28 results about "ORDER PRIMATES" patented technology

The utility model discloses a non-contact drinking water system for non-human primates, which comprises a cage, a main line water supply unit and a branch line water supply unit, the main line water supply unit comprises a constant-pressure water source and a drinking water main pipeline, and the drinking water main pipeline is communicated with the interiors of the constant-pressure water source and the branch line water supply unit; one end of the branch water supply unit is communicated with the drinking water main pipeline, and the other end of the branch water supply unit is communicated with the interior of the cage; a water inlet hole is formed in one side of the cage, and the water inlet hole is communicated with the interior of the branch water supply unit. The non-human primate breeding device can well adapt to the breeding scale, funds and breeding technology of the non-human primates, is simple in structure, low in cost, energy-saving and environment-friendly, and can avoid breeding water supply pollution so as to be beneficial to the physical health of the non-human primates.

A tree-dwelling primate identification method based on thermal infrared and visible light image fusion relates to the technical field of target detection, and comprises the following steps: synchronously acquiring a visible light image and a thermal infrared image in a target sample plot; performing image processing on the visible light image and the thermal infrared image; extracting thermal infrared features in the thermal infrared image and biological features in the visible light image; based on the extracted thermal infrared features and biological features, performing a three-layer matching mechanism of rough matching, static matching and biological constraint; carrying out image fusion on the visible light image and the thermal infrared image; carrying out model training and optimization; and outputting a species identification result. According to the method, adaptive infrared radiation correction enhancement is carried out on the complex inhabiting environment of the tree-dwelling primates in the high-altitude area, then thermal infrared features and visible light features are fused to carry out cross-modal feature three-layer matching and modeling, interference of environmental conditions can be effectively eliminated, the recognition precision is improved, and the recognition efficiency is improved. And a key technical support is provided for physiological monitoring of the tree-dwelling primates.

This invention relates to the field of primate husbandry technology, and more particularly to a primate feeder, comprising a box fixedly mounted on a feeding cage, a movable frame slidably mounted inside the box, multiple feed outlets communicating with the interior of the box surface, baffles symmetrically and rotatably mounted at each of the feed outlets, multiple support blocks fixedly mounted on the movable frame, a feed tray slidably mounted between every two support blocks, and a support plate fixedly mounted between every two support blocks, the support plate having a locking component for locking the feed tray. This invention, through the cooperation of the box, movable frame, support components, support blocks, sliding component, locking component, and feed tray, allows feed to be pre-placed in the feed tray, and then the tray to be pushed into the feeding cage, thus achieving non-contact feeding, effectively preventing the feeder from being attacked by the animal during feeding, and significantly improving safety.

Provided is a chemical reprogramming method for obtaining an induced pluripotent stem cell from a somatic cell of a primate. Further provided is a composition containing a chemical inducer that can be used in the method.

This invention relates to the field of molecular marker screening technology, specifically to a SNP combination and method for paternity testing in cynomolgus monkeys. The method provided by this invention utilizes 100 selected specific SNP combinations (SNP1~SNP100) combined with low-depth sequencing for paternity testing in cynomolgus monkeys. The cost per sample is only 1 / 5 to 1 / 10 of existing high-depth sequencing and 1 / 2 of SNP chips. For large-scale experimental monkey populations (such as populations of thousands), this can save hundreds of thousands of yuan in testing costs annually, significantly reducing the economic burden of breeding and management of experimental monkeys, demonstrating extremely high economic efficiency. The method provided by this invention is applicable to cynomolgus monkey population pedigree management, kinship tracing, breeding pair optimization, and genetic quality control, and can be widely applied in experimental monkey breeding farms, primate research centers, and biomedical research institutions.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and / or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

A method for automatically scoring primate actions based on a behavioral interaction network includes the following steps: Step 1, acquiring primate video images, scoring and labeling them; the primate video images include normal behavior, post-medication behavior, and sham surgery behavior; Step 2, extracting monkey features from the primate video images using a neural network; training the neural network three times using the monkey features: the first training input is monkey features for normal behavior, the second training input is monkey features for sham surgery behavior, and the third training input is monkey features for post-medication behavior; finally, outputting the primate action score through a GAP layer and a fully connected layer.

