95results about How to "Avoid Fluorescence Quenching" patented technology

The invention relates to a rare earth hydrotalcite-like compound/polymer nanocomposite and a preparation method thereof. The method comprises the following steps: firstly prefabricating: dissolving sodium metaaluminate and sodium hydroxide in deionized water to prepare a solution A, dissolving magnesium chloride in deionized water to prepare a solution B, dissolving rare earth oxide with dilute hydrochloric acid and then putting them into the solution B to prepare a solution C, and dissolving a single ligand or a mixed ligand in sodium stearate to prepare a mixed slurry D; then under the action of strong stirring or ultrasonic wave, simultaneously adding the solution A and the solution C dropwisely in the mixed slurry D to prepare a mixed slurry E, aging, conducting pumping filtration, drying the filter cake to obtain a bright fluorescent rare earth hydrotalcite-like compound; and immersing the rare earth hydrotalcite-like compound in a polymer monomer and initiator for 24 hours, and then conducting polymerization to obtain the nanocomposite. The materials disclosed herein has excellent luminescence property of original rare earth complex, and has the advantages of good formability, strong impact resistance, light weight, low cost, easiness in film formation and the like of organic polymer materials.

The invention discloses a process for preparing a high-performance sensing film of a fluorescent sensor by covalently immobilizing an indicator dye. The process comprises the following steps: cleaning a quartz glass plate sequentially with 3% HF acid, 10% H2O2 and distilled water; introducing thiohydroxy on the surface of the cleaned glass plate by (3-mercaptopropyl)trimethoxysilane (MPS); self-assembling the glass plate with the thiohydroxy in gold nanoparticle colloidal solution for 12-24h (preferred 15h) and fixing the gold nanoparticles on the glass plate; immersing the glass plate on which the gold nanoparticles are fixed in ethanol solution of (1.0-5.0)*10<-3>mol L<-1> (preferred 2.0*10<-3>mol L<-1>) mercapto carboxylic acid for 10-18h (preferred 12h), introducing long carbon chains and carboxyl on the surfaces of the gold nanoparticles, wherein, the carbon chain of the adopted mercapto carboxylic acid comprises preferably 10-16 carbon atoms; activating the carboxyl on the surfaces of the gold nanoparticles with 1-ethyl-3-(3-dimethylamino propyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS); and immersing the glass plate on which the carboxyl is activated in acetone solution (2.0*10<-4>-5.0*10<-3>mol L<-1> of the indicator dye RNH2 with primary amido, preferred 1.0*10<-3>mol L<-1>) for 4.0-9.0h (preferred 6h), then the indicator dye is covalently immobilized on the outmost layer of the glass plate to form the ultrathin sensing film, then preparing the fluorescent sensor with the covalently immobilized indicator dye. The sensing film of the sensor has the advantages of no dye loss, quick response (response time is less than 2s), easy regeneration and good reversibility when in use.

The invention relates to a preparation method of multifunctional nanometer composite microspheres and concretely relates to a magnetic fluorescent composite nanometer biological probe and a preparation method thereof. The nanometer composite microsphere is prepared by connecting carboxymethyl chitosan-modified magnetic nanoparticles and water soluble quantum dots through crosslinking effects of a cross-linking agent based on a natural organic polymer as a matrix, wherein the fluorescent quantum dots are distributed on the surface of the magnetic nanoparticle, the size of the magnetic nanoparticle is in a range of 10-200nm and the size of the quantum dot is in a range of 1.5-10nm, and after surface functional group modification, the nanometer composite particles can be connected to a biomolecule so that a bioprobe is formed. The preparation method allows mild reaction conditions and has simple processes. The prepared magnetic fluorescent composite nanometer particles have good magnetic properties and fluorescence properties and have a wide application prospect in the fields of biomarking, bioseparation, immunodetection, targeting release of drugs and target-oriented imaging.

