32 results about "Polarized light microscopy" patented technology

A microscope system for determining optical properties of a specimen includes a source of polarized light, a detector for detecting the intensity of light incident thereon, an optical path extending from the source to the detector, a condenser for providing light from the source to the specimen, an objective for receiving light from the specimen, a support for mounting the specimen, a sectored variable retarder mounted in the optical path, and a polarized light analyzer mounted in the path between the sectored variable retarder and the detector. The variable retarder has a multiple sectors. Each sector is individually addressable by a control signal that affects the light retardation characteristics of the sector.

There is disclosed a device or apparatus that is particularly suitable for use in the analytical fields of polarized light microscopy and spectroscopy/spectral imaging. The apparatus comprises an automated polarized light microscope combined with means for achieving spectroscopic analysis. The apparatus may optionally include means for spectral imaging associated with the means for achieving spectroscopic analysis. Any means for achieving spectroscopic analysis may be utilized for example Raman, mid-infrared, near-infrared, ultraviolet, visible, luminescence, and the like.

The invention discloses a visual observing system for observing growth design of a microcrystalline silicon film in real time and a measurement method, which effectively improve the defect that a traditional polarization microscope is relatively low in resolution in an observing process. The visual observing system for the growth process of the microcrystalline silicon film comprises a light source, a pinhole, a polarizer, a 1/4 wave plate, a convergent lens, a beam splitting prism, a vertical objective lens, a sample, an objective table, a polarization analyzer, a CCD camera and a computer. The visual observing system has the beneficial effects that a scanning problem in a traditional film thickness detection method is effectively improved, the continuous dynamic detection of the film is realized, the detection efficiency and precision are improved, and the resolution is high; besides, the observing system disclosed by the invention is simple in structure, and the method is simple, convenient and feasible and is convenient to popularize.

The present invention relates to a method for measuring the yarn component basing on the micropolariscope, and the method adopts the micropolariscope for detecting the fiber component of pure and blended yarn and each component ratio. The yarn sample with short segment is scattered to discrete fiber, the discrete fiber is leaded to be arranged trimly in a certain direction and is prepared to the sample piece which can be observed in the micropolariscope, the clear image of the sample piece under the micropolariscope is obtained, and the computer processes the image and outputs the fiber component style and blending ratio. The invention can accurate identify the style of the fiber comprising the yarn; can accurately detect the content proportion of each fiber component of the blending yarn; can totally realize the no-pollution testing and conforms to the basic national policy of energy saving and discharging reducing; the high-pressure electrostatic technique and computer image processing technique are adopted for realizing the automatic stock layout and automatic numbering to the fiber thereby realizing the yarn detecting with high precision and high efficiency, has low testing cost and facilitates the generalizing and applying.

The invention discloses a method for cell imaging by adopting a polarized light microscope to observe nano particles; a single gold nanorod is observed by adopting the polarized light microscope; the polarized light microscope is found to be capable of selective imaging of an anisotropic nano material; under the polarized light microscope, scattering light spots of the gold nanorod has smaller size, the background value of imaging can be significantly reduced, and accordingly, the signal-to-noise ratio of an image is improved by at least 3 times or more. Due to the unique optical properties, the anisotropic nano material and the polarized light microscope have great application potentiality in imaging and detection of cancer cells.

Apparatus for generating polychromatic polarized light with the polarization ellipse orientation determined by the wavelength. The proposed polychromatic polarization state generator can be used in various configurations of polarized light microscope (called “polychromatic polscope”) for imaging birefringent samples. The polychromatic polscope produces a spectral-modulated visual scene, in which birefringent structures are evident because their appearance is different from the background. New polarized light microscope can subtract the background and produce video-enhanced color image of birefringent structures. The obtained picture can be also mathematically processed in order to obtain a map of quantitative distribution of specimen retardation and orientation of the principal axes.

This invention relates to analogue blood bed display iron magnetic substance heating method, which comprises the following steps: quickly cooling it down and cracking it into parts; re-piecing the cracked blood cover slices on the glass slides with gaps between; dripping melted poly glycol between blood cover slice and glass slice; deleting the poly glycol in gaps after the poly glycol solidified; filling the dried iron magnetic power to the above gaps to get the analogue blood bed; putting the above analogue blood bed in the alternating magnetic field and then putting the analogue blood bed under bias microscopes for observation.

