49 results about "ORDER PRIMATES" patented technology

The invention discloses application of a substance for detecting the expression level of APOE or MT2 in evaluating the ovarian aging degree. According to the invention, cell and molecular changes related to ovarian aging are explored in spatial resolution by adopting a spatial transcriptome technology, a first non-human primate space-time transcriptome map is established, and potential diagnostic biomarkers and therapeutic targets of age-related human ovarian diseases are disclosed; the method not only promotes the understanding of primate ovarian aging mechanism determinants, but also provides abundant resources for identifying cells and molecular programs which can be used for evaluating ovarian aging and formulating intervention measures for human ovarian aging and related diseases.

The utility model discloses a non-contact drinking water system for non-human primates, which comprises a cage, a main line water supply unit and a branch line water supply unit, the main line water supply unit comprises a constant-pressure water source and a drinking water main pipeline, and the drinking water main pipeline is communicated with the interiors of the constant-pressure water source and the branch line water supply unit; one end of the branch water supply unit is communicated with the drinking water main pipeline, and the other end of the branch water supply unit is communicated with the interior of the cage; a water inlet hole is formed in one side of the cage, and the water inlet hole is communicated with the interior of the branch water supply unit. The non-human primate breeding device can well adapt to the breeding scale, funds and breeding technology of the non-human primates, is simple in structure, low in cost, energy-saving and environment-friendly, and can avoid breeding water supply pollution so as to be beneficial to the physical health of the non-human primates.

A tree-dwelling primate identification method based on thermal infrared and visible light image fusion relates to the technical field of target detection, and comprises the following steps: synchronously acquiring a visible light image and a thermal infrared image in a target sample plot; performing image processing on the visible light image and the thermal infrared image; extracting thermal infrared features in the thermal infrared image and biological features in the visible light image; based on the extracted thermal infrared features and biological features, performing a three-layer matching mechanism of rough matching, static matching and biological constraint; carrying out image fusion on the visible light image and the thermal infrared image; carrying out model training and optimization; and outputting a species identification result. According to the method, adaptive infrared radiation correction enhancement is carried out on the complex inhabiting environment of the tree-dwelling primates in the high-altitude area, then thermal infrared features and visible light features are fused to carry out cross-modal feature three-layer matching and modeling, interference of environmental conditions can be effectively eliminated, the recognition precision is improved, and the recognition efficiency is improved. And a key technical support is provided for physiological monitoring of the tree-dwelling primates.

This invention relates to the field of primate husbandry technology, and more particularly to a primate feeder, comprising a box fixedly mounted on a feeding cage, a movable frame slidably mounted inside the box, multiple feed outlets communicating with the interior of the box surface, baffles symmetrically and rotatably mounted at each of the feed outlets, multiple support blocks fixedly mounted on the movable frame, a feed tray slidably mounted between every two support blocks, and a support plate fixedly mounted between every two support blocks, the support plate having a locking component for locking the feed tray. This invention, through the cooperation of the box, movable frame, support components, support blocks, sliding component, locking component, and feed tray, allows feed to be pre-placed in the feed tray, and then the tray to be pushed into the feeding cage, thus achieving non-contact feeding, effectively preventing the feeder from being attacked by the animal during feeding, and significantly improving safety.

The invention discloses a preparation for improving the anti-stress capacity of primates as well as a preparation method and application of the preparation. The preparation is prepared from the following raw materials in parts by weight: 5 to 15 parts of semen ziziphi spinosae extract, 3 to 8 parts of ganoderma lucidum polysaccharide, 1 to 3 parts of gamma-aminobutyric acid, 2 to 5 parts of vitamin C, 1 to 3 parts of vitamin E, 10 to 20 parts of glucose, 2 to 6 parts of astragaloside and 3 to 7 parts of chamomile extract. The preparation method comprises the steps of extracting, mixing and granulating. Through feed addition, drinking water addition and environment spraying application, the multi-component synergistic stress relieving effect can be achieved, safety and low toxicity are achieved, preparation is easy and convenient, application is flexible, and animal health and catching safety are effectively guaranteed.

