte has deteriorated

It can be seen that the complicated experimental steps and time-consuming are the bottlenecks for evaluating the quality of oocytes based on biological techniques

Although the research on oocyte quality screening based on spectroscopic technology has great clinical application prospects, there are still common problems such as poor in situ performance and lack of high-resolution three-dimensional tomography capabilities, resulting in the use of these methods for oocyte quality screening. The results are not ideal, and cannot efficiently distinguish oocytes of different developmental qualities

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