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Method for preparing diabetic animal model

A technology of animal model and diabetes, which is applied in the field of preparation of animal models of diabetes, can solve the problem that there is no animal model of type I diabetes, and achieve the effect of clear physiological background

Inactive Publication Date: 2010-02-03
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the use of low-dose STZ for the preparation of animal models of type 1 diabetes

Method used

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  • Method for preparing diabetic animal model
  • Method for preparing diabetic animal model
  • Method for preparing diabetic animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of the animal model of the present invention

[0022] 1. Materials and methods:

[0023] 1.1 Experimental materials

[0024] Two female and two male rhesus monkeys, 2-3 years old, free of herpes virus B, monkey retrovirus, monkey leukemia virus and immunodeficiency virus infection; purchased from Chengdu Pingan Animal Breeding Base.

[0025] 1.2 Main reagents

[0026] Surgical drugs and consumables were purchased from Likang Pharmacy of West China Hospital

[0027] 1.3 Preparation of the main solution

[0028] Preparation of STZ: Weigh 100 mg of streptozotocin (STZ), add 5 ml of 0.05 mol / L citric acid buffer (pH = 4.5) to dissolve (20 mg / ml). The prepared solution should be injected within 30 minutes.

[0029] 1.4 Main instruments

[0030] 1) 64-channel spiral CT machine: Philips

[0031] 2) Anesthesia machine: Excel 210SE, Datex-Ohmeda, USA

[0032] 3) Ventilator with ECG monitor: M / 206B, Philips

[0033] 4) H.F.ELECTROTOME: Model GD-350-P, Shanghai Hutong Electr...

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Abstract

The invention relates to a method for preparing an animal model of an insulin-dependent diabetes. In the method, the pancreas part of a primate is excised and a method of streptozotocin induction is used together to establish a rhesus monkey diabetes model, wherein the using method and the dosage of streptozotocin are as follows: the streptozotocin is injected in vein in a ratio of 15 to 20 mg / kgeach time. The method establishes the rhesus monkey diabetes model by combining the excision of the pancreas with the method of streptozotocin (STZ) induction, and carries out observation and comparison of physical indexes of the model. The insulin treatment scheme is economical, safe and feasible.

Description

Technical field [0001] The invention relates to a method for preparing an animal model of diabetes. Background technique [0002] The age of onset of type I diabetes is young and the cause is unknown. So far there is no effective preventive method. Although the commonly used insulin therapy can regulate blood sugar levels to a certain extent, long-term use cannot effectively control the occurrence of diabetic complications, and some patients may also experience hypoglycemic shock. The only effective treatment for these patients in the late stage of the disease is to perform pancreas or islet transplantation to restore the body's metabolic function from the physiological level. At present, the shortage of donor organs is a key issue for pancreatic islet transplantation. It is urgent to find new sources of pancreas and islets for human transplantation to meet the treatment needs of diabetic patients. The induced differentiation and large-scale expansion of islet stem cells may be...

Claims

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Application Information

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IPC IPC(8): A61B19/00A61K49/00A61B17/00A61D1/00A61D7/00A61K31/7008
Inventor 陆燕蓉程惊秋麦刚田伯乐陈又南张文胜任艳王莉李宏霞
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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