Provided is a chemical reprogramming method for obtaining an induced pluripotent stem cell from a somatic cell of a primate. Further provided is a composition containing a chemical inducer that can be used in the method.

This invention relates to the field of molecular marker screening technology, specifically to a SNP combination and method for paternity testing in cynomolgus monkeys. The method provided by this invention utilizes 100 selected specific SNP combinations (SNP1~SNP100) combined with low-depth sequencing for paternity testing in cynomolgus monkeys. The cost per sample is only 1 / 5 to 1 / 10 of existing high-depth sequencing and 1 / 2 of SNP chips. For large-scale experimental monkey populations (such as populations of thousands), this can save hundreds of thousands of yuan in testing costs annually, significantly reducing the economic burden of breeding and management of experimental monkeys, demonstrating extremely high economic efficiency. The method provided by this invention is applicable to cynomolgus monkey population pedigree management, kinship tracing, breeding pair optimization, and genetic quality control, and can be widely applied in experimental monkey breeding farms, primate research centers, and biomedical research institutions.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and / or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

The utility model relates to the technical field of primate animal feeding, in particular to a primate animal movement device which is used in cooperation with a cage body, a primate animal movement space is formed in the cage body, and the cage body is used in cooperation with the primate animal movement device. The movement device of the primate animal comprises a hanging piece and a device main body, and the hanging piece is movably hung in the cage body; the device main body is movably hung below the hanging piece, and the device main body provides an activity space for primates. The device body and the hanging pieces are arranged in the cage body, vines or branches are simulated through the arrangement of the hanging pieces and the device body, so that movement of primates in the cage body is facilitated, the exercise ability, entertainment and playing interests and instincts of the primates in the feeding process are improved, the coordination and flexibility of the primates in physical exercise are improved, and the primates are prevented from being damaged. The method is helpful for the primates to establish social contact with companions and enhance emotion, and also helpful for utilizing resources in the natural environment to meet own demands.

A method for automatically scoring primate actions based on a behavioral interaction network includes the following steps: Step 1, acquiring primate video images, scoring and labeling them; the primate video images include normal behavior, post-medication behavior, and sham surgery behavior; Step 2, extracting monkey features from the primate video images using a neural network; training the neural network three times using the monkey features: the first training input is monkey features for normal behavior, the second training input is monkey features for sham surgery behavior, and the third training input is monkey features for post-medication behavior; finally, outputting the primate action score through a GAP layer and a fully connected layer.

The present invention provides a method for producing oocytes or follicles, or a reconstituted ovary containing oocytes or follicles, the method comprising a step for culturing a reconstituted ovary of a primate under a floating culture condition.

The utility model provides an electric lifting transfer trolley for non-human primates, and relates to the technical field of non-human primate transfer devices, the electric lifting transfer trolley comprises a frame, a transfer cage and a lifting motor, at least one frame layer is arranged in the height direction of the frame, and the transfer cage is arranged on the frame layer through a bracket; wherein the frame is further provided with a threaded rod in the height direction, the bracket is provided with a threaded sleeve, the threaded sleeve is in threaded connection with the threaded rod, and the lifting motor drives the threaded rod to rotate, so that the threaded sleeve does linear motion on the threaded rod, and then the bracket is driven to move up and down. The height of the transfer cage can be adjusted so as to adapt to feeding cages with different heights, and non-human primates can be transferred conveniently.

The present application belongs to the technical field of animal visual function detection, and discloses a non-human primate visual function and behavior detection method. The method comprises: 1. behavior experiment analysis of animals searching for and grabbing food in multiple small squares in an interactive environment with experimenters; and 2. primate climbing motion behavior analysis in a single behavior device cage without disturbance. The method of the present application can accurately capture the visual function deficiency and abnormal behavior of primates for a single time or multiple times in succession, and evaluate the behavior ability and visual function change of primates through digital processing. The method can effectively make up for the problems of conventional ophthalmic detection in evaluating the visual function deficiency and behavior defects of primates, provide more data parameters, deeply analyze the visual function and individual differences of each primate, and accurately reflect the visual function and corresponding behavior change.