The invention provides a dyeing method for observing collagenous fibers by adopting a polarization microscope. The method comprises the following steps: firstly, using saturated picric acid-sirius red dye liquor for dyeing, then using a weak acid solution in concentration of 1-10%w/v for immersing tissue slices, and finally conventionally leaching, drying and fixing the tissue slices. The permeation of the tissue is enhanced by the heated picric acid, so that the lining dyeing background is brighter; the heated sirius red dye liquor has firmer adsorption for the collagenous fibers and the coloring is clear; the weak acid solution can wash off the picric acid crystal separated out of the tissue slices and is difficult to wash off the yellow lining dyeing background, so that the excellent coloring of the collagenous fibers can be guaranteed, the yellow lining dyeing background is obvious, and the collagenous fibers are clear; the dyeing method provided by the invention has high repeatability, the collagenous fibers are obviously colored, and the fiber structure is clear; and the method is beneficial to the positioning and quantitative analysis for different collagenous fibers and is convenient for the judgment for the degree of tissue fibrosis.

The invention relates to a visual detection method for observing lipid adsorption of montmorillonite based on a polarization microscope. Firstly the montmorillonite and high fat food or feed are mixed uniformly; then a sample is prepared into a smear piece; the smear piece is dyed through a neutral lipid dye oil red 0; the prepared smear piece is placed under the polarization microscope for detection. The adsorption property of the montmorillonite to lipid in the high fat food is observed. The method is a physical detection method, can be used for directly observing lipid adsorption of the montmorillonite, and is simple in operation and intuitive and convenient in observing.

The invention relates to the field of polarized light detectionand particularly relates to a method for identifying mineral drugs based on a polarized light microscopy technology. The method comprisesthe following steps of (1) putting mineral medicine with the thickness of 1-2mm on a coarse grinding stone, adding a proper amount of water, and grinding the mineral medicine back and forth on the coarse grinding stone until the two sides are ground to be smooth and the thickness is 0.2-0.9 mm; (2) transferring the mineral medicine ground in the step (1) to a fine grinding stone, adding the water, grinding the mineral medicine back and forth till the mineral medicine is transparent, washing the ground mineral medicine with water, treating the ground mineral medicine with ethanol, and fillingthe ground mineral medicine with a glyceryl ethanol test solution; and (3) putting the abrasive disc prepared in the step (2) under a polarized light microscope with a compensator, setting the angle of semi-polarized light, and shooting under a 10-time objective lens by adopting a large-image splicing technology in combination with a real-time depth-of-field extended imaging technology to obtain color image data. According to the method, the birefringent mineral structure can be accurately positioned, and the multi-color property of anisotropy of structures of different parts of the mineral under polarized light is fully displayed.

The invention discloses a device for shooting a large-area sample on a microscope and a use method thereof. Two electric translation platforms which are orthogonally stacked and mounted together are arranged on a base of the microscope, and a sliding block of the electric translation platform located at the upper part is used as a sample placement platform; an image acquisition sensor is arrangedat the top of the microscope and used for shooting the sample and acquiring an image of the sample; the image acquisition sensor of the microscope is connected with an upper computer, the upper computer controls the image acquisition sensor to shoot, and the image acquired by the image acquisition sensor through shooting is transmitted back to the upper computer for splicing processing of subsequent images; the electric translation platforms are connected with the upper computer, and the upper computer controls the two electric translation platforms to move so as to control the shooting position of the sample. The imaging advantage of the optical polarized light microscope can be fully utilized, compared with a digital camera picture, the shooting resolution is increased by hundreds of times, and a breakthrough is brought to the imaging range of an existing microscope.

The invention discloses a polarizer for a polarizing microscope used for mineral identification, which comprises a base bottom plate, a base support lower polarizer assembly and an upper polarizer assembly, and the base support is vertically arranged on one side of the base base plate , the polarizing microscope is installed on the base plate through a fixing device, the lower polarizing assembly is installed at the middle and lower part of the base support, and the upper polarizing assembly is installed at the upper part of the base support. The automatic polarizer of the polarizing microscope of the present invention realizes the automatic operation of the orthogonal polarized light microscope inspection and the single polarized light microscope inspection through the cooperation of the upper polarizer component and the lower polarizer component, and can perform precise measurement at the same time to increase the accuracy of the microscope inspection.

