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Method for identifying mineral drugs based on polarized light microscopy technology

A microscopic technology and mineral medicine technology, which is applied in the direction of material analysis, polarization influence characteristics, and measuring devices through optical means, can solve the problem of difficult to clearly observe surface texture and fine structure, lack of microscopic identification research of mineral medicine, Problems such as inability to accurately locate birefringent mineral structures

Pending Publication Date: 2020-10-27
SHANGHAI UNIV OF T C M
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Problems solved by technology

However, there is a lack of research on microscopic identification of mineral drugs in the previous editions of "Chinese Pharmacopoeia" and literature records, and most of the existing microscopic identification techniques are observed under normal light and orthogonally polarized light, but observation under normal light Panlong jade, Panlong black jade, and mineral grinding slices all exhibit monochromatic phenomena, and the birefringent mineral structure cannot be accurately located; although observation under orthogonal polarized light can clearly and intuitively locate the birefringent mineral structure, it can also show various parts of different parts. Anisotropy, but it is difficult to clearly observe its surface texture and fine structure

Method used

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  • Method for identifying mineral drugs based on polarized light microscopy technology
  • Method for identifying mineral drugs based on polarized light microscopy technology

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Embodiment 1

[0019] Two kinds of jade, Panlong black jade and Panlong jade, and four mineral medicines of mica, white quartz, calcite and chlorite were prepared by grinding method. The specific steps are: select the test material with a thickness of 1-2mm, place On the grinding stone, add appropriate amount of water, hand-press the material on the grinding stone to grind back and forth, when both sides are smooth and the thickness is about hundreds of microns, transfer the material to the fine grinding stone, add water, press the material with a cork to grind back and forth until transparent, rinse with water , and then treated with ethanol and loaded with glycerol ethanol test solution. Place the prepared polished slides under a polarized light microscope with a compensator, and use the normal light microscopic identification technology, polarized light and semi-polarized light microscopic identification technology to test the normal light, orthogonally polarized light, and specific polari...

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Abstract

The invention relates to the field of polarized light detectionand particularly relates to a method for identifying mineral drugs based on a polarized light microscopy technology. The method comprisesthe following steps of (1) putting mineral medicine with the thickness of 1-2mm on a coarse grinding stone, adding a proper amount of water, and grinding the mineral medicine back and forth on the coarse grinding stone until the two sides are ground to be smooth and the thickness is 0.2-0.9 mm; (2) transferring the mineral medicine ground in the step (1) to a fine grinding stone, adding the water, grinding the mineral medicine back and forth till the mineral medicine is transparent, washing the ground mineral medicine with water, treating the ground mineral medicine with ethanol, and fillingthe ground mineral medicine with a glyceryl ethanol test solution; and (3) putting the abrasive disc prepared in the step (2) under a polarized light microscope with a compensator, setting the angle of semi-polarized light, and shooting under a 10-time objective lens by adopting a large-image splicing technology in combination with a real-time depth-of-field extended imaging technology to obtain color image data. According to the method, the birefringent mineral structure can be accurately positioned, and the multi-color property of anisotropy of structures of different parts of the mineral under polarized light is fully displayed.

Description

technical field [0001] The invention relates to the field of polarized light detection, in particular to a method for identifying mineral medicine based on polarized light microscopy. Background technique [0002] The properties and components of jade and mineral medicines are different due to the influence of geological and physical and chemical conditions during the formation process, and their identification work is difficult. Relying on its unique advantages of intuition, simplicity, speed, ease, economy, and environmental protection, microscopic identification has been widely used in the identification of traditional Chinese medicine. However, there is a lack of research on microscopic identification of mineral drugs in the previous editions of "Chinese Pharmacopoeia" and literature records, and most of the existing microscopic identification techniques are observed under normal light and orthogonally polarized light, but observation under normal light Panlong jade, Pa...

Claims

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Application Information

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IPC IPC(8): G01N21/21G01N21/23
CPCG01N21/21G01N21/23
Inventor 崔亚君马颖娴韩文凯张彤徐宏喜
Owner SHANGHAI UNIV OF T C M
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