The utility model discloses a device for testing the lower limb muscle strength of a non-human primate animal, and the device comprises a device body which comprises an animal fixing box, the back of the animal fixing box is provided with an animal access door, and the top of the animal fixing box is provided with an animal head fixing device; through holes allowing the arms of the animals to extend out are symmetrically formed in the two sides of the animal fixing box, a muscle strength testing device and a fixing ring used for being fixed to the ankles of the animals are arranged in the animal fixing box, and the muscle strength testing device is connected with the fixing ring and used for detecting lower limb muscle strength data of the non-human primates. The lower limb muscle force data of the non-human primate animal can be accurately recorded, so that the recovery degree of the lower limb muscle force of the non-human primate animal can be scientifically and objectively observed, and objectivity, scientificity and accuracy of animal medical experiment data are facilitated.

The present invention relates to a method for identifying the aging degree of a subject or a tissue or organ thereof, and for evaluating the effect of anti-aging interventions. Specifically, provided is a method for identifying the aging degree of a subject or a cell, tissue or organ thereof, or for evaluating the biological age of the subject or diagnosing premature aging disorders, which method predicts the aging status of a tissue or organ of a subject on the basis of the levels of DNA methylation, gene transcripts, proteins or metabolites. The present invention further relates to the use of metformin in the preparation of a drug for treating, preventing or delaying aging-related diseases or conditions in primates, preferably humans.

The present invention relates to a primate model of central motor function impairment. More specifically, the present invention provides: a primate model of central motor function impairment, in which the use of the hand is lost due to damage to a brain region associated with manual dexterity, from immediately after the damage through the acute and chronic periods, while functions of other regions are preserved; and a method for producing the model.

The present invention relates to a method for identifying new individuals of golden snub-nosed monkeys. The method uses a twin network and a deep feature clustering algorithm to classify golden snub-nosed monkey image data without identity information by individual. The method uses three modules, namely a golden snub-nosed monkey facial image pre-training encoder module, a golden snub-nosed monkey category number estimation module, and a label-free data clustering module. This method solves the problem that some image data with missing identity information and wild primate data without identity information in practice ultimately lead to the inability to perform identity recognition. Further, new individuals of primates with high recognition accuracy are added to the original data set to perform data enhancement and improve the model's recognition ability for new individuals. It can be seen from the experimental results that the error between the estimated number of categories and the actual number of categories is within 3, and SS-NIR has good performance in identifying new individuals of golden snub-nosed monkeys.

The present invention provides a method for targeting a maternal UBE3A allele to establish an animal model of Angelman syndrome and use thereof. By means of extensive and in-depth research and screening of the sequences of primate genome UBE3A genes, a target suitable for genetic engineering is obtained: an intron upstream of and / or an intron downstream of exon 5 of a maternal UBE3A gene. An animal model of Angelman syndrome can be obtained by performing downregulation targeting the target. The animal model has a stable and controllable state and a typical disease phenotype, which is conducive to observation.

The feeding barrel for the non-human primates comprises a barrel body and a barrel cover detachably installed on the barrel body, the barrel body is in a barrel shape with the top open, and the side wall of the barrel body is hollowed out so that the feeding position can be observed conveniently; the barrel cover is detachably hung on the top wall of the rearing cage, and the barrel body and the barrel cover are made of food-grade hard materials. According to the feeding barrel for the non-human primates, food is placed in the feeding barrel, so that the food is effectively prevented from being contaminated by dirt in a rearing cage; the hand and the arm of a non-human primate extend into the feeding barrel from the feeding hole for feeding, and meanwhile, the position of food is observed through the hollow structure, so that foraging is facilitated, the fun of exploring and finding in the foraging process of the non-human primate is increased, and animal welfare is increased; the barrel body and the barrel cover are preferably made of food-grade hard materials, can be effectively prevented from being damaged by non-human animals, are convenient to clean and meet the strict requirements of industries such as medicine research and development on sanitary conditions.

