Targeted capture sequencing detection method for pathogenic microorganisms of respiratory system

Abstract

The invention discloses a targeted capture sequencing detection method for pathogenic microorganisms of a respiratory system. The method comprises the following steps of: S1, acquiring a genome sequence of the pathogenic microorganisms of the respiratory system, and acquiring a specific gene sequence and a non-specific gene sequence according to the genome sequence; and S2, collecting a real pathogenic sample of the respiratory system, introducing a first mutagenesis mechanism into a half component of the pathogenic sample of the respiratory system to obtain a pathogenic mutagenesis sample of the respiratory system, and constructing a pathogenic microorganism sequencing library based on the pathogenic sample of the respiratory system and the pathogenic mutagenesis sample of the respiratory system. According to the method, a mutation mechanism is introduced into the preparation of the pathogenic microorganism sequencing library to simulate a microorganism mutation process, so that the gene sequence abundance of the microorganisms is increased, a sequencing result of the mutated virus microorganisms is prevented from being false negative, and the sequencing accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a targeted capture sequencing detection method for pathogenic microorganisms in the respiratory system. Background technique [0002] The target region is targeted to be captured and then sequenced, and the DNA fragments of the target gene or genomic region are directional enriched from the whole genome and then sequenced, which greatly reduces the cost and the pressure of subsequent data analysis. Compared with conventional sequencing methods, targeted capture followed by sequencing greatly reduces the cost of sequencing and data analysis, while improving detection sensitivity. At present, it is widely used in the clinical detection of human whole exome, but it is relatively rare in the application of pathogenic microorganisms. [0003] The RT-PCR method in the prior art has the highest sensitivity. The method for sequencing novel coronaviruses and other pathogenic micr...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a targeted capture sequencing detection method for pathogenic microorganisms in the respiratory system, so as to solve the problem that the prior art cannot cope with the capture of microbial variant sequences, resulting in a decrease in the accuracy of targeted capture sequencing and the lack of targeted capture probes. Capturing technical issues with low adaptability and poor versatility

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