The invention discloses a green quantum dot film and a preparation method of a backlight module thereof. The method performs functional design on the protective layer of the green quantum dot film; the outer surface of the protective layer has a good light diffusion effect after being coated, and inorganic diffusion particles in the coated layer play a role in blocking light in some bands, thus the light damage caused by long-time light irradiation on the quantum dot is slowed down. The invention also provides a using method of the green quantum dot film, the using method matches the green quantum dot film and a blue chip with red phosphor to make a novel LED light source, and the using method improves the stability of the quantum dot film while greatly increasing the color gamut coverageof the LCD.

The invention discloses a polyimide containing porphyrin, and aromatic polyimide with better thermal stability is obtained by using aromatic dicarboxylic anhydride with better thermal stability and aromatic organic diamine as basic framework. The preparation method comprises the following step of carrying out condensation reaction on diamino tetraphenyl porphyrin, the aromatic organic diamine and the aromatic dicarboxylic anhydride to synthesize soluble polyimide containing porphyrin, having the characteristics of simple operation, strong controllability and recyclable use of products. The polyimide containing the porphyrin can be used for preparing a corresponding nano fiber film of the polyimide containing the porphyrin by using an electrostatic spinning method, and the nano fiber film is used for detecting explosive steam under an extreme environment, having advantages of high sensitivity and rapid response.

The invention discloses a two-photon dye contained metal organic frame material for detecting physiological temperature. The metal organic frame material can realize fluorescence temperature detection of all-brand biological transparent windows and has the chemical formula of [M(L)x(G)y].(R)n, wherein M is a metal ion, L is a triangular symmetrical organic ligand containing a tricarboxylate group, G is a solvent molecule, and R represents a dye molecule in a pore. The material is prepared with a solvothermal method and emits visible light in a range of 650-700 nm under excitation of laser being 1000-1064 nm. The two wavelength ranges are located in a second biological window and a first biological window, and the problem of absorption by water can be effectively solved; the material is luminous, sensitive to temperature, good in cycling performance and capable of effectively detecting the physiological temperature. Quantum efficiency and thermal stability of dye after being loaded are obviously improved, stability in water and a PBS (physiological buffer solution) is high, and biocompatibility is good. The material is expected to be applied in the fields of biomedicine and the like.

The invention discloses an organic rare earth europium luminescent polypropylene (PP) fiber and a preparation method thereof. The rare earth europium complex Eu(TTA)3DmPPhen luminescent material close to nanoscale is prepared by firstly preparing a phenanthroline neutral ligand and carrying out coordination on the phenanthroline neutral ligand and rare earth Eu (III) in combination with alpha-thenoyltrifluoroacetone and adopting a method of firstly carrying out titration under atmospheric pressure and secondly carrying out reaction under high pressure. The material emits strong pure red light at 615nm in a 10-5mol/L CH2Cl2 solution. The luminescent fiber is prepared by adopting the complex as a luminescent material and carrying out compound melt spinning on the complex and PP. When the content of the rare earth luminescent material in the fiber is 0.05%, the fiber emits stronger pure red light in a 10-5mol/L dimethyl formamide (DMF) solution and the luminescent position is still at 615nm. The luminescent fiber has the advantages of high color purity and better spinnability.

The invention discloses a preparation method and an application method of a core-shell nanocrystal cell temperature sensor. The core-shell nanocrystal cell temperature sensor is a core-shell nanocrystal cell temperature sensor based on rare earth-doped NaYF4. The preparation steps are as follows: rare earth-doped NaYF4 core-shell nanocrystals are first prepared, and are prepared into a solution after hydrophilization, cells are cultured in the solution, and thereby the core-shell nanocrystal cell temperature sensor is obtained. When the sensor is applied, laser is utilized to activate marked to-be-measured cells, a temperature-dependent upconversion fluorescence spectrum of the marked to-be-measured cells is measured, and a functional relation between luminous intensity and cell temperature is established. By ingeniously designing the core-shell structure nanocrystals, the invention gets temperature sensing characteristics deep into the cellular level, consequently, the resolution of cell temperature measurement is greatly increased, the repeatability is good, and cytotoxicity is low.

