71 results about "ORDER PRIMATES" patented technology

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

The present invention provides compositions and methods of treatment of diseases that are sensitive to drugs that downregulate the function of microRNA's, mRNA, non-coding RNA, or viral genomes. In particular, it has been discovered that a very long term effect of an anti microRNA oligonucleotide may be obtained when administered to a primate. Therefore, the present invention relate to pharmaceutical compositions and methods for treatment of primates, including humans wherein the compositions are administered with a long time interval.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhance a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric structure which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particular a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.

The invention provides an automatic grabbing training and recording device for non-human primates for solving the technical problem how to enable trained primates to rapidly grasp various specified grabbing actions. The device comprises a rotating disc body, a microcontroller, a water feed control system, a brain electrical signal recorder and a PC. The device is easy to operate and high in training efficiency. The training mode is adjustable as needed, and the training paradigm of different grabbing masks can be achieved; the gestures are accurately detected, the specified experimental paradigm is conveniently designed, data precision of follow-up analysis is improved, and later analysis and arrangement are convenient. According to the automatic grabbing training and recording device, specified gesture grabbing training of non-human primates can be achieved, the grabbing training of the non-human primates can be rapidly and efficiently achieved through the painless automatic training process, manpower resource consumption can be greatly reduced, and the automatic grabbing training and recording device is applicable to research and development of rehabilitation therapy instruments in the field of brain-computer interfaces.

The invention discloses a method for preparing an animal pulmonary fibrosis model. According to the method, bleomycin is applied to a primate, the application dose of the bleomycin is 7 mg/Kg for each time, and application is performed once for each week and totally performed twice. The invention further provides a rhesus monkey pulmonary fibrosis model prepared with the method and an application of the rhesus monkey pulmonary fibrosis model. The rhesus monkey pulmonary fibrosis model is established successfully, an operation process is simple and easy to repeat, and the model can be used for new biotechnological drug evaluation which cannot be finished by a rodent model and has bright clinical application prospects.

The invention provides a method for establishment of a Mycobacterium tuberculosis naturally infected non-human primate model. According to the invention, a healthy non-human primate and a non-human primate artificially infected with tuberculosis and suffering cough are raised in an air flow self-circulation combined type negative pressure isolator so as to infect the healthy non-human primate with tuberculosis. Establishment of the natural infection model helps to reveal conditional factors (like HIV) activating latent tuberculosis infection and related mechanisms. The non-human primate model with more clinical significance is provided for research, development and evaluation of anti-tuberculosis vaccines and drugs.

The subject invention comprises a method for identifying an evolutionarily meaningful nucleotide change in a primate's TLR4 polynucleotide. It further comprises methods for identifying agents that interact with the corresponding evolutionarily meaningful amino acid change so as to modulate the function of the TLR4 polypeptide, thereby attenuating activation of the NF-kB pathway. Such agents are useful in mitigating the LPS mediated response and in the treatment of sepsis, severe sepsis and septic shock.

The present invention discloses a method for testing memory, judgment and execution capabilities of a primate. The method comprises the following steps that: a testing device is disposed before a primate, the testing device is connected to a monitoring computer (20), and monitoring software is started; and the monitoring computer (20) automatically records the result tested every time and stores the test results in corresponding storage paths as data files. The present invention also discloses a structure of the testing device. By adoption of the testing device, the primate is liable to understand test schemes, and domestication success rate is high; behaviors such as reaction capability and arm and finger agility of the primate can be monitored conveniently, and automation requirements of data acquisition can be satisfied; and the method has the advantages that accuracy of experimental data is high, reaction speed is fast, and jamming is prevented.

The invention discloses a feeding method capable of increasing survival rate of juvenile primates. According to the feeding method, numerous factors required to be considered are integrated, cages andfeedstuff are improved, mutual cross infection of the juvenile primates under diseased conditions is avoided, and strict check and monitor are carried out in the aspect of quarantine inspection, so that the investment is small, the cost is low, the effect is remarkable, and the method is convenient; and death rate standards of this industry is successfully lowered, resources are saved, and the benefit of enterprises is improved.

The invention discloses a kit and a method for primate neuron separation, and relates to the technical field of biological cells. According to the kit disclosed by the invention, the formulas of the artificial cerebrospinal fluid and the brain tissue repairing fluid are optimized, and the inhibitor mixture is added into the artificial cerebrospinal fluid, so that the survival rate of neurons is increased, the neurotoxicity is reduced, and the kit is more suitable for separating the brain cells of primates. According to the kit, the single neuron of the primate animal is successfully separated for the first time, survival of more than 90% of the neuron can be guaranteed, cell bodies, dendrites, axons, other protrusions and the like of the neuron can be reserved, almost all information of the single neuron of the primate animal can be detected, heterogeneity of different neuron can be deeply known, and more comprehensive and complete information of neurons is explored.

Methods are disclosed for correcting a mutant allele of a gene of interest in a primate cell. The methods include a) introducing a non-naturally occurring targeted nuclease and site-specific nucleotide-binding guide that act together to introduce double-stranded breaks in the mutant allele into the primate cell, wherein: i) the primate cell is undergoing mitotic cell division; ii) the primate cell comprises a genome that is heterozygous for the mutant allele, such that the genome comprises one copy of the mutant allele and one copy of a wild-type allele; iii) single-stranded oligonucleotides homologous to the wild-type allele are not introduced into the primate cell. The methods also include b) allowing the primate cell to activate homology-directed repair of the double-stranded DNA breaks in the mutant allele, thereby correcting the mutant allele using the normal wild-type allele as a repair template and producing a primate cell that is homozygous for the wild-type allele. The primate cell can be a one-cell embryo and/or a human cell.