Diabetes primate model construction method
A primate and construction method technology, which is applied in the field of diabetic primate model construction, can solve the problems of high drug dosage and injection method, shortened diabetes model cycle, and large difference in modeling success rate, etc., to achieve Good persistence and stability, avoiding complications or death, and fast modeling time
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Embodiment 1
[0029] 1. Animal selection
[0030] 6 common-grade cynomolgus monkeys, middle-aged and elderly (about 10-20 years old), weighing more than 8kg, male cynomolgus monkeys, with normal appearance. Animal information is shown in Table 1 below:
[0031] Table 1. Monkey information
[0032]
[0033] 2. Breeding environment
[0034] Each monkey was reared in a single cage, with normal sunshine at the beginning of the experiment, and light at night during the sleep time of the animals in the later period, drinking water normally, and each monkey was fed with high-sugar and high-fat feed (total 150g) with two meals a day.
[0035] 3. Modeling method
[0036] Modeling was induced by increasing doses of streptozotocin (STZ) + high-sugar and high-fat feeding + late-night light combination. For the modeling process, see figure 1 .
[0037] (1) STZ preparation
[0038] STZ was purchased from Sigma Corporation (Lot #WXBC8740V).
[0039] Quickly weigh the amount of STZ powder for eac...
Embodiment 2
[0102] According to the method of Example 1, 50 cynomolgus monkeys were modeled. There were 38 monkeys whose fasting blood sugar was stable above 6.9mmol / L, of which 34 monkeys had HbAlc>6.5%. Glucose tolerance was tested for these monkeys that reached the standard, and they were administered orally. After 2 hours of intragastric administration of glucose, the blood sugar was still above 11.1mmol / L, reaching the standard of diabetes. The success rate of modeling was 68%, and the mortality rate was 0%. See Table 7 below for details.
[0103] Table 7. Indicators of 50 monkeys after modeling
[0104]
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