Drug discovering method based on epigenome and application of drug discovering method

A discovery method and genome technology, applied in the fields of genomics, proteomics, bioinformatics, etc., can solve problems such as investment failure to obtain corresponding returns, and achieve solid theoretical foundation, wide application prospects, and high efficiency.

Active Publication Date: 2019-03-29
武汉百药联科科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such a huge investment has not received a commensurate return

Method used

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  • Drug discovering method based on epigenome and application of drug discovering method
  • Drug discovering method based on epigenome and application of drug discovering method
  • Drug discovering method based on epigenome and application of drug discovering method

Examples

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Embodiment 1

[0050] Example 1 Use the method of the present invention to carry out drug discovery for type Ⅱ diabetes

[0051] The flow chart of the epigenome-based drug discovery method of the present invention is as follows figure 1 shown.

[0052] Using the method of the present invention to carry out drug discovery for type II diabetes comprises the following steps:

[0053] (1) Obtain the DNA methylation chip data of type 2 diabetes patient samples and control samples

[0054] The DNA methylation microarray data (data set ID: GSE34008, Illumina HumanMethylation27 chip) of type 2 diabetes patient samples and control samples were obtained from the GEO database. The data set contains 12 samples of type 2 diabetes patients and 12 samples of normal controls, and the tissue of the samples is whole blood tissue. Delete the probes marked with "NA" in the sample, and get 27,070 DNA methylation probe values ​​for each sample, that is, the β value, representing the degree of DNA methylation a...

Embodiment 2

[0071] Example 2 Drug discovery for atherosclerosis using the method of the present invention

[0072] Drug discovery for atherosclerosis using the method of the present invention comprises the following steps:

[0073] (1) Obtain the DNA methylation chip data of atherosclerosis patient samples and control samples

[0074] The DNA methylation microarray data (data set ID: GSE46394, Illumina HumanMethylation450 chip) of atherosclerosis patient samples and control samples were obtained from the GEO database. The data set contains 34 atherosclerosis patient samples and 15 normal control samples, and the tissue of the sample is carotid artery tissue. Delete the probes marked with "NA" in the sample, and get 473,291 DNA methylation probe values ​​for each sample, that is, the β value, representing the degree of DNA methylation at each site, and then according to the platform (GPL13534) probe The corresponding relationship between the sample data probe name and the gene name. If ...

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Abstract

The invention relates to a drug discovering method based on epigenome and application of the drug discovering method, and belongs to the technical field of biological medicines. The invention starts from the basic principle of drug effects, DNA methylation chip data of a sample of a patient is combined to an intergenic interaction network, key pathogenic genes in a disease morbidity process is excavated by a revised PageRank algorithm, and therefore, potential drugs for treating diseases are found out according to a drug target corresponding relation. The method has the characteristics of solid theoretical basis, easiness in implementation and high efficiency, and has wide application prospect in drug discovery.

Description

technical field [0001] The invention relates to an epigenome-based drug discovery method and application thereof, belonging to the technical field of biomedicine. Background technique [0002] Drug research and development is a process with long cycle, high cost, high risk, fierce competition and high profit. According to statistics, it takes 10-15 years for a new drug to be produced from conception, laboratory lead compound identification, optimization, clinical trials to final marketing, and the research and development costs are as high as more than 800 million US dollars (DiMasi, J.A., Hansen, R.W., and Grabowski, H.G. (2003). The price of innovation: new estimates of drug development costs. J. Health Econ. 22:151-185.), and this cost is still increasing year by year. However, such a huge investment has not received a corresponding return. In 1996, there were 53 FDA-approved drugs, but in 2007 it dropped to 15, a record low (Hughes, B. (2008). 2007 FDA drug approvals: ...

Claims

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Application Information

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IPC IPC(8): G16B15/20G16B20/00G16B50/00G16C20/50
Inventor 张红雨崔泽嘉黄清
Owner 武汉百药联科科技有限公司
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