32 results about "Polarized light microscopy" patented technology

Various embodiments of the present invention are directed to real-time capture, analysis, and output of polarizing microscopy images that quantify detected LB signals at discrete locations within the image. In one embodiment of the present invention, circularly polarized light is passed through a sample and optically imaged by traditional polarizing-light-microscope components. The resulting image is then split four ways and analyzed by a four-way polarizer / analyzer, and the four resulting analyzed subimages are computationally processed to produce three false-color, real-time images that represent per-pixel linear birefringence, extinction angle, and transmission at each position within a quarter-sized representation of the original image produced by conventional light-microscope imaging components. The false-color images can be produced at a rate of 30 frames per second or at greater rates by employing highly efficient image capture and computational processing of captured images through efficient programming techniques.

Apparatus for generating polychromatic polarized light with the polarization ellipse orientation determined by the wavelength. The proposed polychromatic polarization state generator can be used in various configurations of polarized light microscope (called “polychromatic polscope”) for imaging birefringent samples. The polychromatic polscope produces a spectral-modulated visual scene, in which birefringent structures are evident because their appearance is different from the background. New polarized light microscope can subtract the background and produce video-enhanced color image of birefringent structures. The obtained picture can be also mathematically processed in order to obtain a map of quantitative distribution of specimen retardation and orientation of the principal axes.

Disclosed is a method for measuring a fiber content of polyethyleneterephthalate (PET) and polytrimethyleneterephthalate (PTT) fibers, particularly wherein the method includes: a qualitative analysis step of identifying a presence of polyethyleneterephthalate (PET) and polytrimethyleneterephthalate (PTT) fibers in a mixed-spun fiber product; and a quantitative analysis step of measuring the fiber content by a microscopic count through difference in color between PET and PTT fibers by birefringence in a cross polarization state of a polarized light microscope (PLM). Through the present invention, it is possible to simply and accurately calculate the fiber content of PET and PTT fibers in a mixed-spun fiber material in which various kinds of fibers are mixed.

The invention relates to an intuitive detection method based on polarized light microscope observation of montmorillonite adsorbed lipids. First, montmorillonite is mixed with high-fat food or feed, and then the sample is made into a smear, and the neutral lipid dye oil Red 0 was used to stain the smear, and the prepared smear was detected under a polarized light microscope. Observe the adsorption properties of montmorillonite to lipids in high-fat food. The invention is a physical detection method, which can directly observe the adsorption of montmorillonite to lipid, and is simple in operation, intuitive and convenient.

The invention provides a staining method for observing collagen fibers with a polarized light microscope. The method is to stain with saturated picric acid-Sirius red staining solution at 70°C-90°C, then soak the tissue sections with a weak acid solution with a concentration of 1-10% w / v, and finally soak the tissue sections as usual. Washing, drying, and fixing; the heated picric acid has a stronger penetration effect on the tissue, making the background of the lining more vivid, and the heated Sirius red dye solution is also more firmly adsorbed on the collagen fibers, and the coloring is clear; the weak acid solution can be washed off The crystallization of picric acid precipitated in the tissue section is not easy to wash off the background of the yellow lining, so as to ensure that the collagen fibers are well colored, the background of the yellow lining is obvious, and the collagen fibers are clear; the dyeing method of the present invention has good repeatability, and the collagen fibers are colored Obviously, the fiber structure is clear. This method is conducive to the positioning and quantitative analysis of different types of collagen fibers, and is convenient for judging the degree of tissue fibrosis.

This invention relates to analogue blood bed display iron magnetic substance heating method, which comprises the following steps: quickly cooling it down and cracking it into parts; re-piecing the cracked blood cover slices on the glass slides with gaps between; dripping melted poly glycol between blood cover slice and glass slice; deleting the poly glycol in gaps after the poly glycol solidified; filling the dried iron magnetic power to the above gaps to get the analogue blood bed; putting the above analogue blood bed in the alternating magnetic field and then putting the analogue blood bed under bias microscopes for observation.

The invention discloses a crystal form X of rebaudioside E, a preparation method and application thereof, and belongs to the field of chemical pharmacy. The technology provided by the present invention can. The present invention utilizes low-carbon alcohol water as a solvent, utilizes rubus glycoside to solubilize, and crystallizes to obtain rebaudioside E crystals whose crystal form is X, and overcomes the low solubility of rebaudioside E in alcohol-water solvents, so that Difficulty in obtaining crystals in large quantities with high efficiency. Using technical means such as XRD, DSC, TGA and polarized light microscopy to characterize the rebaudioside E crystal with crystal form X, it was found that the rebaudioside E crystal with crystal form X had high crystallinity, stable Good sex and other advantages. The preparation method involved in the invention is simple, easy to operate, good in reproducibility, and can stably obtain target crystals in batches.