The present application belongs to the technical field of animal visual function detection, and discloses a non-human primate visual function and behavior detection method. The method comprises: 1. behavior experiment analysis of animals searching for and grabbing food in multiple small squares in an interactive environment with experimenters; and 2. primate climbing motion behavior analysis in a single behavior device cage without disturbance. The method of the present application can accurately capture the visual function deficiency and abnormal behavior of primates for a single time or multiple times in succession, and evaluate the behavior ability and visual function change of primates through digital processing. The method can effectively make up for the problems of conventional ophthalmic detection in evaluating the visual function deficiency and behavior defects of primates, provide more data parameters, deeply analyze the visual function and individual differences of each primate, and accurately reflect the visual function and corresponding behavior change.

The utility model discloses a device for testing the lower limb muscle strength of a non-human primate animal, and the device comprises a device body which comprises an animal fixing box, the back of the animal fixing box is provided with an animal access door, and the top of the animal fixing box is provided with an animal head fixing device; through holes allowing the arms of the animals to extend out are symmetrically formed in the two sides of the animal fixing box, a muscle strength testing device and a fixing ring used for being fixed to the ankles of the animals are arranged in the animal fixing box, and the muscle strength testing device is connected with the fixing ring and used for detecting lower limb muscle strength data of the non-human primates. The lower limb muscle force data of the non-human primate animal can be accurately recorded, so that the recovery degree of the lower limb muscle force of the non-human primate animal can be scientifically and objectively observed, and objectivity, scientificity and accuracy of animal medical experiment data are facilitated.

The present invention relates to a method for identifying the aging degree of a subject or a tissue or organ thereof, and for evaluating the effect of anti-aging interventions. Specifically, provided is a method for identifying the aging degree of a subject or a cell, tissue or organ thereof, or for evaluating the biological age of the subject or diagnosing premature aging disorders, which method predicts the aging status of a tissue or organ of a subject on the basis of the levels of DNA methylation, gene transcripts, proteins or metabolites. The present invention further relates to the use of metformin in the preparation of a drug for treating, preventing or delaying aging-related diseases or conditions in primates, preferably humans.

The present invention relates to a primate model of central motor function impairment. More specifically, the present invention provides: a primate model of central motor function impairment, in which the use of the hand is lost due to damage to a brain region associated with manual dexterity, from immediately after the damage through the acute and chronic periods, while functions of other regions are preserved; and a method for producing the model.

The present invention provides a method for targeting a maternal UBE3A allele to establish an animal model of Angelman syndrome and use thereof. By means of extensive and in-depth research and screening of the sequences of primate genome UBE3A genes, a target suitable for genetic engineering is obtained: an intron upstream of and / or an intron downstream of exon 5 of a maternal UBE3A gene. An animal model of Angelman syndrome can be obtained by performing downregulation targeting the target. The animal model has a stable and controllable state and a typical disease phenotype, which is conducive to observation.

The feeding barrel for the non-human primates comprises a barrel body and a barrel cover detachably installed on the barrel body, the barrel body is in a barrel shape with the top open, and the side wall of the barrel body is hollowed out so that the feeding position can be observed conveniently; the barrel cover is detachably hung on the top wall of the rearing cage, and the barrel body and the barrel cover are made of food-grade hard materials. According to the feeding barrel for the non-human primates, food is placed in the feeding barrel, so that the food is effectively prevented from being contaminated by dirt in a rearing cage; the hand and the arm of a non-human primate extend into the feeding barrel from the feeding hole for feeding, and meanwhile, the position of food is observed through the hollow structure, so that foraging is facilitated, the fun of exploring and finding in the foraging process of the non-human primate is increased, and animal welfare is increased; the barrel body and the barrel cover are preferably made of food-grade hard materials, can be effectively prevented from being damaged by non-human animals, are convenient to clean and meet the strict requirements of industries such as medicine research and development on sanitary conditions.

The invention belongs to the technical field of disease animal model construction, and particularly relates to a construction method and application of a CUMS depression model based on bee behavioristics, and the construction method comprises the following steps: carrying out adaptive culture on bees for 22-26 hours, and then carrying out stress treatment for 8 days; a stress source for stress treatment is oscillation treatment, hunger treatment, isoamyl acetate odor treatment and electric shock treatment; during stress treatment, each stress source is given for two days in a random mode, and the same stimulation cannot be accepted for two continuous days. According to the method, the CUMS depression model is constructed by taking bees as research objects, and compared with zebra fish, the bees as social insects have social structures similar to those of human beings and can simulate some behaviors and phenomena in the human society; compared with primates and rodents, the bees are lower in breeding cost and high in breeding speed, and compared with the primates, the bees are used as experimental animals, and the dispute in ethics is relatively small.

