Nanoparticle based on cisplatin and DNA coordination self-assembly, and preparation method and application of nanoparticle

A nanoparticle and self-assembly technology, applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve poor solubility and drug resistance, limit therapeutic effect and application range and other problems, to achieve the effect of improving solubility and uptake efficiency, improving solubility and biocompatibility, and simple production process

Active Publication Date: 2020-08-11
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0003] Platinum compounds are the most widely used first-line clinical anticancer drugs, because their poor solubility and drug resistance limit the clinical therapeutic effect and application range
There are many reports on the preparation of nano-medicines of platinum-based drugs, but there is no report on the preparation of nano-medicines by selecting DNA with better biocompatibility without material modification or introducing other reagents

Method used

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  • Nanoparticle based on cisplatin and DNA coordination self-assembly, and preparation method and application of nanoparticle
  • Nanoparticle based on cisplatin and DNA coordination self-assembly, and preparation method and application of nanoparticle
  • Nanoparticle based on cisplatin and DNA coordination self-assembly, and preparation method and application of nanoparticle

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preparation example Construction

[0033] The preparation method of nanoparticles based on cisplatin and DNA coordination self-assembly of the present invention comprises the following steps:

[0034] (1), dissolving the DNA palindromic sequence in deionized water to obtain an aqueous DNA solution;

[0035] (2), under the condition of avoiding light, mix cisplatin powder and 5wt% physiological saline to obtain cisplatin solution;

[0036] (3) Add the prefabricated DNA aqueous solution and cisplatin solution into the PCR reaction tube, and react at a constant temperature of 90-95° C. for 2-5 hours to obtain the first mixed solution;

[0037] (4) After the reaction is completed, anneal to 55-65°C, and add a DNA palindromic sequence in the amount equal to that in step (1) to the first mixed solution, and continue the constant temperature reaction for 0.5-2h to obtain the second mixed solution ;

[0038] (5) The second mixed solution is centrifuged, and the obtained precipitate is nanoparticles self-assembled bas...

Embodiment

[0052] The preparation method of nanoparticles based on cisplatin and DNA coordination self-assembly of the present embodiment comprises the following steps:

[0053] (1) Prepare a DNA aqueous solution with a molar concentration of 100 μmol / L: mix the DNA palindromic sequence and deionized water in proportion to obtain a 100 μmol / L DNA aqueous solution;

[0054] (2), the preparation molar concentration is the cisplatin solution of 20mmol / L: take the cisplatin powder of corresponding quality and 5wt% physiological saline and mix in proportion under the dark condition, obtain 20mmol / L cisplatin solution;

[0055] (3) Add 90 μL of DNA aqueous solution with a concentration of 100 μmol / L and 10 μL of cisplatin solution with a concentration of 20 mmol / L in the PCR reaction tube, the total volume is 100 μL, the final concentration of DNA aqueous solution is 90 μmol / L, and the final concentration of cisplatin solution The concentration is 2mmol / L, oscillate and mix evenly, and react a...

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Abstract

The invention provides a nanoparticle based on cisplatin and DNA coordination self-assembly, and a preparation method and application thereof. The preparation method comprises the steps: dissolving cisplatin with normal saline under a high-temperature condition to prepare a cisplatin solution; then mixing DNA palindromic sequences with the cisplatin solution, and conducting a constant-temperaturereaction in a PCR instrument; and after annealing, supplementing an equal amount of DNA palindromic sequences, and continuously carrying out the constant-temperature reaction in the PCR instrument, soas to obtain nanoparticles with uniform shape and size. The DNA palindromic sequence is non-toxic and has good biocompatibility, self-crosslinking growth can occur through sequence design, and a hydrogel layer can be formed on the shell of the nanoparticle to improve the stability of the nanoparticle; meanwhile, cisplatin can be coated at the core of the nanoparticle by the DNA palindromic sequences to finally form the nanoparticle which is good in water solubility and relatively stable; the nanoparticle is simple in production process, environment-friendly in production process and relatively high in drug loading capacity, and has a wide application prospect in the field of drug delivery.

Description

technical field [0001] The invention belongs to the technical field of DNA self-assembly, and specifically relates to a nanoparticle based on cisplatin and DNA coordination self-assembly, a preparation method and application thereof. Background technique [0002] DNA, as a storage tool for genetic information in a living system, is one of the most basic and important substances, and has an important impact on all life reactions and processes. In addition, based on the good biocompatibility, solubility, specific binding and thermodynamic stability of DNA, DNA, as a new functional biomolecule, is widely used in the fields of materials science and nanotechnology. DNA nanostructures can achieve high-precision size and shape control, and express rich surface chemical and biochemical properties, which makes DNA-based self-assembled nanoparticles have unique applications in the fields of chemistry, catalysis, and biomedicine. At present, the construction of DNA nanostructures main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/243A61K9/51A61K47/54A61K45/06A61P35/00
CPCA61K9/5123A61K45/06A61P35/00A61K33/243A61K2300/00
Inventor 宋杰王雨琦孔向阳
Owner SHANGHAI JIAO TONG UNIV
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