The invention relates to a method for synthesizing an ultraviolet-writable fluorine-containing erbium-containing polymer waveguide amplifier material and belongs to the field of synthesis of polymer near-infrared light-emitting materials. According to the method, a fluorine-containing active monomer containing an olefin double bond and an epoxy group, an erbium-containing complex and epoxypropyl methacrylate are subjected to ternary polymerization to prepare a fluorine-containing erbium-containing polymer near-infrared light-emitting material so that the compounding of an erbium ion and the polymer at the molecular level is achieved and the fluorescence quenching caused by uneven concentration of a rare earth complex due to phase separation generated by uneven doping is avoided. Polymer optical waveguide amplifier micro-patterns with stripe structures are prepared by carrying out lithography development on a polymer film obtained by a spin coating through a direct ultraviolet-writing technology, compared with the traditional process, copying processes such as evaporating a metal film layer and ion etching are not needed and thus the process steps are reduced and meanwhile, the cost is decreased and the method is an advantageous method for producing a polymer waveguide amplifier.

The invention belongs to the technical field of biosensors and specifically relates to a fluorescence enhanced type aptamer sensor as well as a preparation method and application thereof. A metal nanostructure is guided into a fluorescent molecular detection system, aptamer for modifying a certain amount of basic groups A is combined with metal nanoparticles through sulfydryl, and plasma resonancegenerated by the metal nanoparticles is utilized to improve fluorophore excitation efficiency; thus, fluorescent radiation amplitude is remarkably improved, and the purpose of amplifying fluorescencesignals is achieved. Meanwhile, the fluorescence enhanced type aptamer sensor which is used for detecting low-concentration target objects is provided by being combined with specificity selection ofaptamer, so that flexibility, accuracy and specificity of analysis detection are ensured, and detection of polyphyly of the target object is also achieved. Meanwhile, the fluorescence enhanced type aptamer sensor has the characteristics of low cost, no pollution and wider detection range.

The invention discloses organic rare earth europium luminescent nylon and a preparation method thereof. The rare earth europium complex Eu(TTA)3DmPPhen luminescent material close to nanoscale is prepared by firstly preparing a phenanthroline neutral ligand and carrying out coordination on the phenanthroline neutral ligand and rare earth Eu (III) in combination with alpha-thenoyltrifluoroacetone and adopting a method of firstly carrying out titration under atmospheric pressure and secondly carrying out reaction under high pressure. The material emits strong pure red light at 615nm in a 10-5mol/L CH2Cl2 solution. The luminescent fiber is prepared by adopting the complex as a luminescent material and carrying out compound melt spinning on the complex and PA6 (polyamide 6). When the content of the rare earth luminescent material in the fiber is 0.08%, the fiber emits stronger pure red light in a 10-5mol/L DMF (dimethyl formamide) solution and the luminescent position is still at 615nm. The luminescent fiber has the advantages of high colour purity and better spinnability.

The invention belongs to the technical field of molecular biology and nucleic acid chemistry, and relates to a CA125 detection kit based on Cu-MOF and application of the CA125 detection kit. The kit is based on the utilization of the fluorescence quenching effect of a metal organic framework material and the strong adsorption capacity of metal MOF and a DNA aptamer, and when the DNA aptamer and the MOF coexist, the DNA aptamer and the MOF are combined, and fluorescence quenching is carried out; and in the presence of the target protein, the DNA aptamer is preferentially combined with the target protein or is replaced from the MOF, and the fluorescence value is recovered. The method comprises the following steps: synthesizing the DNA aptamer specifically aiming at CA125, adding a fluorescence label of Cy5.5, and the sequence of the DNA aptamer being TGCCTTATTACTCTCTCCTGTTAAC-Cy5.5; co-incubating standard proteins with different concentrations, the DNA aptamer and the MOF to obtain a fluorescence recovery standard curve, optimizing a reaction system, obtaining an optimal detection range and detection sensitivity, and obtaining different target protein concentrations according to different protein standard curves and recovery conditions of target proteins with different fluorescence values in the detection system. The detection method is rapid, low in cost and high in sensitivity,and does not need complex amplification and detection instruments.