Disclosed are constructs and methods for expressing DNA of interest, in particular in non-primate eukaryotic host cells that exhibit advantages in the amount and quality of expression, including high expression stability, and, if appropriate, transport of expression products from the cells.

The application belongs to the field of animal research, and discloses a non-human primate cooperation experiment method and device, which comprises the following steps: step one, using open source single-chip microcomputer code editing software to write required cooperation logic code and burning the code into a circuit board; step two, starting the animal cooperation experiment device; step three, an animal operating a pressing part; step four, judging the triggering condition of the pressing part, if the condition meets the cooperation logic, the cooperation experiment device outputs food, otherwise, the cooperation experiment device outputs a punishing content; and step five, resetting the animal cooperation experiment device; when multiple non-human primates complete cooperation, the application uses food as a reward to encourage cooperation among animals, so that the cooperation mode among non-human primates can be studied, the food stored in the food bin can be supplemented into the temporary storage cavity after the reward, so that the temporary storage cavity always has food, and the device has a small volume and can be installed outside a monkey cage for use at any time.

The application discloses a hyperuricemia non-human primate model and a construction method thereof, and belongs to the technical field of experimental animals. The construction method comprises the following steps: using a non-human primate, i.e., a cynomolgus monkey, as a model animal, orally administering potassium oxonate and intravenously injecting myo-inositol, so that the blood uric acid content of the cynomolgus monkey is significantly increased within 0.5-8 hours, and the process can be inhibited by a uric acid-lowering drug allopurinol, and based on this, a hyperuricemia model of a non-human primate can be formed. Compared with a model constructed by using rodents, the hyperuricemia non-human primate model constructed by the application can better simulate the related symptoms and physiological processes of human hyperuricemia, and provides a preclinical effect evaluation tool for the research and development of nutrients and drugs in related fields.

The technology described herein generally relates to methods and compositions for the modulated and controlled expression of a transgene in a subject (e.g., a primate subject).

This invention generally relates to the field of somatic gene therapy by using viral vectors, and in particular adeno-associated virus (AAV) vectors for the treatment of inherited or acquired diseases. More specifically, the invention relates to a viral capsid protein that provide for a specific transduction of murine endothelial cells for treating or preventing a heart disease in a primate. The viral capsid protein was found to specifically bind to primate heart tissue cells, and in particular primate heart muscle cells, and can be used to provide for an efficient and selective transduction of primate cardiomyocytes and ensure heart tissue-specific expression of one or more transgenes in the primate. The invention further relates to a recombinant viral vector, preferably an AAV vector, which comprises a capsid with at least one transgene packaged in the capsid. The viral vector is suitable for the therapeutic treatment of a cardiac disorder or disease in a primate. The invention further relates to cells and pharmaceutical compositions which comprise the viral vector according to the invention.

The present application relates to the field of immune cell therapy, and provides a chimeric antigen receptor based on the modification of the receptor tyrosine kinase family AXL and its application. The chimeric antigen receptor contains an extracellular binding domain, and the extracellular binding domain can specifically bind to the AXL ligand; the amino acid sequence of the AXL is shown in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5. Target specificity: GAS6 is lowly expressed on the cell membrane of normal cells, but highly expressed on the cell membrane of tumor tissues, so that the CAR provided by the present invention can specifically kill tumor cells with high expression of GAS6 on the membrane without obvious toxic effects on normal cells. The present invention utilizes the mode of action of ligand binding to receptor, rather than scFv in the traditional sense. The conservation of receptor-ligand interaction determines the safety test in animals, especially primates, which can better reflect its safety in humans.

The invention provides a method for establishing an angel syndrome animal model by targeting a UBE3A maternal allele and application. On the basis of extensive and in-depth research and screening of sequences of primate genome UBE3A genes, a target suitable for gene modification is obtained: an upstream intron and / or a downstream intron of maternal UBE3A gene exon5, and an angel syndrome animal model can be obtained by targeting the target to implement down regulation. The animal model is stable and controllable in state, typical in disease phenotype and easy to observe.

