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A method for constructing a diabetic primate model

A primate and construction method technology, which is applied in the field of diabetic primate model construction, can solve the problems of high drug dosage and injection method, shortened diabetes model cycle, and large difference in modeling success rate, etc., to achieve Good persistence and stability, avoiding complications or death, and fast modeling time

Active Publication Date: 2022-07-19
广东蓝岛生物技术有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are the following disadvantages in the preparation of diabetes models by the above induction methods: 1. The period of establishing a diabetes model through high-sugar and high-fat diet induction is longer than 2 years, and the modeling rate is low, less than 5%; 2. The establishment of a diabetes model by light induction The cycle is shortened, but the modeling rate is quite different, and there is a phenomenon of model animal death; 3. The establishment of a diabetes model by injecting streptozotocin requires high dosage and injection method, otherwise it will easily lead to the death of model animals or difficulty in forming a model Or it turns cloudy soon after molding, and the success rate of molding varies greatly

Method used

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  • A method for constructing a diabetic primate model
  • A method for constructing a diabetic primate model
  • A method for constructing a diabetic primate model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Animal selection

[0030] There are 6 ordinary cynomolgus monkeys, middle-aged and elderly (about 10-20 years old), weighing more than 8kg, male cynomolgus monkeys, with normal appearance. Animal information is shown in Table 1 below:

[0031] Table 1. Monkey Information

[0032]

[0033] 2. Feeding environment

[0034] Each monkey was housed in a single cage, with normal sunlight in the early stage of the experiment, and during the later stage of the experiment, the animals were illuminated during sleep time, and they drank normally.

[0035] 3. Modeling method

[0036] The model was induced by a combination of increasing doses of streptozotocin (STZ) + high-sugar and high-fat feeding + late night light. See the modeling process. figure 1 .

[0037] (1) STZ preparation

[0038] STZ was purchased from Sigma (Lot#WXBC8740V).

[0039] The amount of STZ powder for each monkey was quickly weighed and dissolved in 5 mL of citric acid buffer (pH 4.2-4.5). It is ...

Embodiment 2

[0102] The modeling of 50 cynomolgus monkeys was carried out according to the method of Example 1. The fasting blood glucose of 38 monkeys was stable above 6.9mmol / L, and the HbAlc of 34 monkeys was >6.5%. After 2 hours of intragastric administration of glucose, the blood sugar was still above 11.1 mmol / L, reaching the standard of diabetes. The modeling success rate was 68%, and the mortality rate was 0%, as shown in Table 7 below.

[0103] Table 7. Indicators of 50 monkeys after modeling

[0104]

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Abstract

The invention provides a method for constructing a diabetic primate model. The method is to inject streptozotocin into cynomolgus monkeys according to the method of small dose increasing, and feed high-sugar and high-fat feed at the same time. , increasing nocturnal light to induce cynomolgus monkeys to become a model of diabetes. Using this method, a diabetic monkey model was successfully established for 1.5 to 6 months. The model has two core characteristics of diabetes, insulin resistance and hyperglycemia, and the hyperglycemia lasts for a long time, and has not decreased for more than 200 days. The animal model established by the present invention can be used in animal experiments with a longer duration, and can be sold as a mature animal model to generate economic benefits.

Description

technical field [0001] The invention belongs to the technical field of diabetic animal models, in particular to a method for constructing a diabetic primate model. Background technique [0002] Diabetes mellitus is a common disease that leads to hyperglycemia and other metabolic disorders due to insulin deficiency or / and insulin action (resistance), and genetic factors and environmental factors each account for 50% of its pathogenic factors. Appropriate animal models are needed to study the pathogenesis of diabetes and potential treatments. At present, rodent models (big and mouse) are mostly used in diabetes research. The modeling methods include spontaneous and chemical induction. However, rodents and humans are far from each other. Therefore, the data obtained from this type of animal model are not suitable for the study of the mechanism of human diabetes and its complications. [0003] Non-human primates (monkeys) have great genetic homology with humans, and their morp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A23K10/30A23K10/37A23K20/24A23K20/26A23K20/174A23K20/20A23K20/142A23K20/105A23K20/163A23K20/158A23K10/22A23K50/00A61K49/00A61K31/7008
CPCA01K67/02A61K31/7008A61K49/0008A01K2267/03
Inventor 叶箭梦邱春梅李欣欣李岳锋梁展鹏吴玲环李广业
Owner 广东蓝岛生物技术有限公司
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