The invention discloses a feeding device suitable for primates, and relates to the technical field of animal feeding devices, the feeding device comprises a shell, a food storage trough, a turntable, a first rotating shaft and an operating device; the turntable is arranged in the shell and is rotationally connected with the inner wall of the shell through a first rotating shaft, and the outer wall surface of the turntable is sunken inwards to form a groove; the food storage trough is provided with a food outlet, and the rotating disc is located below the food outlet. One end of the first rotating shaft penetrates through the side wall of the shell and is connected with an operating device, and the operating device drives the rotating disc to rotate through the first rotating shaft, so that the groove can rotate to the position correspondingly communicated with the food storage trough, and food in the food storage trough falls into the groove; a food taking opening is formed in the lower portion of one side of the shell, and the groove can enable food to fall to the inner bottom of the shell in the rotating process and the food can move to the food taking opening along the inner bottom of the shell. According to the invention, an experimenter can conveniently monitor and analyze exploration activities and food desire of primates.

The invention belongs to the technical field of disease animal model construction, and particularly relates to a construction method and application of a CUMS depression model based on bee behavioristics, and the construction method comprises the following steps: carrying out adaptive culture on bees for 22-26 hours, and then carrying out stress treatment for 8 days; a stress source for stress treatment is oscillation treatment, hunger treatment, isoamyl acetate odor treatment and electric shock treatment; during stress treatment, each stress source is given for two days in a random mode, and the same stimulation cannot be accepted for two continuous days. According to the method, the CUMS depression model is constructed by taking bees as research objects, and compared with zebra fish, the bees as social insects have social structures similar to those of human beings and can simulate some behaviors and phenomena in the human society; compared with primates and rodents, the bees are lower in breeding cost and high in breeding speed, and compared with the primates, the bees are used as experimental animals, and the dispute in ethics is relatively small.

The invention provides a primate-based intelligent noninvasive brain pacemaker performance and effectiveness verification method and system, and the method and system show significant advantages in the aspects of technology, economy and experimental ethics. According to the method, high space-time resolution source imaging combining high-density EEG and magnetic resonance imaging (MR I) is adopted, the system can accurately capture TES-induced neural oscillation changes (the time resolution is 1ms) through a millisecond-level dynamic response correlation technology, and the spatial positioning accuracy smaller than or equal to 2mm is achieved in a Parkinson disease macaque model. In addition, long-term dynamic research as long as 6 months is supported, a reliable basis is provided for clinical transformation, and it is predicted that the clinical test failure rate can be reduced by 20%. The method not only accords with the '3R principle 'of animal experiments, but also provides an innovative platform with scientific value and social benefit for brain disease treatment research through non-invasive, high-precision and multi-dimensional technical breakthrough.

Disclosed are constructs and methods for expressing DNA of interest, in particular in non-primate eukaryotic host cells that exhibit advantages in the amount and quality of expression, including high expression stability, and, if appropriate, transport of expression products from the cells.

The application belongs to the field of animal research, and discloses a non-human primate cooperation experiment method and device, which comprises the following steps: step one, using open source single-chip microcomputer code editing software to write required cooperation logic code and burning the code into a circuit board; step two, starting the animal cooperation experiment device; step three, an animal operating a pressing part; step four, judging the triggering condition of the pressing part, if the condition meets the cooperation logic, the cooperation experiment device outputs food, otherwise, the cooperation experiment device outputs a punishing content; and step five, resetting the animal cooperation experiment device; when multiple non-human primates complete cooperation, the application uses food as a reward to encourage cooperation among animals, so that the cooperation mode among non-human primates can be studied, the food stored in the food bin can be supplemented into the temporary storage cavity after the reward, so that the temporary storage cavity always has food, and the device has a small volume and can be installed outside a monkey cage for use at any time.

