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A primate and construction method technology, which is applied in the field of diabetic primate model construction, can solve the problems of high drug dosage and injection method, shortened diabetes model cycle, and large difference in modeling success rate, etc., to achieve Good persistence and stability, avoiding complications or death, and fast modeling time
Active Publication Date: 2021-03-02
广东蓝岛生物技术有限公司
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There are the following disadvantages in the preparation of diabetes models by the above induction methods: 1. The period of establishing a diabetes model through high-sugar and high-fat diet induction is longer than 2 years, and the modeling rate is low, less than 5%; 2. The establishment of a diabetes model by light induction The cycle is shortened, but the modeling rate is quite different, and there is a phenomenon of model animal death; 3. The establishment of a diabetes model by injecting streptozotocin requires high dosage and injection method, otherwise it will easily lead to the death of model animals or difficulty in forming a model Or it turns cloudy soon after molding, and the success rate of molding varies greatly
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Embodiment 1
[0029] 1. Animal selection
[0030] 6 common-grade cynomolgus monkeys, middle-aged and elderly (about 10-20 years old), weighing more than 8kg, male cynomolgus monkeys, with normal appearance. Animal information is shown in Table 1 below:
[0031] Table 1. Monkey information
[0032]
[0033] 2. Breeding environment
[0034] Each monkey was reared in a single cage, with normal sunshine at the beginning of the experiment, and light at night during the sleep time of the animals in the later period, drinking water normally, and each monkey was fed with high-sugar and high-fat feed (total 150g) with two meals a day.
[0035] 3. Modeling method
[0036] Modeling was induced by increasing doses of streptozotocin (STZ) + high-sugar and high-fat feeding + late-night light combination. For the modeling process, see figure 1 .
[0037] (1) STZ preparation
[0038] STZ was purchased from Sigma Corporation (Lot #WXBC8740V).
[0039] Quickly weigh the amount of STZ powder for eac...
Embodiment 2
[0102] According to the method of Example 1, 50 cynomolgus monkeys were modeled. There were 38 monkeys whose fasting blood sugar was stable above 6.9mmol / L, of which 34 monkeys had HbAlc>6.5%. Glucose tolerance was tested for these monkeys that reached the standard, and they were administered orally. After 2 hours of intragastric administration of glucose, the blood sugar was still above 11.1mmol / L, reaching the standard of diabetes. The success rate of modeling was 68%, and the mortality rate was 0%. See Table 7 below for details.
[0103] Table 7. Indicators of 50 monkeys after modeling
[0104]
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Abstract
The invention provides a diabetes primate model construction method. The method comprises the following steps of injecting streptozotocin into cynomolgus monkeys according to a small-dose progressiveincrease method, feeding the cynomolgus monkeys with high-sugar and high-fat feed at the same time, and after injecting the streptozotocin into the cynomolgus monkeys and feeding the cynomolgus monkeys with the high-sugar and high-fat feed for a period of time, increasing night illumination to induce the cynomolgus monkeys to become diabetes models. The diabetic monkey model is successfully established by adopting the method for 1.5-6 months, the model has the core characteristics of insulin resistance and hyperglycemia, the duration of hyperglycemia is long, and the hyperglycemia is not reduced after more than 200 days. The animal model established by the invention can be used in animal experiments with longer duration, and can be sold as a mature animal model to generate economic benefits.
Description
technical field [0001] The invention belongs to the technical field of diabetic animal models, and in particular relates to a method for constructing a diabetic primate model. Background technique [0002] Diabetes mellitus is a common disease. Due to insulin deficiency or / and insulin action (resistance), it leads to increased blood sugar and other metabolic disorders. Genetic factors and environmental factors each account for 50% of the pathogenic factors. Appropriate animal models are needed to study the pathogenesis and potential treatments of diabetes. At present, rodent models (big and mice) are mostly used in diabetes research. Modeling methods include spontaneous and chemical induction methods. Therefore, the data obtained from this type of animal model are not suitable for the study of the mechanism of human diabetes and its complications. [0003] Non-human primates (monkeys) have great genetic homology with humans, and their morphological structure, physiological...
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