The invention relates to a polarized light microscopy-X-ray diffraction method for qualitative detection of asbestos in electronic products. The method combines X-ray diffraction and polarized light microscopy observation. By means of observation of a polarized light microscopy, the optical properties of a sample in oil with different refractive indexes are considered, and the asbestos type is preliminarily identified; compared with the separately used X-ray diffraction method, the polarized light microscopy-X-ray diffraction method enables diffraction observation to be targeted, and is accurate in results and good in reproducibility. The method can be used for accurately identifying the trace amount of asbestos contained in a to-be-tested sample of an electronic product, is suitable for the inspection of the asbestos in the various electronic products, and can effectively avoid asbestos hazard and trade risks of the electronic products.

The invention provides an Al-free medical zinc-based alloy dendrite corrosion reagent, and a preparation method and a use method thereof, and relates to the technical field of metallographic analysis of as-cast zinc alloys. The Al-free medical zinc alloy dendrite corrosion reagent is prepared from 3.5-4.5 g of citric acid, 2-3 ml of glacial acetic acid, 4.4-5 g of picric acid, 40-45 ml of ethanol and 50-60 ml of deionized water. Before corrosion, a sample is ground by 1000 #, 2000 #, 3000 # and 5000 # abrasive paper in sequence, and then chemical polishing is carried out by using a nitric acid methanol solution with a specific proportion. The corrosion reagent is dropped on the surface of a zinc alloy in an inclined mode, and when the corrosion agent on the surface of the alloy is turned into reddish brown from light yellow, the zinc alloy is immediately immersed in an ethanol/hydrochloric acid mixed solution for ultrasonic cleaning and air-dried by cold air for later use. A polarized light microscope is adopted to inspect the refined dendritic structure of the alloy. The Al-free medical zinc alloy dendrite corrosion reagent has the advantages of good stability and simple formula, can obtain high-quality contrast in the specific use process, and can finish effective distinguishing of refined dendritic structures and microstructures.

The invention discloses a fiber comparison sheet for court science polarized light microscope inspection and a preparation method thereof. The preparation method comprises the following steps: S1, bonding a plurality of fibers of known materials on a glass slide to form a net shape; S2, covering a cover glass on the glass slide, drying, and marking a fiber type at one end of the glass slide; wherein the length of the fiber is 1-2cm; separating the fibers from a textile or fiber sample of known material using scissors, tweezers, and separation needles. The polarized light inspection of the fibers is mainly used for identifying and distinguishing the types by observing the interference characteristics of different fibers, and the requirement on practical experience is very high. The conditions that characteristics are difficult to confirm and the like often exist only through inspection of a single inspection material or sample, and different types of fibers and fibers of the same type, different manufacturers and different processes can be quickly, effectively and accurately identified and distinguished by comparing the fiber comparison sheet with a known result with the inspection material or sample.

The invention relates to the field of polarized light detection, in particular to a microscopic shooting method for the joint development of a single-refraction plant tissue and a double-refraction plant tissue. The method comprises the following steps: (1) taking a to-be-observed part of a plant tissue, and preparing a plant tissue slide according to a microscopic slide preparation method under four microscopic identification methods (general rule 2001) of Chinese Pharmacopoeia in edition 2020; (2) taking the slide in the step (1), placing the slide under a polarized light microscope, and adjusting a basic imaging mechanism to be in single-refraction and double-refraction tissue structure common imaging mode; and (3) shooting an image by adopting a large-image splicing technology and a real-time depth-of-field extension imaging technology of a digital imaging system. According to the method, the defects that partial tissue structure characteristic information of a single-refraction microscopic image is lost under the traditional orthogonal polarized light condition, the microscopic image cannot quickly and accurately position a double-refraction microscopic characteristic marker under the normal light condition, and the definition and the integrity of the image are difficult to consider at the same time during microscopic shooting imaging are overcome.

A method of hair analysis based on microscope pictures of the hair in polarized light includes a hair conditioning phase performed in a container made of polyethylene or a polymer being in a position lower than polyethylene in the triboelectric series; this is followed by a hair testing phase in which polarized light microscope images of the hair, contacted with a turpentine oil, are taken and further processed using unmixing to obtain information on the amount of analytes present in the hair. Advantageously the present method shows high accuracy even in presence of accidental movements applied to the microscope/sample to be analyzed, such as possibly occurring during daily laboratory practice. Also advantageously, the high accuracy is maintained when different operators are involved in the procedure, being thus substantially unaffected by different ways of manipulating/preparing/photographing the hair sample.