Methods for increasing accuracy of nucleic acid sequencing
Patent Information
- Authority / Receiving Office
- US · United States
- Patent Type
- Applications(United States)
- Current Assignee / Owner
- FLUIDIGM CORP
- Publication Date
- 2007-10-18
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
TECHNICAL FIELD OF THE INVENTION
[0001] The invention generally relates to methods for increasing accuracy in nucleic acid synthesis reactions. BACKGROUND OF THE INVENTION
[0002] In vitro nucleic acid synthesis is a foundation of many fundamental research and diagnostic tools, such as nucleic acid amplification and sequencing. In a template-dependent nucleic acid synthesis reaction, the sequential addition of nucleotides is catalyzed by a nucleic acid polymerase. Depending on the template and the nature of the reaction, the nucleic acid polymerase may be a DNA polymerase, an RNA polymerase, or a reverse transcriptase.
[0003] The accuracy of template-dependent nucleic acid synthesis depends in part on the ability of the polymerase to discriminate between complementary and non-complementary nucleotides. Normally, the conformation of the polymerase enzyme favors incorporation of the complementary nucleotide. However, there is still an identifiable rate of misincorporation that depends ...