The invention relates to an aggregation-induced light emission red delay material which is composed of an electron withdrawing group anthraquinone and an electron-donating group carbazole or triphenylamine. The material is represented as the structure formula (I), wherein R1 is the carbazole or triphenylamine and is particularly represented as the following structure. The aggregation-induced light emission red delay material has short synthetic route and simple synthetic method, is prepared from easy-to-obtain raw materials, and is suitable for wide application. A red light device prepared from the material as a luminescent layer has high brightness, high efficiency, and low turn-on voltage, and can be widely applied in the field of organic electroluminescence.

The invention relates to fluorescent microspheres and a preparation method and a fluorescent coding method thereof. The preparation method comprises the following steps: mixing microspheres with a silane reagent and a solvent for activation to obtain activated microspheres, with the silane reagent being at least one of an epoxy silane reagent, a chlorosilane reagent, an isocyanate silane reagent,a butanedioic anhydride silane reagent and a chlorosulfonyl phenyl silane reagent; and carrying out a coupling reaction on the activated microspheres and fluorescent dye in a solvent to obtain the fluorescent microspheres. According to the preparation method, one or more types of fluorescent dye are coupled to surfaces of the activated microspheres, so that one or more different fluorescence signals can be obtained. Meanwhile, the mass-volume ratio of each fluorescent dye is adjusted, so that one or more microspheres with different fluorescence and different intensities can be obtained, namelyfluorescent coded microspheres are obtained. The prepared fluorescent microspheres have the advantages of large fluorescent dye coding space, high fluorescent dye intensity and the like, and the defect that the fluorescence intensity of fluorescent coded microspheres prepared by traditional preparation methods cannot meet the requirement is avoided.

The present invention disclosed a method for fabrication of fluorescence-Raman dual enhanced modal biometal substrate. The method comprises the steps of grinding surface of the substrate with different types of sandpapers to remove an oxide layer and smooth the surface of the substrate; wherein a roughness of the surface is less than 0.1 μm; cleaning the grinded substrate in an ultrasonic bath to remove any impurity; placing the specimen on the stage of an ultrashort laser system; processing the specimen at a certain laser processing parameters by a galvanometer; finally, three-dimensional micro-nano structure is fabricated on the specimen. The technique of the present invention is promising for large-scale commercial application because it is simple and economical, while the enhanced Raman and fluorescence signal is stable and high reproducibility.

The invention belongs to the technical field of materials, and particularly relates to a composite material, a preparation method thereof and a luminescent device. The composite material comprises carbon quantum dots and zinc oxide quantum dots, and the carbon quantum dots and the zinc oxide quantum dots are connected through -O-(X1)Si(OH)-O-(X2)Si(OH)-O-; wherein the X1 and X2 are independently selected from a hydrocarbon group or a hydrocarbon group derivative. The composite material can effectively prevent carbon quantum dots from clustering and causing fluorescence quenching when the carbon quantum dots are in solid powder; meanwhile, when the composite material is used for a light-emitting device, the composite material also has no overlapping of an emission spectrum and an absorptionspectrum, so that the self-absorption phenomenon in the composite material is reduced, and the effect of high fluorescence luminous efficiency is achieved.

The invention discloses a photoluminescent fiber and a preparation method thereof. Quantum dots are used as luminescent particles, high-molecular polymers are used as a spinning substrate, the quantumdots and the high-molecular polymers are blended to prepare a spinning solution, and then the photoluminescent fiber is prepared through a wet spinning process. The photoluminescent fiber prepared bythe method has excellent optical stability and mechanical properties, and has a good application prospect in the fields of clothing, pharmacy, environment, petrochemical industry, anti-counterfeitingindustry, gas sensors, human health monitors and the like. The preparation method provided by the invention is simple to perform, has a wide application range of spinning solutions, and is low in cost and easy to apply to large-scale industrial application.