The utility model relates to the technical field of feeding of primates, in particular to a manger device for the primates. According to the feeding trough, the feeding trough body is arranged outside the cage body through the frame, and the feeding trough body comprises the containing part and the blocking piece. According to the feeding trough for the primate animal, the primate animal can eat the feed placed in the containing part, in the feeding process, the feed is prevented from falling into the cage body, and due to the fact that the feeding trough body comprises the blocking piece, the blocking piece prevents the feed from being stirred out of the feeding trough body by the primate animal in the feeding process of the primate animal. Moreover, a leakage-proof feed tray is further arranged in the cage body, the leakage-proof feed tray and the feeding trough body are arranged on the inner side and the outer side of the cage body respectively, and the leakage-proof feed tray can extend out of the cage body to be connected with the feeding trough body. During feeding, primates can be fed in the cage body and the leakproof feed tray, so that the feed can be prevented from being scattered in the cage body, and the cleanness in the cage body is ensured.

The invention relates to the technical field of biology, and discloses mRNA (messenger Ribonucleic Acid) for coding an antibody and application thereof. The mRNA for coding the antibody comprises mRNA for coding a light chain and mRNA for coding a heavy chain; wherein the sequences for coding the heavy chains CDR1, CDR2 and CDR3 are respectively the 201 to 221 , the 264 to 311 and the 408 to 434 as shown in SEQ ID NO: 1, and the sequences for coding the light chains CDR1, CDR2 and CDR3 are respectively the 177 to 218 , the 264 to 284 and the 381 to 413 as shown in SEQ ID NO: 5. When the mRNA is delivered into a non-human primate body, the non-human primate body can be effectively protected from novel coronavirus infection.

The invention provides a non-human primate fine motion and posture tracking automation method, which is implemented by taking a monkey as a representative, and comprises the following steps: defining 10 motion and 3 posture labels of the monkey, defining 15 key points of the monkey body, constructing a monkey motion identification data set and a monkey key point data set, and establishing a monkey motion and posture identification analysis tool box. The monkeys are subjected to action recognition and skeleton point recognition, and the three postures of platform, sitting and curling up of the monkeys, the engraving behavior and the depression behavior are automatically recognized. According to the invention, automatic identification of fine actions and postures of the non-human primates can be effectively realized, and particularly, effective automatic identification of engraving behaviors, depression behaviors and the like of the non-human primates can be realized, so that the blank of such researches in the field is filled; the method is of great significance to clinical research of difficult miscellaneous diseases such as depression and Rett syndrome.

The application discloses a hyperuricemia non-human primate model and a construction method thereof, and belongs to the technical field of experimental animals. The construction method comprises the following steps: using a non-human primate, i.e., a cynomolgus monkey, as a model animal, orally administering potassium oxonate and intravenously injecting myo-inositol, so that the blood uric acid content of the cynomolgus monkey is significantly increased within 0.5-8 hours, and the process can be inhibited by a uric acid-lowering drug allopurinol, and based on this, a hyperuricemia model of a non-human primate can be formed. Compared with a model constructed by using rodents, the hyperuricemia non-human primate model constructed by the application can better simulate the related symptoms and physiological processes of human hyperuricemia, and provides a preclinical effect evaluation tool for the research and development of nutrients and drugs in related fields.

The technology described herein generally relates to methods and compositions for the modulated and controlled expression of a transgene in a subject (e.g., a primate subject).

This invention generally relates to the field of somatic gene therapy by using viral vectors, and in particular adeno-associated virus (AAV) vectors for the treatment of inherited or acquired diseases. More specifically, the invention relates to a viral capsid protein that provide for a specific transduction of murine endothelial cells for treating or preventing a heart disease in a primate. The viral capsid protein was found to specifically bind to primate heart tissue cells, and in particular primate heart muscle cells, and can be used to provide for an efficient and selective transduction of primate cardiomyocytes and ensure heart tissue-specific expression of one or more transgenes in the primate. The invention further relates to a recombinant viral vector, preferably an AAV vector, which comprises a capsid with at least one transgene packaged in the capsid. The viral vector is suitable for the therapeutic treatment of a cardiac disorder or disease in a primate. The invention further relates to cells and pharmaceutical compositions which comprise the viral vector according to the invention.