The present disclosure relates to compositions and methods of treating Alzheimer's disease, and generating new neurons in a primate using an adeno-associated viral (AAV) vector encoding NeuroD1.

The utility model relates to a non-human primate experiment restraint device, and relates to the technical field of animal restraint devices.The non-human primate experiment restraint device comprises a base, a backrest assembly and a head assembly, the base comprises a bottom frame and a pair of supporting columns, and the supporting columns are vertically arranged and distributed on the two sides of the top of the bottom frame; the backrest assembly comprises a backup plate and a seat plate, the backup plate is vertically arranged and hinged to the pair of supporting columns through damping shafts, the seat plate is horizontally arranged below the backup plate, and a first binding assembly used for binding the waist of the primate is arranged on the backup plate; the head assembly comprises a head plate and a telescopic assembly, the head plate is horizontally arranged above the backup plate, a head hole allowing the head of the primate to penetrate through is formed in the head plate, and the telescopic assembly is horizontally and slidably connected with the head plate and used for adjusting the size of the head hole. By arranging the backrest assembly capable of adjusting the rotation angle, adjustment of the sitting posture of the primate is achieved, it is guaranteed that the primate is in a comfortable state during an experiment, and then the accuracy of an experiment result is guaranteed.

The present invention relates to a method for producing a nonhuman primate animal model of cerebral infarction, comprising administering endothelin to basal ganglia and thalamic region of a nonhuman primate, and thereby inducing basal ganglia damage, thalamus damage, and internal capsule damage; and a pharmaceutical composition for the treatment of cerebral infarction at a subacute to chronic stage, penetrating branch infarction, or cerebral infarction having brain damage in a penetrating branch territory, comprising a NeuroD1 protein or a polynucleotide encoding the NeuroD1 protein.

To provide methods and lipid nanoparticles for delivery of a therapeutic agent to primates for treatment of various indications.SOLUTION: A method for delivering a nucleic acid to a primate in need of treatment is provided which comprises administering a lipid nanoparticle (LNP) to the primate, the LNP comprising: i) a nucleic acid, or a pharmaceutically acceptable salt thereof, encapsulated in the LNP; ii) a cationic lipid; iii) a neutral lipid; iv) a steroid; and v) a polymer-conjugated lipid, where a plurality of the LNPs has a mean particle diameter ranging from 40 nm to 70 nm.SELECTED DRAWING: Figure 13

The application provides a non-human primate non-anesthesia in-cage body weight measurement system, comprising a movable weighing unit, a cage unit and a movable multifunctional load unit. The position of the weighing unit body of the application is selected above the cage, which is different from the traditional platform weighing from below, reducing the problem that liquid and dirt in the cage flushing process easily cause damage to the weighing unit body. This design also solves the problem that animals need to be transferred to a dedicated weighing cage for weighing, and realizes that animals can complete weighing in their own daily life cage. At the same time, the weighing process of the application has a high degree of automation, the weighing time of a single animal is reduced, the work efficiency of laboratory personnel is improved, the animal anesthesia process is avoided, and the demand for measuring the body weight of adult large-sized non-human primates in a relatively harmonious form under a conscious state is met.

The application discloses a craniotomy device for collecting behavior data of non-human primates, which comprises a craniotomy device body and a master control center, wherein the master control center is arranged outside the craniotomy device body and is used for controlling the action of the craniotomy device body; the craniotomy device body comprises a base and a craniotomy mechanism; the base is annular, a craniotomy positioning hole is formed in the center of the base, the base comprises two layers of shelf plates arranged oppositely and a measuring layer arranged between the two layers of shelf plates, a plurality of distance measuring sensors are arranged in the measuring layer at intervals with a preset distance, the detection heads of the distance measuring sensors are arranged towards the craniotomy positioning hole, and a guide rail is arranged on the shelf plate layer at the top end of the base along the craniotomy positioning hole; and the craniotomy mechanism is slidably arranged on the guide rail. The craniotomy device can grasp the cutting depth and effectively avoid damaging the brain tissue during cutting.

The present invention relates to a primate model of central motor function impairment. More specifically, the present invention provides: a primate model of central motor function impairment, in which the use of the hand is lost due to damage to a brain region associated with manual dexterity, from immediately after the damage through the acute and chronic periods, while functions of other regions are preserved; and a method for producing the model.