The invention provides an aminohexose enzyme fluorescent probe and a preparation method and application thereof, and relates to the field of biochemical materials. The aminohexose enzyme fluorescent probe provided by the invention has a structure as shown in a formula I. The aminohexose enzyme fluorescent probe has the advantages of few synthesis steps, simple separation and purification operationand good stability, has aggregation induced luminescence characteristic and strong photobleaching resistance, avoids the defect that traditional fluorescent dye is not suitable for detection at highconcentration or cannot be tracked for a long time due to fluorescence quenching caused by aggregation after entering cells, and can be applied to detection of over-expressed aminohexose enzyme in cancer cells.

The invention relates to a method for preparing functionalized carbon-coated magnetic nanoparticles. The method comprises the following steps: (1) dissolving ferrocene in acetone, and performing ultrasonic stirring so as to form a uniform solution; (2) adding polyacrylic acid into the uniform solution obtained in the step (1), and performing magnetic stirring so as to form a precursor; (3) performing a heated heat-preservation reaction on the precursor obtained in the step (2), performing cooling after the reaction is completed, removing the supernate, performing washing with acetone, and performing magnetic separation, thereby obtaining the functionalized carbon-coated magnetic nanoparticles. Compared with the prior art, the functionalized carbon-coated magnetic Fe3O4 nanoparticles prepared by using the method are synthesized by using a solvent heating method, the prepared magnetic submicrospheres are uniform in particle size, good in stability, free of aggregation among particles, and excellent in magnetic property.

The invention provides a synthesis method of a ZnS/CdZnS/ZnS blue light quantum dot. Firstly, an oil-phase ZnS quantum dot is synthesized by utilizing a zinc source precursor and an S source precursorthrough a high-temperature thermal injection method; then, a ZnS quantum dot nucleus is obtained through purification; next, the ZnS quantum dot nucleus is coated with a CdZnS luminous shell layer and a ZnS inorganic modification layer through an epitaxial growth method; finally, the ZnS/CdZnS/ZnS blue light quantum dot is obtained through purification. According to the synthesis method of the ZnS/CdZnS/ZnS blue light quantum dot, materials with different bands are prepared through changing the thickness of different CdZnS layers, and ideal quantum yield is obtained through regulating the thickness of the luminous layer; the thickness of the middle luminous layer is selected to be synthesized under 300 DEG C, so that the crystal lattice fitness between the ZnS nucleus and the CdZnS luminous layer can be relieved; a CdZnS coherence strain layer can be crystallized well; the fluorescent quantum yield of the quantum dot is obviously improved along with the coating of the ZnS shell layer;meanwhile, flicker can be inhibited, and the quantum dot fluorescent yield is high.

The invention provides a preparation method of fluorescently-enhanced graphene quantum dots, the method is as follows: adding acetonitrile into a graphene quantum dot solution, performing ultrasonic cleaning in an ultrasonic cleaner for 5-10min, then transferring to a high-pressure reaction kettle, heating to 175-185 DEG C, reacting at constant temperature for 11 to 12h, and cooling to the room temperature to obtain fluorescent enhanced graphene quantum dots. According to the preparation method provided by the invention, acetonitrile is taken as the auxiliary intermediate to produce the fluorescence enhanced graphene quantum dots by one-step hydrothermal method, the luminescence stability is improved significantly with high luminescence intensity and quantum yield, at the same time, the fluorescence quenching of graphene quantum dots under the high temperature and high pressure environment is effectively prevented, and the application potential of the graphene quantum dots in the fields of photoelectric conversion, ion detection, sensor and the like is greatly expanded.